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Protective Effect of Manganese on Apoptosis and Mitochondrial Function of Heat-Stressed Primary Chick Embryonic Myocardial Cells

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Abstract

Heat stress, as a kind of oxidative stress, induces cell apoptosis. Apoptosis is a form of programmed cell death, and mitochondria play an important role in apoptosis. Manganese (Mn) has an antioxidant capacity by enhancing the activity of manganese superoxide dismutase (MnSOD). To investigate the potential effect of Mn on heat stress-induced apoptosis and mitochondrial function, we examined crucial related factors in the context of heat stress using primary chick embryonic myocardial cells pretreated with Mn for 24 h. The results showed that Mn restored the heat stress-induced decrease in cell viability and reduced the activities of caspase-3 (P < 0.05). The repression of the Δψm and intracellular ATP content caused by heat stress was reversed dramatically in the Mn pretreatment group (P < 0.05). Additionally, Mn inhibited heat stress-induced mitochondrial fission, as shown by decreased mitochondrial fission-related protein dynamin-related protein 1 (Drp1) expression and increased mitochondrial fusion-related protein optic atrophy 1 (Opa1) and mitofusin 1 (Mfn1) (P < 0.05) in primary chick embryonic myocardial cells. It was concluded that Mn attenuates the mitochondrial-mediated apoptosis pathway and sustains mitochondrial structure and function under heat stress in primary chick embryonic myocardial cells.

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Funding

This research was funded by National Natural Science Foundation of China (No. 03119023).

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Author notes

  1. Shizhen Qin and Yanli Guo contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, People’s Republic of China

    Rui Wang, Zhaoguo Shi, Jinlu Li, Defu Tang, Shizhen Qin & Yanli Guo

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  1. Rui Wang
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  2. Zhaoguo Shi
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  3. Jinlu Li
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Contributions

Rui Wang: design, investigation and writing-original draft; Zhaoguo Shi: project administration; Jinlu Li and Defu Tang: contributed materials; Yanli Guo: conceptualization and writing (review and editing); Shizhen Qin: conceptualization and funding acquisition.

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Correspondence to Shizhen Qin.

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All procedures were approved by the Animal Welfare Committee of the Institute of Animal Science, Gansu Agricultural University.

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Wang, R., Shi, Z., Li, J. et al. Protective Effect of Manganese on Apoptosis and Mitochondrial Function of Heat-Stressed Primary Chick Embryonic Myocardial Cells. Biol Trace Elem Res 200, 4419–4429 (2022). https://doi.org/10.1007/s12011-021-03016-2

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