Skip to main content

Advertisement

SpringerLink
Log in

Therapeutic Effects of Boric Acid in a Septic Arthritis Model Induced by Escherichia coli in Rats

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

A Correction to this article was published on 07 February 2022

This article has been updated

Abstract

The study aimed to evaluate the therapeutic effect of boric acid (BA) in experimentally induced septic arthritis. A total of 30 rats, 6 rats in each group (5 groups), were used in the study. No treatment was applied to the rats in the control group. Only BA was administered intraperitoneally (IP) to the rats in the bor group. Escherichia coli was administered at a single dose of 25 μL, 1 × 1010 cfu/rat from the right foot pad of the rats, via intra-articular route, to the mice in the arthritis, arthritis-bor, and arthritis-antb groups. Then, BA at a dose of 50 mg/kg and cefazolin at a dose of 25 mg/kg were administered to the rats in the arthritis-bor and arthritis-antb groups, respectively, for 7 days via the IP route. At the end of the study, all animals were euthanized following the ethical rules. Blood and tissue samples were taken from the rats for biochemical and histopathological analyses. The levels of GSH, MDA, Endoglin, Endocan, and TNF-β markers were measured in the blood samples taken. A significant decrease was observed in MDA and Endoglin levels in the boric acid–administered group compared with the arthritis group, while a significant increase was observed at the GSH level. Histopathologically, it was determined that the reactive surrounding tissue response in the bor group was significantly reduced. As a result, a significant decrease in inflammation was found biochemically and histopathologically in the groups treated with BA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Change history

References

  1. Gottlieb M, Holladay D, Rice M (2019) Current approach to the evaluation and management of septic arthritis. Pediatr Emerg Care 35:509–513

    Article  PubMed  Google Scholar 

  2. Hassan AS, Rao A, Manadan AM, Block JA (2017) Peripheral bacterial septic arthritis: review of diagnosis and management. JCR J Clin Rheumatol 23:435–442

    Article  PubMed  Google Scholar 

  3. Kromann NC, Johansen ME, Hvolris J (2017) Septic arthritis. Ugeskr Laeger 179(3):2–6

    Google Scholar 

  4. Agarwal A, Aggarwal AN (2016) Bone and joint infections in children: septic arthritis. Indian J Pediatr 83:825–833

    Article  PubMed  Google Scholar 

  5. Brown DW, Sheffer BW (2019) Pediatric septic arthritis: an update. Orthop Clin 50:461–470

    Google Scholar 

  6. Wall C, Donnan L (2015) Septic arthritis in children. Aust Fam Physician 44:213–215

    PubMed  Google Scholar 

  7. Cohen E, Katz T, Rahamim E et al (2020) Septic arthritis in children: Updated epidemiologic, microbiologic, clinical and therapeutic correlations. Pediatr Neonatol 61:325–330

    Article  PubMed  Google Scholar 

  8. NI Montgomery HR Epps 2017 Pediatric septic arthritis OrthopClin 48 209 216

  9. Carpenter CR, Schuur JD, Everett WW, Pines JM (2011) Evidence-based diagnostics: adult septic arthritis. Acad Emerg Med 18:781–796

    Article  PubMed  PubMed Central  Google Scholar 

  10. García-Arias M, Balsa A, Mola EM (2011) Septic arthritis. Best Pract Res Clin Rheumatol 25:407–421. https://doi.org/10.1016/j.berh.2011.02.001

    Article  PubMed  Google Scholar 

  11. Mathew AJ, Ravindran V (2014) Infections and arthritis. Best Pract Res Clin Rheumatol 28:935–959

    Article  PubMed  Google Scholar 

  12. Johnson JR, Gajewski A, Lesse AJ, Russo TA (2003) Extraintestinal pathogenic Escherichia coli as a cause of invasive nonurinary infections. J Clin Microbiol 41:5798

    Article  PubMed  PubMed Central  Google Scholar 

  13. Wasiński B (2019) Extra-intestinal pathogenic Escherichia coli–threat connected with food-borne infections. Ann Agric Environ Med 26:532–537

    Article  PubMed  CAS  Google Scholar 

  14. Gaußmann B, Hess C, Grafl B et al (2018) Escherichia coli isolates from femoral bone marrow of broilers exhibit diverse pheno-and genotypic characteristics that do not correlate with macroscopic lesions of bacterial chondronecrosis with osteomyelitis. Avian Pathol 47:271–280

    Article  PubMed  CAS  Google Scholar 

  15. Hamzaoui A, Salem R, Koubaa M et al (2009) Escherichia coli osteomyelitis of the ischium in an adult. Orthop Traumatol Surg Res 95:636–638

    Article  PubMed  Google Scholar 

  16. Marcos Sánchez F, Aragón Díez A, Arbol Linde F, et al (2004) [Vertebral osteomyelitis caused by E. coli]. An Med Interna Madr Spain 1984 21 43 44.

