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Dietary Effect of Probiotic and Prebiotic on Some Mucus Indices of Silver Carp (Hypophthalmichthys molitrix) Exposed to Silver Nanoparticles

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A Correction to this article was published on 11 April 2024

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Abstract

Prebiotic and probiotic supplements can directly activate fish primary defense mechanisms by acting on responsible receptors. This study aimed to investigate the effect of using Pediococcus acidilactici and Agaricus bisporus separately and in combination with silver carp’s mucosal immune system indices and finally the possibility of reducing the toxicity of silver nanoparticles in silver carp. After calculating LC50, the sub-lethal toxicity test was performed to sub-lethal concentration of silver nanoparticles for 14 days. Fish skin mucus was collected in polyethylene bags containing 10 ml of 50 mM sodium chloride for future analysis. Results of skin mucosal indices activity showed significant changes in treatments fed with additives compared to the control treatment after exposure to silver nanoparticles on days 1, 7, and 14 in including lysozyme enzyme activity, total protein, and skin mucosal protein pattern. The addition of additives will improve the relative mucosal safety indices of silver carp. The results of statistical analysis showed that the addition of combination supplements, Pediococous acidilactici, and Agaricus bisporus powder could affect the skin mucosal safety indices. It is effective and ultimately improves the immune system function of silver carp skin in 14-day exposure to silver nanoparticles.

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References

  1. Baba E, Uluköy G, Öntaş C (2015) Effects of feed supplemented with Lentinula edodes mushroom extract on the immune response of rainbow trout, Oncorhynchus mykiss, and disease resistance against Lactococcus garvieae. Aquaculture 448:476–482

    Article  CAS  Google Scholar 

  2. Benn T, Cavanagh B, Hristovski K, Posner JD, Westerhoff P (2010) The release of nanosilver from consumer products used in the home. J Environm Qual 39(6):1875–1882

    Article  CAS  Google Scholar 

  3. Bricknell I, Dalmo RA (2005) The use of immunostimulants in fish larval aquaculture. Fish Shellfish Immunol 19:457–472

    Article  CAS  PubMed  Google Scholar 

  4. Castex M, Lemaire P, Wabete N, Chim L (2010) Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge. Fish Shellfish Immunol 28(4):622–631

    Article  CAS  PubMed  Google Scholar 

  5. Di Giulio RT, Hinton DE (2008) The toxicology of fishes. Taylor Francis. 319–884.dietary inulin and oligosaccharides as prebiotics for weaning Turbot (Psetta maxima). Aquacult Int 14:219–229

    Google Scholar 

  6. Din ARJM, Razak SA, Sabaratnam V (2012) Effect of mushroom supplementation as a prebiotic compound in super worm based diet on growth performance of red tilapia fingerlings. Sains Malaysiana 41(10):1197–1203

    CAS  Google Scholar 

  7. Dongarrà ML, Rizzello V, Muccio L, Fries W, Cascio A, Bonaccorsi I, Ferlazzo G (2013) Mucosal immunology and probiotics. Curr Allergy Asthma Rep 13:19–26

    Article  PubMed  Google Scholar 

  8. Garcia-Reyero N, Thornton C, Hawkins AD, Escalon L, Kennedy AJ, Steevens JA, Willett KL (2015) Assessing the exposure to nanosilver and silver nitrate on fathead minnow gill gene expression and mucus production. Environ Nanotechnol Monit Manag 4:58–66

    Google Scholar 

  9. Gatesoupe FJ (1999) The use of probiotics in aquaculture. Aquaculture 180(1):147–165

    Article  Google Scholar 

  10. Griffitt RJ, Luo J, Gao J, Bonzongo JC, Barber DS (2008) Effects of particle composition and species on toxicity of metallic nanomaterials in aquatic organisms. Environ Toxicol Chem 27(9):1972–1978

    Article  CAS  PubMed  Google Scholar 

  11. Guardiola FA, Dioguardi M, Parisi MG, Trapani MR, Meseguer J, Cuesta A, Esteban MA (2015) Evaluation of waterborne exposure to heavy metals in innate immune defences present on skin mucus of gilthead seabream (Sparus aurata). Fish Shellfish Immunol 45:112–123

    Article  CAS  PubMed  Google Scholar 

  12. Harikrishnan R, Balasundaram C, Heo MS (2012) Inonotus obliquus containing diet enhances the innate immune mechanism and disease resistance in olive flounder Paralichythys olivaceus against Uronema marinum. Fish Shellfish Immunol 32(6):1148–1154

    Article  CAS  PubMed  Google Scholar 

  13. Hedayati A, Farsani H, Naserabad S, Hoseinifar S. Van, Doan H (2019) Protective effect of dietary vitamin E on immunological and biochemical induction through silver nanoparticles (AgNPs) inclusion in diet and silver salt (AgNO3) exposure on Zebrafish. Comp Biochem Physiol C 222:100–107

