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Yttrium Oxide Nanoparticles Attenuate L-Arginine Induced Chronic Pancreatitis

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Abstract

In this work, we tested the efficacy of yttrium oxide nanoparticles (NY), a promising antioxidant and anti-inflammatory agent, in L-arginine (L-Arg) induced chronic pancreatitis (CP) model. The nanoparticles were characterized using multiple techniques including transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), powder X-ray diffraction (pXRD), and Energy dispersive X-ray analysis (EDX). The rats were divided into three groups: normal control, L-Arg control, L-Arg + NY (1 mg/kg). We probed the mechanistic effects of the NY by ELISA, multiplex analysis of TGF-β pathway and inflammatory cytokines and immunoblotting. NY treatment significantly reduced pancreatic oxidative-nitrosative stress. In addition, NY intervention also reduced inflammatory cytokines and chemokines resulting in the inhibition of fibrosis signaling. Further, NY treatment suppressed the TGF-β signaling and epithelial-mesenchymal transition (EMT). We conclude that NY shows potential antioxidant, anti-inflammatory, and anti-fibrotic effects against CP and associated fibrosis.

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Authors and Affiliations

  1. Department of Biological Sciences (Regulatory Toxicology), National Institute of Pharmaceutical Education and Research (NIPER), Balanagar, Hyderabad, Telangana, 500037, India

    Amit Khurana, Mohd Aslam Saifi & Chandraiah Godugu

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  1. Amit Khurana
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  2. Mohd Aslam Saifi
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  3. Chandraiah Godugu
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Contributions

AK performed experiments, collected and analyzed data, and wrote the manuscript. MAS helped in the experimental part and data collection. CG designed the study, supervised the work, and corrected the manuscript.

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Correspondence to Chandraiah Godugu.

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The present work complies to all the ethical practices. The experimental procedures were performed by following the ethical guidelines provided by CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals). The manuscript is the authors’ own original work, which has not been previously published elsewhere and the paper is not currently being considered for publication elsewhere. The manuscript reflects the authors’ own research and analysis in a truthful and complete manner.

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Khurana, A., Saifi, M.A. & Godugu, C. Yttrium Oxide Nanoparticles Attenuate L-Arginine Induced Chronic Pancreatitis. Biol Trace Elem Res 201, 3404–3417 (2023). https://doi.org/10.1007/s12011-022-03446-6

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