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Selenium Deficiency Causes Iron Death and Inflammatory Injury Through Oxidative Stress in the Mice Gastric Mucosa

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Abstract

Selenium (Se) is a trace element essential for the maintenance of normal physiological functions in living organisms. Oxidative stress is a state in which there is an imbalance between oxidative and antioxidant effects in the body. A deficiency of Se can make the body more inclined to oxidation, which can induce related diseases. The aim of this experimental study was to investigate the mechanisms by which Se deficiency affects the digestive system through oxidation. The results showed that Se deficiency treatment led to a decrease in the levels of GPX4 and antioxidant enzymes and an increase in the levels of ROS, MDA, and lipid peroxide (LPO) in the gastric mucosa. Oxidative stress was activated. Triple stimulation of ROS, Fe2+, and LPO induced iron death. The TLR4/NF-κB signaling pathway was activated, inducing an inflammatory response. The expression of the BCL family and caspase family genes was increased, leading to apoptotic cell death. Meanwhile, the RIP3/MLKL signaling pathway was activated, leading to cell necrosis. Taken together, Se deficiency can induce iron death through oxidative stress. Meanwhile, the production of large amounts of ROS activated the TLR4/NF-κB signaling pathway, leading to apoptosis and necrosis of the gastric mucosa.

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Funding

This study was supported by the Natural Science Foundation of Heilongjiang (YQ2021C022) and the postdoctoral scientific research developmental fund of Heilongjiang (LBH-Q19080).

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Authors and Affiliations

  1. Laboratory of Animal Physiology, College of Veterinary Medicine, Northeastern Agricultural University, Harbin, Heilongjiang Province, People’s Republic of China

    Shuang Xu, Kan Li, Xueying Li, Yanhe Zhang & Xue-Jiao Gao

  2. Animal Disease Prevention and Control Center of Heilongjiang Province, Harbin, 150000, People’s Republic of China

    Zibo Kang & Xue-Jiao Gao

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  1. Shuang Xu
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  2. Zibo Kang
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  3. Kan Li
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  4. Xueying Li
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Contributions

All authors contributed to the research and design of this article. Xue-jiao Gao and Shuang Xu conceived and designed the experiments. Shuang Xu, Zibo Kang, and Kan Li carried out the experiments. Shuang Xu and Xueying Li processed the data. Shuang Xu and Xue-jiao Gao wrote the paper. All authors have reviewed this manuscript.

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Correspondence to Xue-Jiao Gao.

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All animal experiments involved in this paper were approved by the Animal Research Ethics Committee of Northeastern Agricultural University (NEAUEC20220340).

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Xu, S., Kang, Z., Li, K. et al. Selenium Deficiency Causes Iron Death and Inflammatory Injury Through Oxidative Stress in the Mice Gastric Mucosa. Biol Trace Elem Res 202, 1150–1163 (2024). https://doi.org/10.1007/s12011-023-03754-5

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