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Microstructural Analysis of Cancellous Bone in Fluorosis Rats

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Abstract

Skeletal fluorosis likely alters bone structural properties on the cortical and cancellous tissue levels in view that fluorine ion replaces bone mineral composition. Our previous study showed high bone turnover occurred in cortical bone of skeletal fluorosis. Therefore, this study further analyzed the microstructure of cancellous bone in fluorosis rats. Rats were randomly assigned into three groups: the control, low-dose fluoride group (10 mgF-/kg·day), and high-dose fluoride group (20 mgF-/kg·day). Rats were orally administered with fluoride for 1, 2, and 3 months of periods. The trabecular bone parameters of tibia were detected with micro CT and analyzed with software. The activities of glutathione peroxidase (GPX), superoxide dismutase (SOD), and the content of malondialdehyde (MDA) in serum were measured. Results showed that severity of dental fluorosis rose with the increase of dose and prolongation of fluoride exposure. Meantime, the poorer connectivity and less trabecular bone network were observed in cancellous bone of rats treated with fluoride. Data analysis indicated that fluoride treatment significantly decreased bone volume and connectivity degree, but amplified trabecular space in 1 and 2 months of periods. Intriguingly, trabecular thickness significantly decreased in 1-month high-dose fluoride group, but returned to the control in 3 months of period. Fluoride treatment mainly inhibited the GPX activity and increased the MDA level to activate oxidative stress. This study confirmed that excessive fluoride impaired cancellous bone and caused redox imbalance.

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Data Availability

Some or all data and models during the study are available from the corresponding author on reasonable request.

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Funding

This research is supported by the National Natural Science Foundation of China [Grant No. 81673111].

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Authors and Affiliations

  1. The affiliated hospital of Changchun university of Chinese medicine, Changchun, 130021, People’s Republic of China

    Haiyan Li

  2. School of Pharmaceutical Sciences, Jilin University, Changchun, 130021, People’s Republic of China

    Xiaoxue Chen, Zhenhua Zhang, Jingmin Zhang & Hui Xu

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  1. Haiyan Li
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  2. Xiaoxue Chen
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  3. Zhenhua Zhang
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  4. Jingmin Zhang
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  5. Hui Xu
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Contributions

H.L.: writing—original draft preparation, methodology. X.C.: investigation and validation; Z.Z.: data validation. J.Z., the conception of the study. H.X.: writing—review and editing, data curation, resources project administration, and funding acquisition. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Hui Xu.

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Li, H., Chen, X., Zhang, Z. et al. Microstructural Analysis of Cancellous Bone in Fluorosis Rats. Biol Trace Elem Res 201, 4827–4833 (2023). https://doi.org/10.1007/s12011-023-03564-9

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  • DOI: https://doi.org/10.1007/s12011-023-03564-9

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