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The Effect of Selenium on Endometrial Repair in Goats with Endometritis at High Cortisol Levels

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Abstract

Endometritis is a common postpartum disease of female animals that causes significant losses to the goat industry. High levels of cortisol induced by various stresses after delivery severely inhibit innate immunity and tissue repair. The repair ability of the endometrium is closely related to the reproductive performance of goats. Selenium (Se) is an essential trace element in animals that has powerful antioxidant and immunity-enhancing functions. In this study, we established a goat model of endometritis at high cortisol (Hydrocortisone) levels to investigate the effect of Se (supplement additive) on endometrial repair. The results showed that the clinical symptoms, %PMN in uterine secretions, morphological endometrial damage, and the gene expression of BAX were reduced in the goats with Se supplementation compared with those in the model group. Se increased the gene expression of BCL2, VEGFA, TGFB1, and PCNA and activated the PI3K/AKT and Wnt/β-catenin signaling pathways in goats with Se supplementation. In conclusion, Se reduced the inflammatory response, increased the proliferation, and decreased the apoptosis of endometrial cells to promote endometrial tissue repair in goats with endometritis at high cortisol levels. It probably achieved this effect of promoting repair by activating the Wnt/β-catenin and PI3K/AKT pathways and affecting the gene expression of VEGFA, TGFB1, PCNA, BCL2, and BAX.

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Data Availability

The datasets analyzed during the current study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by the National Natural Science Foundation of China (32072937, 32102735), the Natural Science Foundation of Jiangsu Province (BK20210808), Postgraduate Research & Practice Innovation Program of Jiangsu Province (Yangzhou University) (SJCX21_1637), the Earmarked Fund for Jiangsu Agricultural Industry Technology System (JATS[2022]499), the 333 High-level Talent Training Project of Jiangsu Province (CN), the 111 Project (D18007), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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  1. Hanqing Li and Changning Yuan contributed equally to this work.

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  1. College of Veterinary Medicine, Yangzhou University, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China

    Hanqing Li, Changning Yuan, Heng Wang, Luying Cui, Kangjun Liu, Long Guo, Jianji Li & Junsheng Dong

  2. Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou, 225009, China

    Hanqing Li, Changning Yuan, Heng Wang, Luying Cui, Kangjun Liu, Long Guo, Jianji Li & Junsheng Dong

  3. International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou, 225009, China

    Hanqing Li, Changning Yuan, Heng Wang, Luying Cui, Kangjun Liu, Long Guo, Jianji Li & Junsheng Dong

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  1. Hanqing Li
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Contributions

HL: conceptualization, writing – original draft. CY: methodology. HW: supervision. LC, KL, and LG: formal analysis. JD: writing – review and editing, funding acquisition, supervision. JL: funding acquisition, supervision.

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Correspondence to Jianji Li or Junsheng Dong.

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All experimental procedures were approved by the Animal Care and Use Committee of Yangzhou University (approval ID: 20190303). The animal experimental procedures conducted were in accordance with the Guide for Care and Use of Agricultural Animals of Yangzhou University.

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Li, H., Yuan, C., Wang, H. et al. The Effect of Selenium on Endometrial Repair in Goats with Endometritis at High Cortisol Levels. Biol Trace Elem Res 202, 2564–2576 (2024). https://doi.org/10.1007/s12011-023-03866-y

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