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The Effects of Vitamin D Application on NaF-Induced Cytotoxicity in Osteoblast Cells (hFOB 1.19)

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Abstract

This study was planned to evaluate the effect of vitamin D administration on cytotoxicity due to fluoride exposure in vitro. NaF (IC50) and vitamin D (proliferative) were applied to human osteoblast (hFOB 1.19) cells. The major genes of apoptotic, autophagic, and necrotic pathways were determined by RT-PCR. 2-∆∆Ct formulation was used for expression analysis. In the NaF group, caspase 3, Bax, Bad, Bak, Bclx, Atg3, Atg5, Atg6, pG2, LC3-I, LC3-II, RIP1, and RIP3 genes were increased (2.6–15 times). It was observed that the expressions of these genes approached the control when vitamin D was given together with NaF. The Bcl2 gene increased significantly (sixfold) with the effect of NaF, and was down-regulated to some extent with additional vitamin D administration, but still more than in the control. As a result, it was determined that apoptotic, necrotic, and autophagic pathways were activated as the molecular basis of the damage in the bone tissue, which was most affected by fluorine, and these genes were down-regulated and approached the control group with the addition of vitamin D. It was concluded that this is an important data to explain the molecular basis of the protective and therapeutic effect of vitamin D against fluorine toxicity.

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Data Availability

The data that support the findings of this study are available from the corresponding author, [Semiha DEDE], upon reasonable request.

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Acknowledgements

This research was carried out by Van Yuzuncu Yil University Scientific Research Projects Coordination Unit as project numbered TSA-2017-5949.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Turkey

    Semiha Dede & Sedat Çetin

  2. Department of Medical Biology, Faculty of Medicine, Aksaray University, Aksaray, Turkey

    Mehmet Taşpinar

  3. Department of Medical Laboratory, Özalp Vocational High School, Van Yuzuncu Yil University, Van, Turkey

    Veysel Yüksek

  4. Department of Chemistry, Faculty of Science, Van Yuzuncu Yil University, Van, Turkey

    Ayşe Usta

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  1. Semiha Dede
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Correspondence to Semiha Dede.

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Dede, S., Taşpinar, M., Yüksek, V. et al. The Effects of Vitamin D Application on NaF-Induced Cytotoxicity in Osteoblast Cells (hFOB 1.19). Biol Trace Elem Res 201, 698–705 (2023). https://doi.org/10.1007/s12011-022-03177-8

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