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Quantitative Proteomics Reveals Manganese Alleviates Heat Stress of Broiler Myocardial Cells via Regulating Nucleic Acid Metabolism

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Abstract

Heat stress threatens severely cardiac function by caused myocardial injury in poultry. Our previous study has showed that manganese (Mn) has a beneficial effect on heat-stress resistance of broiler. Therefore, we tried to confirm the alleviation mechanism through proteomic analysis after heat stress exposure to primary broiler myocardial cells pretreated with Mn. The experiment was divided into four groups: CON group (37 °C, cells without any treatment), HS group (43 °C, cells treatment with heat stress for 4 h), HS+MnCl2 group (cells treated with 20 μM MnCl2 before heat stress), and HS+Mn-AA group (cells treated with 20 μM Mn compound amino acid complex before heat stress). Proteome analysis using DIA identified 300 differentially expressed proteins (DEPs) between CON group and HS group; 93 and 121 DEPs were identified in inorganic manganese treatment group and organic manganese treatment group, respectively; in addition, there were 53 DEPs identified between inorganic and organic manganese group. Gene Ontology (GO) analysis showed that DEPs were mainly involved in binding, catalytic activity, response to stimulus, and metabolic process. DEPs of manganese pretreatment involved in a variety of biological regulatory pathways, and significantly influenced protein processing and repair in endoplasmic reticulum, apoptosis, and DNA replication and repair. These all seem to imply that manganese may help to resist cell damage induced by heat stress by regulating key node proteins. These findings contribute to a better understanding of the effects of manganese on overall protein changes during heat-stress and the possible mechanisms, as well as how to better use manganese to protect heart function in high temperature.

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Funding

This research was funded by the National Natural Science Foundation of China (No. 31960668) and Discipline Team Project of Gansu Agricultural University (NO: GAU-XKTD-2022-24).

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  1. Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, People’s Republic of China

    Shizhen Qin, Rui Wang, Jinlu Li, Defu Tang & Zhaoguo Shi

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  1. Shizhen Qin
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  2. Rui Wang
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Contributions

Shizhen Qin: conceptualization, methodology, writing—original draft preparation; Rui Wang: conceptualization, methodology, software; Jinlu Li: contributed materials, data curation, methodology; Defu Tang: methodology, software; Zhaoguo Shi: project administration, methodology.

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Correspondence to Zhaoguo Shi.

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Qin, S., Wang, R., Li, J. et al. Quantitative Proteomics Reveals Manganese Alleviates Heat Stress of Broiler Myocardial Cells via Regulating Nucleic Acid Metabolism. Biol Trace Elem Res 202, 1187–1202 (2024). https://doi.org/10.1007/s12011-023-03731-y

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