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Selenium-Enriched Cardamine violifolia Alleviates LPS-Induced Hepatic Damage and Inflammation by Suppressing TLR4/NODs–Necroptosis Signal Axes in Piglets

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Abstract

Selenium-enriched Cardamine violifolia (SEC), a cruciferous plant, exerts excellent antioxidant and anti-inflammatory capacity, but its effect on hepatic function is unclear. This study investigated the effect and potential mechanism of SEC on hepatic injury induced by lipopolysaccharide (LPS). Twenty-four weaned piglets were randomly allotted to treatment with SEC (0.3 mg/kg Se) and/or LPS (100 μg/kg). After 28 days of the trial, pigs were injected with LPS to induce hepatic injury. These results indicated that SEC supplementation attenuated LPS-induced hepatic morphological injury and reduced aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities in plasma. SEC also inhibited the expression of pro-inflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) after the LPS challenge. In addition, SEC improved hepatic antioxidant capacity via enhancing glutathione peroxidase (GSH-Px) activity and decreasing malondialdehyde (MDA) concentration. Moreover, SEC downregulated the mRNA expression of hepatic myeloid differentiation factor 88 (MyD88) and nucleotide-binding oligomerization domain proteins 1 (NOD1) and its adaptor molecule receptor interacting protein kinase 2 (RIPK2). SEC also alleviated LPS-induced hepatic necroptosis by inhibiting RIPK1, RIPK3, and mixed-lineage kinase domain-like (MLKL) expression. These data suggest that SEC potentially mitigates LPS-induced hepatic injury via inhibiting Toll-like receptor 4 (TLR4)/NOD2 and necroptosis signaling pathways in weaned piglets.

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Data Availability

The data are available from the corresponding author.

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Funding

This research was financially supported by the Project of the National Natural Science Foundation of China (no. U22A20517, no. 32272906, and no. 32102566) and the Scientific Research Project of Wuhan Polytechnic University (no. 2022J06).

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Authors and Affiliations

  1. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China

    Dan Wang, Wenshuai Xie, Wensheng He, Huiling Zhu & Yulan Liu

  2. Enshi Se-Run Material Engineering Technology Co., Ltd., Enshi, 445000, China

    Yue Zhang, Qingyu Gao & Xin Cong

  3. National R&D Center for Se-rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China

    Shuiyuan Cheng

Authors
  1. Dan Wang
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  2. Wenshuai Xie
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  4. Huiling Zhu
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  5. Yue Zhang
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  7. Xin Cong
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  9. Yulan Liu
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Contributions

DW and YL designed the study and acquired funding. DW, WX, and YL wrote the manuscript. WX, WH, HZ, and XC conducted the animal trials. XC, YZ, QG, and SC analyzed the parameters and data. DW and YL read and approved the final version.

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Correspondence to Yulan Liu.

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Dan Wang and Wenshuai Xie share co-first authorship.

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Wang, D., Xie, W., He, W. et al. Selenium-Enriched Cardamine violifolia Alleviates LPS-Induced Hepatic Damage and Inflammation by Suppressing TLR4/NODs–Necroptosis Signal Axes in Piglets. Biol Trace Elem Res 202, 527–537 (2024). https://doi.org/10.1007/s12011-023-03713-0

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