  17. Matsuura H, Sue M, Takahara M, Kuninaga N (2019) Escherichia coli rib osteomyelitis. QJM 112:35–36

    Article  CAS  PubMed  Google Scholar 

  18. Moustafa A, Kheireldine R, Khan Z, et al (2019) Cervical spinal osteomyelitis with epidural abscess following an Escherichia coli urinary tract infection in an immunocompetent host. Case Rep Infect Dis 2019:

  19. Xie Z, Cui X, Zhao C et al (2013) Gentamicin-loaded borate bioactive glass eradicates osteomyelitis due to Escherichia coli in a rabbit model. Antimicrob Agents Chemother 57:3293

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Braga JFV, Chanteloup NK, Trotereau A et al (2016) Diversity of Escherichia coli strains involved in vertebral osteomyelitis and arthritis in broilers in Brazil. BMC Vet Res 12:1–12

    Article  CAS  Google Scholar 

  21. Mathews CJ, Weston VC, Jones A et al (2010) Bacterial septic arthritis in adults. The Lancet 375:846–855

    Article  Google Scholar 

  22. Acaroz U, Ince S, Arslan-Acaroz D et al (2018) The ameliorative effects of boron against acrylamide-induced oxidative stress, inflammatory response, and metabolic changes in rats. Food Chem Toxicol 118:745–752

    Article  CAS  PubMed  Google Scholar 

  23. Arslan-Acaroz D, Bayşu-Sozbilir N (2020) Ameliorative effect of boric acid against formaldehyde-induced oxidative stress in A549 cell lines. Environ Sci Pollut Res 27:4067–4074

    Article  CAS  Google Scholar 

  24. Ince S, Kucukkurt I, Acaroz U, et al (2019) Boron ameliorates arsenic-induced DNA damage, proinflammatory cytokine gene expressions, oxidant/antioxidant status, and biochemical parameters in rats. J Biochem Mol Toxicol 33:e22252

  25. Nielsen FH (2014) Update on human health effects of boron. J Trace Elem Med Biol 28:383–387

    Article  CAS  PubMed  Google Scholar 

  26. Brittingham A, Wilson WA (2014) The antimicrobial effect of boric acid on Trichomonas vaginalis. Sex Transm Dis 41:718–722

    Article  CAS  PubMed  Google Scholar 

  27. Haesebrouck F, Baele M, De Keyser H et al (2009) Antimicrobial activity of an acetic and boric acid solution against Staphylococcus pseudintermedius. Vlaams Diergeneeskd Tijdschr 78:89–90

    Google Scholar 

  28. Beutler E, Duron O, Kelly B (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882–888

    CAS  PubMed  Google Scholar 

  29. Yoshioka T, Kawada K, Shimada T, Mori M (1979) Lipid peroxidation in maternal and cord blood and protective mechanisms. Am J Obstet Gynecol 135:972–976

    Article  Google Scholar 

  30. Castellazzi L, Mantero M, Esposito S (2016) Update on the management of pediatric acute osteomyelitis and septic arthritis. Int J Mol Sci 17:855

    Article  PubMed Central  CAS  Google Scholar 

  31. Arnold JC, Bradley JS (2015) Osteoarticular infections in children. Infect Dis Clin 29:557–574

    Article  Google Scholar 

  32. Mellata M (2013) Human and avian extraintestinal pathogenic Escherichia coli: infections, zoonotic risks, and antibiotic resistance trends. Foodborne Pathog Dis 10:916–932

    Article  PubMed  PubMed Central  Google Scholar 

  33. Ergönül Ö, Aydin M, Azap A et al (2016) Healthcare-associated Gram-negative bloodstream infections: antibiotic resistance and predictors of mortality. J Hosp Infect 94:381–385

    Article  PubMed  Google Scholar 

  34. Lefebvre M-A, Quach C, Daniel SJ (2015) Chronic suppurative otitis media due to nontuberculous mycobacteria: a case of successful treatment with topical boric acid. Int J Pediatr Otorhinolaryngol 79:1158–1160

    Article  PubMed  Google Scholar 

  35. Schmidt M, Schaumberg JZ, Steen CM, Boyer MP (2010) Boric acid disturbs cell wall synthesis in Saccharomyces cerevisiae. Int J Microbiol 2010:930465

  36. Güzel Y, Golge UH, Goksel F et al (2016) The efficacy of boric acid used to treat experimental osteomyelitis caused by methicillin-resistant Staphylococcus aureus: an in vivo study. Biol Trace Elem Res 173:384–389

    Article  PubMed  CAS  Google Scholar 

  37. Saito T, Bokhove M, Croci R et al (2017) Structural basis of the human endoglin-BMP9 interaction: insights into BMP signaling and HHT1. Cell Rep 19:1917–1928

