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Nano-selenium Antagonizes Heat Stress-Induced Apoptosis of Rainbow Trout (Oncorhynchus mykiss) Hepatocytes by Activating the PI3K/AKT Pathway

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Abstract

The cold-water fish rainbow trout (Oncorhynchus mykiss) shows poor resistance to heat, which is the main factor restricting their survival and yield. With the advancement of nanotechnology, nano-selenium (nano-Se) has emerged as a key nano-trace element, showing unique advantages, including high biological activity and low toxicity, for studying the response of animals to adverse environmental conditions. However, little is still known regarding the potential protective mechanisms of nano-Se against heat stress-induced cellular damage. Herein, we aimed to investigate the mechanism underlying the antagonistic effects of nano-Se on heat stress. Four groups were assessed: CG18 (0 μg/mL nano-Se, 18 °C), Se18 (5.0 μg/mL nano-Se, 18 °C), CG24 (0 μg/mL nano-Se, incubated at 18 °C for 24 h and then transferred to 24 °C culture), and Se24 (5.0 μg/mL nano-Se, incubated at 18 °C for 24 h and then transferred to 24 °C culture). We found that after heat treatment (CG24 group), T-AOC, GPx, and CAT activities in rainbow trout hepatocytes showed a decrease of 36%, 33%, and 19%, respectively, while ROS and MDA levels showed an increase of 67% and 93%, respectively (P < 0.05). Meanwhile, the mRNA levels of the apoptosis-related genes caspase3, caspase9, Cyt-c, Bax, and Bax/Bcl-2 in the CG24 group were 41%, 47%, 285%, 65%, and 151% higher than those in the CG18 group, respectively, while those of PI3K and AKT were 31% and 17% lower, respectively (P < 0.05). Besides, flow cytometry analysis showed an increase in the level of apoptotic cells after heat exposure. More importantly, we observed that nano-Se cotreatment (Se24 group) remarkably attenuated heat stress-induced effects (P < 0.05). We conclude that heat stress induces oxidative stress and apoptosis in rainbow trout hepatocytes. Nano-Se ameliorates heat stress-induced apoptosis by activating the PI3K/AKT pathway. Our results provide a new perspective to improve our understanding of the ability of nano-Se to confer heat stress resistance.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 31960727).

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  1. College of Animal Science & Technology, Gansu Agricultural University, No. 1 Yingmen Village, Anning District, Lanzhou, 730070, Gansu Province, China

    Lanlan Li, Zhe Liu, Guiyan Zhao, Jinqiang Quan, Jun Sun & Junhao Lu

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  1. Lanlan Li
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  2. Zhe Liu
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  3. Guiyan Zhao
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  5. Jun Sun
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Contributions

Lanlan Li is responsible for the conceptualization, methodology, writing—original draft, and writing—review and editing. Zhe Liu did writing—review and editing—and supervision. Jinqiang Quan is assigned to data curation. Jun Sun is assigned to methodology and investigation. Guiyan Zhao is involved in data curation, software, and conceptualization. Junhao Lu is the checking. All authors approved the final manuscript.

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Correspondence to Zhe Liu.

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All the experiments were performed in accordance with the Chinese Legislation on the Use and Care of Laboratory Animals, and the protocol was approved by the Ethics Committee at Gansu Agricultural University (No. GSAU-2019–52).

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Li, L., Liu, Z., Zhao, G. et al. Nano-selenium Antagonizes Heat Stress-Induced Apoptosis of Rainbow Trout (Oncorhynchus mykiss) Hepatocytes by Activating the PI3K/AKT Pathway. Biol Trace Elem Res 201, 5805–5815 (2023). https://doi.org/10.1007/s12011-023-03637-9

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