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Genes and Signaling Pathways Involved in the Regulation of Selenium-Enriched Yeast on Liver Metabolism and Health of Broiler (Gallus gallus)

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Abstract

Selenium-enriched yeast (SeY) plays an important role in the liver health and metabolism of the broiler. However, the mechanism by which it regulates liver metabolism and the health of broilers is largely unknown. Therefore, this study was conducted to elucidate the key genes and signaling pathways involved in regulating SeY in liver metabolism and bird’s health. Thus, the mRNA expression microarray, GSE25151, was downloaded from Gene Expression Omnibus (GEO) database. GSE25151 consists of liver samples from SeY-treated and the control broilers. Six hundred four differentially expressed genes (DEGs) were identified in livers between SeY-treated and control. Gene ontology (GO) enrichment analysis indicated that those DEGs are mainly involved in metabolism-related biological processes, such as biological regulation, molecular processes, responses to stimuli, cell communication and proliferation, and growth. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed the DEGs mainly enriched in metabolism-related signaling pathways, including PI3K, Akt, Wnt, calcium, IGF1 receptor, and MAPK signaling pathways. Moreover, many genes, such as NMUR1, NMU, and GPRC6A, might contribute to the regulation of SeY to broiler liver metabolism and health. In conclusion, the current study enhances our understanding of the regulation of SeY in liver metabolism and health of the birds and will assist studies of the molecular mechanisms of SeY regulation in chicken liver.

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Abbreviations

Se:

Selenium

SeY:

Selenium-enriched yeast

GEO:

Gene Expression Omnibus

DETs:

Differentially expressed transcripts

DEGs:

Differentially expressed genes

GO:

Gene Ontology

KEGG:

Kyoto Encyclopedia of Genes and Genomes

MDA:

Malonaldehyde

IGF1R:

Insulin-like growth factor 1 receptor

GSH:

Glutathione

MMP:

Metalloprotease

ALT:

Alanine aminotransferase

AST:

Aspartate aminotransferase

T-SOD:

Total superoxide dismutase

SOD:

Superoxide dismutase

iNOS:

Inducible nitric oxide synthase

TNOs:

Total nitric oxide synthase

GSH-px:

Glutathione peroxidase

AFB1:

Aflatoxin B1

LCRNA:

Labelled complementary RNA

NCBI:

National Center for Biotechnology Information Support Center database

UMAP:

Uniform Manifold Approximation and Projection

KOBAS:

KEGG Orthology-Based Annotation System

FC:

Fold change

PPI:

Protein–protein interaction

DEHP:

Di-(2-ethylhexyl) phthalate

GM:

Germacrone

HF:

Hepatic fibrosis

HSCs:

Hepatic stellate cells

DXZ:

Doxazosin

NO:

Nitric oxide

Cd:

Cadmium

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Funding

This study was supported by Natural Science Foundation of Anhui Provincial Education Department (No. KJ2020A0083), Talent Introduction Program of Anhui Science and Technology University (No. DKYJ202003), the Open Project of Anhui Province Key Laboratory of Wetland Ecosystem Protection and Restoration (No. AHZDSYS2019-01), Science and Technology Planning Projects of Tongren City (No. 2016–17-4 and 2017–47-91), and the Open Project of Longyan University & Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology (No. ZDSYS2020004).

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Authors and Affiliations

  1. College of Animal Science, Anhui Science and Technology University, Fengyang, 233100, China

    Xiaofeng Li, Jinling Hua, Shujuan Wang, Zhongze Hu, Aiyou Wen & Bing Yang

  2. Anhui Province Key Laboratory of Wetland Ecosystem Protection and Restoration, Anhui University, Hefei, 230039, China

    Bing Yang

  3. Longyan University & Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University, Longyan, 364012, China

    Bing Yang

  4. Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Fengyang, 233100, China

    Bing Yang

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  1. Xiaofeng Li
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  2. Jinling Hua
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  3. Shujuan Wang
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  4. Zhongze Hu
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  5. Aiyou Wen
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  6. Bing Yang
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Contributions

XF Li conceived the study. XF Li and JL Hua analyzed the results, SJ Wang and ZZ Hu prepared the figures and tables. B Yang wrote the manuscript. All the authors reviewed the manuscript.

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Correspondence to Bing Yang.

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Li, X., Hua, J., Wang, S. et al. Genes and Signaling Pathways Involved in the Regulation of Selenium-Enriched Yeast on Liver Metabolism and Health of Broiler (Gallus gallus). Biol Trace Elem Res 201, 387–402 (2023). https://doi.org/10.1007/s12011-022-03150-5

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