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Altomonte M, Montagner R, Fonsatti E et al (1996) Expression and structural features of endoglin (CD105), a transforming growth factor β 1 and β 3 binding protein, in human melanoma. Br J Cancer 74:1586–1591

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Barbara NP, Wrana JL, Letarte M (1999) Endoglin is an accessory protein that interacts with the signaling receptor complex of multiple members of the transforming growth factor-β superfamily. J Biol Chem 274:584–594

    Article  CAS  PubMed  Google Scholar 

  40. Lassalle P, Molet S, Janin A et al (1996) ESM-1 is a novel human endothelial cell-specific molecule expressed in lung and regulated by cytokines. J Biol Chem 271:20458–20464

    Article  CAS  PubMed  Google Scholar 

  41. Scherpereel A, Depontieu F, Grigoriu B et al (2006) Endocan, a new endothelial marker in human sepsis. Crit Care Med 34:532–537

    Article  CAS  PubMed  Google Scholar 

  42. Kali A, Shetty KR (2014) Endocan: a novel circulating proteoglycan. Indian J Pharmacol 46:579

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Takaoka Y, Abe Y, Haraguchi R, Kito K (2010) Lymphotoxin (TNF-beta). Nihon Rinsho Jpn J Clin Med 68:93–95

    Google Scholar 

  44. Calmon-Hamaty F, Combe B, Hahne M, Morel J (2011) Lymphotoxin α revisited: general features and implications in rheumatoid arthritis. Arthritis Res Ther 13:1–5

    Article  CAS  Google Scholar 

  45. Ruddle NH (2014) Lymphotoxin and TNF: how it all began—a tribute to the travelers. Cytokine Growth Factor Rev 25:83–89

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Aggarwal BB, Gupta SC, Kim JH (2012) Historical perspectives on tumor necrosis factor and its superfamily: 25 years later, a golden journey. Blood J Am Soc Hematol 119:651–665

    CAS  Google Scholar 

  47. Buch MH, Conaghan PG, Quinn MA et al (2004) True infliximab resistance in rheumatoid arthritis: a role for lymphotoxin α? Ann Rheum Dis 63:1344–1346

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. O’Rourke KP, O’Donoghue G, Adams C et al (2008) High levels of Lymphotoxin-Beta (LT-Beta) gene expression in rheumatoid arthritis synovium: clinical and cytokine correlations. Rheumatol Int 28:979–986

    Article  PubMed  CAS  Google Scholar 

  49. Chiang EY, Kolumam GA, Yu X et al (2009) Targeted depletion of lymphotoxin-α–expressing TH 1 and TH 17 cells inhibits autoimmune disease. Nat Med 15:766–773

    Article  CAS  PubMed  Google Scholar 

  50. Guis S, Balandraud N, Bouvenot J et al (2007) Influence of- 308 A/G polymorphism in the tumor necrosis factor α gene on etanercept treatment in rheumatoid arthritis. Arthritis Care Res 57:1426–1430

    Article  CAS  Google Scholar 

  51. Mugnier B, Balandraud N, Darque A et al (2003) Polymorphism at position- 308 of the tumor necrosis factor α gene influences outcome of infliximab therapy in rheumatoid arthritis. Arthritis Rheum Off J Am Coll Rheumatol 48:1849–1852

    Article  CAS  Google Scholar 

  52. Seitz M, Wirthmüller U, Möller B, Villiger P (2007) The- 308 tumour necrosis factor-α gene polymorphism predicts therapeutic response to TNFα-blockers in rheumatoid arthritis and spondyloarthritis patients. Rheumatology 46:93–96

    Article  CAS  PubMed  Google Scholar 

  53. Eroğlu HA, Makav M, Adali Y, Citil M (2020) Effects of ozone and L-carnitine on kidney MDA, GSH, and GSHPx levels in acetaminophen toxicity. Kafkas Üniversitesi Vet Fakültesi Derg 26(1):127–134

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Orthopeadia and Traumatology Department, Kafkas University Medical School, Ana Kampüs, 36000, Kars, Turkey

    Kadri Yıldız

  2. Physiology Department, Kafkas University Veterinary School, Kars, Turkey

    Mustafa Makav

  3. Department of Pathology, Faculty of Medicine, Izmir University of Economics, İzmir, Turkey

    Yasemen Adalı

  4. Food Engineering Department, Faculty of Engineering, Igdır University, Igdır, Turkey

    Menekşe Bulut

Authors
  1. Kadri Yıldız
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mustafa Makav
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yasemen Adalı
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Menekşe Bulut
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kadri Yıldız.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yıldız, K., Makav, M., Adalı, Y. et al. Therapeutic Effects of Boric Acid in a Septic Arthritis Model Induced by Escherichia coli in Rats. Biol Trace Elem Res 200, 4762–4770 (2022). https://doi.org/10.1007/s12011-021-03065-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-021-03065-7

Keywords

Search

Navigation