    CAS  Google Scholar 

  14. Hedayati A (2016) Effect of 2- phenoxyethanol anesthesia on some haematological and biochemical indices of silver carp. Iran J Fish Sci 17(1):1–10

    Google Scholar 

  15. Hernandez LHH, Barrera TC, Mejia JC, Mejia GC, Del Carmen M, Dosta M, De Lara Andrade R, Sotres JAM (2010) Effects of the commercial probiotic Lactobacillus casei on the growth, protein content of skin mucus and stress resistance of juveniles of the Porthole livebearer Poecilopsis gracilis (Poecilidae). Aquacult Nutr 16(4):407–411

    Article  CAS  Google Scholar 

  16. Hoseinifar SH, Mirvaghefi A, Amoozegar MA, Sharifian M, Esteban MÁ (2015) Modulation of innate immune response, mucosal parameters and disease resistance in rainbow trout (Oncorhynchus mykiss) upon synbiotic feeding. Fish Shellfish Immunol 45(1):27–32

    Article  CAS  PubMed  Google Scholar 

  17. Keller AA, McFerran S, Lazareva A, Suh S (2013) Global life cycle releases of engineered nanomaterials. J Nanoparticle Res 15(6):1692

    Article  Google Scholar 

  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1):265–75

    Article  CAS  PubMed  Google Scholar 

  19. Luoma SN (2008) Silver nanotechnologies and the environment: old problems and new challenges. The Project on Emerging Nanotechnologies Report, 15. Washington DC: Woodrow Wilson International Center for Scholars or The PEW Charitable Trusts.

  20. Magnadottir B (2006) Innate immunity of fish (overview). Fish Shellfish Immunol 20:137–151

    Article  CAS  PubMed  Google Scholar 

  21. Miandare HK, Yarahmadi P, Abbasian M (2016) Immune related transcriptional responses and performance of Litopenaeus vannamei post-larvae fed on dietary probiotic PrimaLac®. Fish Shellfish Immunol 55:671–678

    Article  CAS  PubMed  Google Scholar 

  22. Navarro E, Piccapietra F, Wagner B, Marconi F, Kaegi R, Odzak N (2008) Toxicity of silver nanoparticles to Chlamydomonas reinhardtii. Environ Sci Technol 42:8959–8964

    Article  CAS  PubMed  Google Scholar 

  23. Raj VS, Fournier G, Rakus K, Ronsmans M, Ouyang P, Michel B (2011) Skin mucus of Cyprinus carpio inhibits cyprinid herpesvirus 3 binding to epidermal cells. Vet Res 1:42–92

    Google Scholar 

  24. Sawosz E, Grodzik M, Zielińska M, Niemiec T, Olszańska B, Chwalibog A (2009) Nanoparticles of silver do not affect growth, development and DNA oxidative damage in chicken embryos. Arch Geflügelkunde 73(3):208–213

    CAS  Google Scholar 

  25. Shahare B, Yashpal M, Singh G (2013) Toxic effects of repeated oral exposure of silver nanoparticles on small intestine mucosa of mice. Toxicol Mech Methods 23(3):161–167

    Article  CAS  PubMed  Google Scholar 

  26. Sheikhzadeh N, Heidarieh M, Karimi Pashaki A, Nofouzi K, Ahrab Farshbafi M, Akbari M (2012) Hilyses, Fermented Saccharomyces cerevisiae, enhances the growth performance and skin non-specific immune parameters in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol 32:407–410

    Article  CAS  PubMed  Google Scholar 

  27. Soleimani N, Hoseinifar SH, Merrifield DL, Barati M, Abadi ZH (2012) Dietary supplementation of fructooligosaccharide (FOS) improves the innate immune response, stress resistance, digestive enzyme activities and growth performance of Caspian roach (Rutilus rutilus) fry. Fish Shellfish Immunol 32(2):316–321

    Article  CAS  PubMed  Google Scholar 

  28. Subramanian S, MacKinnon ShL, Ross NW (2007) A comparative study on innate immune parameters in the epidermal mucus of various fish species. Comp Biochem Physiol 148:256–263

    Article  Google Scholar 

  29. Veisi RS, Hedayati A, Mazandarani M, Nodeh AJ, Bagheri T (2021) Dietary beet molasses enhanced immunity system of common carp exposed to copper nano oxide, CuO-NP. Aquac Rep 19:100589

    Article  Google Scholar 

  30. Wasser SP (2002) Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol 60(3):258–274

    Article  CAS  PubMed  Google Scholar 

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  1. Department of Fisheries and Aquatic Sciences, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

    Paria Houshmand & Aliakbar Hedayati

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  1. Paria Houshmand
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  2. Aliakbar Hedayati
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Correspondence to Aliakbar Hedayati.

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Houshmand, P., Hedayati, A. Dietary Effect of Probiotic and Prebiotic on Some Mucus Indices of Silver Carp (Hypophthalmichthys molitrix) Exposed to Silver Nanoparticles. Biol Trace Elem Res 200, 4828–4836 (2022). https://doi.org/10.1007/s12011-021-03047-9

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