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Therapeutic Potential of Selenium Nanoparticles on Letrozole-Induced Polycystic Ovarian Syndrome in Female Wistar Rats

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Abstract

Polycystic ovarian syndrome (PCOS) is considered the most frequent gynecological endocrine disorder that causes anovulatory infertility. The current study aimed to investigate the potential significance of selenium nanoparticles (SeNPs), an IL-1 inhibitor, in the treatment of letrozole-induced PCOS in rats that satisfied the metabolic and endocrine parameters found in PCOS patients. Letrozole (2 ppm, per orally, p.o.) was given orally to female Wistar rats for 21 days to develop PCOS. After PCOS induction, rats were given SeNPs (25 ppm/day, p.o.), SeNPs (50 ppm/day, p.o.), or metformin (2 ppm/day, p.o.) for 14 days. PCOS was associated with an increase in body weight, ovarian weight, ovarian size, and cysts, as well as an increase in blood testosterone, luteinizing hormone (LH), and insulin, glycaemia, and lipid profile levels. The SeNP administration decreased all of these variables. Furthermore, SeNPs significantly reduced letrozole-induced oxidative stress in the ovaries, muscles, and liver by decreasing elevated levels of malondialdehyde and total nitrite while raising suppressed levels of superoxide dismutase and catalase. SeNPs increased the amounts of the protective proteins Kelch-like ECH-associated protein 1 (Keap-1), nuclear factor erythroid 2–related factor 2 (Nrf2), and OH-1. It was depicted from the study that SeNPs reduce the upregulation of inflammatory cytokines that are interleukin 6 (IL-6), tumour necrosis factor α (TNF-α), and the interleukin 1 (IL-1). Our findings show that SeNPs, through their antioxidant and anti-inflammatory characteristics, alleviate letrozole-induced PCOS.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

Present article is extracted from Ph.D. dissertation research work of first author Miss. Maisra Azhar Butt. Strong acknowledgment is for International Islamic University Islamabad, Pakistan, and Shifa Tameer-e-Millat University Islamabad, Pakistan, who provided facilities for conduct of this research.

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Authors and Affiliations

  1. Department of Bioinformatics and Biotechnology, International Islamic University, Islamabad, Pakistan

    Maisra Azhar Butt, Anum Munir & Sobia Tabassum

  2. Department of Cardiology, PAF Hospital, Islamabad, E-9, Pakistan

    Hafiz Muhammad Shafique

  3. ARL Hospital Morgah, Rawalpindi, Pakistan

    Mahjabeen Mustafa

  4. Rawal Institute of Health Sciences, Shaheed Zulfiqar Ali Bhutto Medical University (SZABMU), Islamabad, Pakistan

    Nurain Baig Moghul

  5. Department of Biological Sciences, Quaid-I-Azam University, Islamabad, Pakistan

    Urwah Shamas

  6. Shifa College of Medical Technology, Shifa Tameer-E-Millat University, Islamabad, Pakistan

    Mubin Mustafa Kiyani

  7. Shifa International Hospitals Ltd، Gate No, Islamabad Capital Territory, 1، 4 Pitras Bukhari Rd, H 8/4 H-8, Islamabad, Pakistan

    Mubin Mustafa Kiyani

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S. no

Contributions

Names of authors

 1.

Study conception or design

Maisra Azhar Butt, Mubin Mustafa Kiyani

 2.

Data processing, collection, perform experiment

Hafiz Muhammad Shafique, Urwah Shamas

 3.

Analysis and interpretation of results

Anum Munir, Mahjabeen Mustafa

 4.

Critical revision or editing of the article

Nurain Baig Moghul, Sobia Tabassum

 5.

Final approval of the version to be published

Maisra Azhar Butt, Hafiz Muhammad Shafique, Urwah Shamas, Mahjabeen Mustafa, Nurain Baig Moghul, Anum Munir, Sobia Tabassum, and Mubin Mustafa Kiyani

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Correspondence to Mubin Mustafa Kiyani.

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Highlights

Selenium nanoparticles (SeNPs) were prepared, characterized, and administered to letrozole-induced PCOS rats.

Different biochemical tests were performed to evaluate the effect of SeNPs.

SeNPs increased the concentration of antioxidant enzymes and reduced the concentration of cholesterol and inflammatory proteins.

SeNPs decreased the antioxidant stress produced by the inflammatory markers IL-1 and IL-6.

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Butt, M.A., Shafique, H.M., Mustafa, M. et al. Therapeutic Potential of Selenium Nanoparticles on Letrozole-Induced Polycystic Ovarian Syndrome in Female Wistar Rats. Biol Trace Elem Res 201, 5213–5229 (2023). https://doi.org/10.1007/s12011-023-03579-2

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  • DOI: https://doi.org/10.1007/s12011-023-03579-2

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