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Possible Curative Effects of Boric Acid and Bacillus clausii Treatments on TNBS-Induced Ulcerative Colitis in Rats

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Abstract

Crohn’s disease (CD) and ulcerative colitis (UC) are two chronic relapsing inflammatory bowel diseases (IBD). Although there are several treatment options available to improve the symptoms of IBD patients, there is no effective treatment that provides a definitive solution. In the present study, we aim to investigate the antioxidative/anti-inflammatory effects of oral administration of boric acid and Bacillus clausii in a rat trinitrobenzenesulfonic acid (TNBS)-induced colitis model. The effects of boric acid and B. clausii were examined in serum and colon tissues with the help of some biochemical and histological analyses. Elevated inflammation and oxidative damage were found in the blood and colon tissue samples in the TNBS-induced group according to the complete blood count (CBC), tumor necrosis factor (TNF) alpha, interleukin-35 (IL-35), malondialdehyde (MDA), glutathione peroxidase (GPx), myeloperoxidase (MPO), nitric oxide (NO), and histological findings. Particularly, the highest IL-35 level (70.09 ± 12.62 ng/mL) in the combined treatment group, highest catalase activity (5322 ± 668.1 U/mg protein) in the TNBS-induced group, and lower relative expression of inducible nitric oxide synthase in the TNBS-induced group than the control group were striking findings. According to our results, it can be concluded that boric acid showed more curative effects, even if B. clausii probiotics was partially ameliorative.

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Acknowledgements

The authors are thankful to the Director and workers of the Medical and Surgical Experimental Research Centre of the University for their kind cooperation throughout the animal care.

Funding

This project was financially supported by the Scientific Research Projects Commission of the Eskisehir Osmangazi University (Eskişehir, Turkey) (Project #202011D03).

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Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, Eastern Mediterranean University, Famagusta, Cyprus

    Mete Özkoç

  2. Department of Medical Biochemistry, Faculty of Medicine, Eskisehir Osmangazi University, Eskişehir, Turkey

    Betül Can & Güngör Kanbak

  3. Department of Biology, Faculty of Science and Letters, Eskisehir Osmangazi University, Eskişehir, Turkey

    Hakan Şentürk

  4. Department of Histology and Embryology, Faculty of Medicine, Eskisehir Osmangazi University, Eskişehir, Turkey

    Dilek Burukoğlu Dönmez

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  1. Mete Özkoç
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Contributions

Özkoç M. and Kanbak G. designed the study; Özkoç M., Can B., and Şentürk H. performed surgical operations; Özkoç M. and Can B. performed the biochemical experiments, acquired and analyzed data; Özkoç M. and Kanbak G. interpreted the biochemical data; Dönmez Burukoğlu D. carried out the histological experiments and interpreted the histologic data; Özkoç M. wrote the manuscript; all authors read and approved the final manuscript.

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Correspondence to Mete Özkoç.

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All animal research protocols in this study were approved by the Institutional Ethics Committee (HADYEK, Protocol # 778/2019).

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Özkoç, M., Can, B., Şentürk, H. et al. Possible Curative Effects of Boric Acid and Bacillus clausii Treatments on TNBS-Induced Ulcerative Colitis in Rats. Biol Trace Elem Res 201, 1237–1251 (2023). https://doi.org/10.1007/s12011-022-03215-5

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