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Protective Effect of Calcium Fructoborate Against Carbon Tetrachloride–Induced Toxicity in Rats

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Abstract

Carbon tetrachloride (CCl4) is a xenbiotic that can cause cellular damage with free radical production. Calcium fructoborate (CFB) is a boron-based nutritional supplement with antioxidant properties. Calcium fructoborate used in our study is marketed by Future Ceutical Corporation as FruiteX-B, which has a chemical structure similar to the natural form of boron found in edible plants. In this study, it was aimed to determine the antioxidant activity, DNA damage, and histopathological effects of CFB on the liver and kidney tissues of rats in the toxicity induced by CCl4. During 14 days of treatment, 42 wistar albino rats were divided into 7 in each group, control group, olive oil (0.25 ml twice a week), CFB (1 mg/day), CFB-CCl4 (1 mg/day, twice a week 0.5 ml), ZY-CFB (0.25 ml/twice a week, 1 mg/2 times day twice), and CCl4 (0.5 ml twice a week). AST, ALT, HDL, LDH, urea, creatinine, triglyceride, total protein and albumin levels were analyzed in the blood serum of rats. The antioxidant defense system enzymes CAT, GR, GPx, SOD activities and GSH, MDA and 8-OHdG levels in liver and kidney tissues were determined and evaluated. In addition, liver and kidney tissues were examined with only hispatological tests. As a result of the findings, it shows that CCl4 disrupts antioxidant defense mechanisms by disrupting some enzyme systems in the kidney and liver. CFB (Fruit-XB), a boronbased dietary supplement, regulates antioxidant metabolism by strengthening biochemical metabolic profiles against oxidation, and also has a protective effect against DNA damage caused by oxidation. Thus, it was concluded that CFB has antioxidant property against CCl4-induced liver and kidney toxicity.

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Funding

This study was supported by the Van YYU Scientific Research Projects Directorate as the project numbered TKD-2018–7179.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Van Yüzüncü Yıl University, Van, Turkey

    Hatice Aysal & Nurhayat Atasoy

  2. Van Vocational Higher School of Healthcare Studies, Van Yüzüncü Yıl University, Van, Turkey

    Ahmet Ufuk Kömüroğlu

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  1. Hatice Aysal
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  2. Nurhayat Atasoy
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Correspondence to Nurhayat Atasoy.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted (Van Yüzüncü Yıl University Experimental Animal Unit., protocol no: 2018/04).

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Aysal, H., Atasoy, N. & Kömüroğlu, A.U. Protective Effect of Calcium Fructoborate Against Carbon Tetrachloride–Induced Toxicity in Rats. Biol Trace Elem Res 201, 800–809 (2023). https://doi.org/10.1007/s12011-022-03202-w

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