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Zinc Ameliorates Tripterygium Glycosides–Induced Reproductive Impairment in Male Rats by Regulating Zinc Homeostasis and Expression of Oxidative Stress–Related Genes

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Abstract

Tripterygium glycosides (TG) can seriously damage male reproductive function, and the reproductive system is difficult to restore after stopping the administration of TG in male rats. Zinc (Zn) is one of the most important trace elements in the human body and plays an important role in maintaining male fertility. The aim of this study was to investigate whether zinc supplementation could improve the testicular reproductive damage induced by TG toxicity in rats and to investigate its mechanism of action. The results showed that zinc sulfate (ZnSO4) could improve testicular tissue structure and semen parameters, promote testosterone synthesis, increase zinc-containing enzyme activity, increase zinc concentration in serum and testicular tissues, and maintain zinc homeostasis in male rats induced by TG toxicity. Zinc supplementation activated relevant signalling molecules in the KEAP1-NRF2/ARE pathway and alleviated TG-induced oxidative stress. Therefore, this study concluded that zinc supplementation could improve reproductive damage by regulating zinc homeostasis and the expression of genes related to oxidative stress.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by S&T Program of Hebei (Grant No. 203777107D); Hebei Natural Science Foundation (Grant No.H2022314001);

Science and Technology Department of Hebei Province (project number: 22567604D).

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Authors and Affiliations

  1. Hebei Key Laboratory of Reproductive Medicine, Hebei Reproductive Health Hospital, No. 80 Heping Street, Xinhua District, Shijiazhuang, 050071, China

    Jing Ma, Yingxian Zhen & Shusong Wang

  2. Hebei General Hospital, Shijiazhuang, 050051, China

    He Tan

  3. Graduate School of Hebei Medical University, Shijiazhuang, 050017, China

    He Tan, Bo Sun & Shusong Wang

  4. Graduate School of Chengde Medical University, Chengde, 067000, China

    Jiajie Bi & Shusong Wang

  5. Department of Laboratory Animals, The Key Lab of Hebei Provincial Laboratory Animals, Hebei Medical University, Shijiazhuang, 050017, China

    Weiguang Lian

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Contributions

Weiguang Lian raised experimental animals. He Tan and Bo Sun performed the experiments. Jiajie Bi analyzed the data. Yingxian Zhen collated the data. Jing Ma and Jiajie Bi wrote the manuscript. Jing Ma and Shusong Wang conceived the idea, designed the study, and revised the manuscript. Shusong Wang, Weiguang Lian, and Yingxian Zhen collected the funds. All authors read and approved the final version of the manuscript.

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Ma, J., Tan, H., Bi, J. et al. Zinc Ameliorates Tripterygium Glycosides–Induced Reproductive Impairment in Male Rats by Regulating Zinc Homeostasis and Expression of Oxidative Stress–Related Genes. Biol Trace Elem Res 202, 2111–2123 (2024). https://doi.org/10.1007/s12011-023-03815-9

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