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Biogenic Selenium Nanoparticles Synthesized by Lactobacillus casei ATCC 393 Alleviate Acute Hypobaric Hypoxia-Induced Intestinal Barrier Dysfunction in C57BL/6 Mice

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Abstract

Exposure to hypobaric hypoxia at high altitude will cause different tissue and organ damage over a long period of time. Studies have shown that hypobaric hypoxia can cause severe primary intestinal barrier dysfunction, and then cause multiple organ dysfunction. Our previous research showed that selenium nanoparticles (SeNPs) synthesized by Lactobacillus casei ATCC 393 (L. casei ATCC 393) can effectively alleviate intestinal barrier dysfunction caused by oxidative stress and inflammation in mice. This study was conducted to investigate the protective effect of biological SeNPs synthesized by L. casei ATCC 393 on intestinal barrier function in acute hypobaric hypoxic stress mice. The results showed that compared with the hypobaric hypoxic, the SeNPs synthesized by L. casei ATCC 393 by oral administration could effectively alleviate the shortening of intestinal villi, which decreased the level of diamine oxidase (DAO) and myeloperoxidase (MPO), and the expression level of tight junction protein in ileum was increased. In addition, SeNPs significantly increased the activities of superoxide dismutase (SOD), cyclooxygenase (COX-1) and glutathione peroxidase (GPx), and decreased the level of malondialdehyde (MDA), and inhibit the increase of hypoxia related factor. SeNPs effectively regulate the intestinal microecology disorder caused by hypobaric hypoxia stress, and maintain the intestinal microecology balance. In addition, oral administration of SeNPs had better protective effect on intestinal barrier function of mice under hypobaric hypoxia stress. These results suggested that SeNPs synthesized by L. casei ATCC 393 can effectively alleviate the damage of intestinal barrier function under acute hypobaric hypoxic stress, which may be closely related to the antioxidant activity of SeNPs.

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Funding

This study received the following funding sources and grant support: National Natural Science Foundation of China (No. 32072746), and the Key Research and Development Program of Shaanxi Province (No. 2021NY-004), and the Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University (No. CX2021029), and the Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University (No. CX2022062).

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Authors and Affiliations

  1. The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyixi Road Xi’an, Shaanxi, 710072, China

    Xina Dou, Baohua Zhang, Lei Qiao, Xiaofan Song, Shanyao Pi, Jiajing Chang, Xinyi Zhang, Xiaonan Zeng, Lixu Zhu & Chunlan Xu

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  1. Xina Dou
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Contributions

Chunlan Xu, Xina Dou, and Baohua Zhang contributed to the study conception and design. Xina Dou, Baohua Zhang, and Lei Qiao collected and analyzed the data. Jiajing Chang, Xiaofan Song, and Xinyi Zhang provided technical support. Xina Dou and Shanyao Pi drafted the manuscript. Chunlan Xu, Xiaofan Song, Xiaonan Zeng, and Lixu Zhu critically revised the manuscript to its final version. All authors contributed critically to the drafts and gave final approval for submission. Chunlan Xu is the guarantor.

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Correspondence to Chunlan Xu.

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Dou, X., Zhang, B., Qiao, L. et al. Biogenic Selenium Nanoparticles Synthesized by Lactobacillus casei ATCC 393 Alleviate Acute Hypobaric Hypoxia-Induced Intestinal Barrier Dysfunction in C57BL/6 Mice. Biol Trace Elem Res 201, 4484–4496 (2023). https://doi.org/10.1007/s12011-022-03513-y

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