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Improved Biocompatibility and Angiogenesis of the Bone Titanium Scaffold through ERK1/2 Signaling Mediated by an Attached Strontium Element

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Abstract

The promotion of early osseointegration is crucial for the success of biomedical titanium implants. Physical and chemical modifications to the material surface can significantly compensate for the lack of biocompatibility and early osseointegration of the implant. In this study, we implanted strontium onto titanium plates and analyzed the effect of strontium-doped materials on angiogenesis and biocompatibility in the human bone structure. Our findings demonstrated that strontium-loaded titanium sheet materials effectively promote human umbilical vein endothelial cell (HUVEC) biocompatibility and vascular differentiation ability, as evidenced by proliferation-apoptosis assays, RT-qPCR for vascular neogenesis markers, ELISA for vascular endothelial growth factor (VEGF) levels, and nitric oxide (NO) analysis. Mechanism studies based on RNAseq and Western blotting analysis revealed that strontium can promote titanium material biocompatibility with HUVEC cells and vascular neovascularization ability by activating the extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway. Meanwhile, blocking the ERK1/2 signaling pathway could reverse the promotional effect of vascular formation. Overall, we have successfully fabricated a multifunctional biocompatible bone implant with better histocompatibility and angiogenesis compared to uncoated implants.

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Data Availability

The data that support the finding of this study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by Natural Science Foundation of Hebei Province Youth Science Foundation Project (Grant numbers H2020109157).

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Handan Central Hospital, Handan, HeBei, China

    Bingkun Cheng, Xueqiang Zhang, Qingqing Cui, Chao Ma & Jianjun Jiao

  2. Department of Stomatology, Handan Central Hospital, Handan, HeBei, China

    Qing Yong Chen & Jiahuan He

Authors
  1. Bingkun Cheng
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  2. Qing Yong Chen
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  3. Xueqiang Zhang
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  4. Jiahuan He
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  5. Qingqing Cui
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  6. Chao Ma
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  7. Jianjun Jiao
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Jianjun Jiao, Bingkun Cheng, QingYong Chen, Jiahuan He, and Xueqiang Zhang. The first draft of the manuscript was written by Bingkun Cheng, Jiahuan He, Qingqing Cui, and Chao Ma. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Jianjun Jiao.

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Cheng, B., Chen, Q.Y., Zhang, X. et al. Improved Biocompatibility and Angiogenesis of the Bone Titanium Scaffold through ERK1/2 Signaling Mediated by an Attached Strontium Element. Biol Trace Elem Res 202, 1559–1567 (2024). https://doi.org/10.1007/s12011-023-03772-3

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