Abstract
Chronic arsenic poisoning is a global health problem that affects millions of people, and studies have found that long-term ingestion of arsenic-containing compounds can lead to lung damage, but the exact mechanism is unknown. In this study, Sprague–Dawley (SD) rats were used as the research object, and the proteomic analysis method based on sequential window acquisition of all theoretical fragment ions (SWATH) was used to detect the changes in the expression levels of related proteins in the lung tissue of arsenic-exposed rats, and to explore the mechanism of arsenic compound-induced lung injury. The results showed that arsenic exposure resulted in the abnormal expression of collagen type III and proteins involved in metabolic, immune, and cellular processes, leading to the dysfunction of important pathways associated with these proteins, resulting in lung injury. It suggested that the underlying mechanism of arsenic-induced lung injury may be related to oxidative stress, immune injury, cell junction, and collagen type III. This result provides a new research idea for revealing the mechanism of lung injury caused by arsenic exposure.
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Data Availability
The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We are grateful to instrument analysis center of Shenzhen University and Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions.
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This work was supported by the National Natural Science Foundations of China (U1812403-6–2-4, 82173642), the Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions (2021SHIBS0003).
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LMS and PL conceived the experiment. YW designed the experiment. JZ, XLZ, HJZ, XSC, TH, JL, XXT, XLC, YXJ, XY, HBZ conducted experiments and analyzed data. LMS, PL, CXS, HJZ helped revise the manuscript. All authors have read and agree to the published version of the manuscript.
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All experimental procedures are in line with the guidelines for animal use and ethical care, and have been approved by the ethics committee of experimental animals of Guizhou Medical University. The quality certificate number is scxk (Guizhou) 2018–0001.
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Wang, Y., Zhang, J., Zhang, X. et al. Study on the Mechanism of Arsenic-Induced Lung Injury Based on SWATH Proteomics Technology. Biol Trace Elem Res 201, 3882–3902 (2023). https://doi.org/10.1007/s12011-022-03466-2
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DOI: https://doi.org/10.1007/s12011-022-03466-2