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Effects of Iron-Peptides Chelate Nanoliposomes on Iron Supplementation in Rats

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Abstract

The objective of this study was to investigate the effects of iron nanoliposomes on iron supplementation and toxicity in SD rats induced by a low-iron diet. The size and infrared spectroscopy of a liposomal oral delivery system were investigated. The particle size of nanoliposomes embedded with chelates was increased. Infrared spectra proved that peptides-iron and blank nanoliposomes were bonded by interaction forces, including the fracture of hydrogen bonds, C = C bonds, hydrophobic interaction, and C-N bonds. We found that iron supplementation chelates had a certain protective effect on viscera after being embedded by nanoliposomes. After 10 days of treatment, the concentration of hemoglobin could be gradually increased. Nanoliposome encapsulated peptides-iron has a better effect than other groups. At the same time, SOD, MDA, and CAT reached normal levels after 20 days. Histological results showed that the sections of the nanoliposomes groups were clearer than those of the other groups. There was a little inflammation in the liver without obvious pathological changes, which also proved that the iron chelates embedded by nanoliposomes had no obvious side effects on iron supplementation in rats. Nanoliposome encapsulated peptides-iron has a small side effect and a significant curative effect of iron supplementation. It maybe has a good application prospect in the clinical medical field.

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Data Availability

All data reported in this study are available upon request by contact with the corresponding author.

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Funding

This research is supported by the Science and Technology Project of Zhejiang, Province, China [grant number LGN19C200018] and the Fundamental Research Funds for the Provincial Universities of Zhejiang [No 2020YW34].

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Authors and Affiliations

  1. Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou, 310018, China

    Mengqian Chen, Cen Chen, Yuhang Zhang, Han Jiang, YiZhou Fang & Guangrong Huang

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  1. Mengqian Chen
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  2. Cen Chen
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  3. Yuhang Zhang
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  4. Han Jiang
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Contributions

All authors contributed to the study conception and design. Methodology and validation were performed by Mengqian Chen and Guangrong Huang. The first draft of the manuscript was written by Mengqian Chen and Cen Chen, visualization and chart making was performed by Yuhang Zhang, Han Jiang, YiZhou Fang, writing-reviewing and editing were performed by Guangrong Huang. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Guangrong Huang.

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All animals were kept in a pathogen-free environment and fed ad lib. The procedures for care and use of animals were approved by the Ethics Committee of the China Jiliang University and all applicable institutional and governmental regulations concerning the ethical use of animals were followed.

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Chen, M., Chen, C., Zhang, Y. et al. Effects of Iron-Peptides Chelate Nanoliposomes on Iron Supplementation in Rats. Biol Trace Elem Res 201, 4508–4517 (2023). https://doi.org/10.1007/s12011-022-03539-2

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