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Assessment of Tissue Oxidative Stress, Antioxidant Parameters, and Zinc and Copper Levels in Patients with Breast Cancer

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Abstract

Breast cancer is a multifactorial disease, and among the many factors which are involved in the onset, progression, and invasion of the disease, oxidative stress plays a significant role. The concentration and activity of enzymatic antioxidants are proportional to the concentration of trace elements, and the concentration of trace elements is often deficient in malignancies. Therefore, in the present study, we studied the tissue levels of oxidative stress, antioxidant status, zinc (Zn), and copper (Cu) in breast cancer patients. Tissue samples were collected from 40 patients with breast cancer and 40 tumor margin tissue as a control group. All subjects gave their informed consent. The tissue samples were measured for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), total antioxidants capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), malondialdehyde (MDA), Zn, and Cu. Data of all biochemical parameters of two groups were statistically analyzed by SPSS software, t test, and GraphPad Prism. Concentrations of MDA, TOS, and OSI in tumor tissue were significantly higher than tumor margin tissue, but the level of TAC and CAT, SOD, and GPX activities was significantly reduced in tumor tissue (p<0.05). It was found that the concentrations of Zn and Cu in breast cancer patients were higher than tumor margin tissue. Patients with breast cancer have a rise in oxidative stress indicators and a decrease in antioxidant stress markers. Since oxidative stress is a significant contributor to the development and progression of breast cancer, more research might lead to a more effective method of breast cancer treatment. Considering the dual role of oxidative stress in cancer, which can both cause survival and adaptation, and the death of cancer cells, and with more information, it can be used to manage the treatment and destruction of cancer cells.

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Acknowledgements

The present study has been extracted from a PhD dissertation (project no. 140002281458) and was approved and funded by Hamadan University of Medical Sciences, Hamadan, Iran

Funding

This project was supported by the Deputy of Research and Technology of Hamadan University of Medical Sciences (No 140002281458).

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Authors and Affiliations

  1. Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Zeinab Barartabar, Roghayeh Abbasalipourkabir & Nasrin Ziamajidi

  2. Breast Cancer Research Center, Motamed Cancer Institute, ACERCR, Tehran, Iran

    Nazi Moini

  3. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Seyed Alireza Mesbah-Namin

  4. Molecular Medicine Research Center, Hamadan University of Medical Science, Hamadan, Iran

    Nasrin Ziamajidi

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  1. Zeinab Barartabar
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  2. Nazi Moini
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  3. Roghayeh Abbasalipourkabir
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  4. Seyed Alireza Mesbah-Namin
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  5. Nasrin Ziamajidi
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Contributions

Z.B. did all the experiments and wrote the manuscript, N.M. confirmed patients and gave samples, R.A. analyzed and interpreted the data, S.A.M-N revised the manuscript, and N.Z. generated and developed the study hypothesis and design and completed the final version of the manuscript.

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Correspondence to Nasrin Ziamajidi.

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Barartabar, Z., Moini, N., Abbasalipourkabir, R. et al. Assessment of Tissue Oxidative Stress, Antioxidant Parameters, and Zinc and Copper Levels in Patients with Breast Cancer. Biol Trace Elem Res 201, 3233–3244 (2023). https://doi.org/10.1007/s12011-022-03439-5

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