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Use of Microscopic Characteristics and Multielemental Fingerprinting Analysis to Trace Three Different Cultivation Modes of Medicinal and Edible Dendrobium officinale in China

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Abstract

The traceability of different cultivation modes is critical for ensuring the commercial viability of high-value Dendrobium officinale. In this study, by means of polarizing microscopy, SEM–EDX, ICP-MS and ICP-AES, the possibility of combining microscopic characteristics, multielemental analysis and multivariate statistical authenticity analysis was realized to determine the origins of the fresh stem and dried stem powder of D. officinale derived from three different cultivation modes from six provinces of China. The microscopic structure, chemical elements on the surface of the main microstructures and concentrations of Ca, K, Ba, Cs, As and Cu varied among specimens derived from different cultivation modes. The fresh stems of D. officinale derived from different cultivation modes can be effectively and quickly identified by various microscopic characteristics and different contents of Ca on the surface of the parenchyma, phloem and xylem. Meanwhile, linear discriminant analysis showed that 98.1% of the dried stem powder samples were correctly classified, and the accuracy of cross-validation was 95.3%. This study facilitated an effective integrated method for determining the traceability of the fresh stem and dried stem powder of D. officinale derived from three different cultivation modes. This approach offers a potential method for identifying the origins of medicinal plants derived from different cultivation modes.

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All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Acknowledgements

The authors would like to thank the State Key Laboratory of Environment, Institute of Geochemistry, Chinese Academy of Sciences (Guiyang), for providing the instrumentation.

Funding

This research was financially supported by the National Natural Science Foundation of China (Project No. 82160717), the Science and Technology planning project of Guizhou Province (Project Nos. [2020]4Y073, [2019]3001–1, and [2019]4329), and Doctoral startup fund of Guizhou University of traditional Chinese medicine (Project No. [2019] No. 067).

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Authors and Affiliations

  1. Guizhou University of Traditional Chinese Medicine, Dongqing South Road, Huaxi, Guiyang, 550025, GuiZhou, China

    Guangying Du, Fulin Yan, Shenghua Wei, Deqiang Ren & Xiangping Li

  2. Guizhou University, Huaxi, Guiyang, 550025, GuiZhou, China

    Ruidong Yang

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  1. Guangying Du
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  2. Ruidong Yang
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  3. Fulin Yan
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Contributions

Guangying Du involved in conceptualization, methodology, investigation, writing—original draft, visualization, funding acquisition. Ruidong Yang took part in writing—review & editing, supervision. Fulin Yan involved in conceptualization and sample collection. Shenghua Wei took part in supervision and project administration. Deqiang Ren took part in revising grammar and funding acquisition. Xiangping Li involved in data curation.

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Correspondence to Guangying Du.

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Du, G., Yang, R., Yan, F. et al. Use of Microscopic Characteristics and Multielemental Fingerprinting Analysis to Trace Three Different Cultivation Modes of Medicinal and Edible Dendrobium officinale in China. Biol Trace Elem Res 201, 1006–1018 (2023). https://doi.org/10.1007/s12011-022-03196-5

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