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Association of TRPM5 Asn235Ser Polymorphism and Trace Elements/Minerals in Chronic Gastritis Patients: a Case-Control Study

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Abstract

The link between chronic gastritis and chemosensory receptors is considered promising for disease prediction and treatment. The transient receptor potential melastatin member channel 5 (TRPM5) is an ion channel and may be a chemosensor in the gastrointestinal tract. Trace elements and minerals involved in many protein structures can change ion channel activity. Our study aimed to determine trace element and mineral levels according to TRPM5 Asn235Ser missense polymorphism distributions in patients with H. pylori (+) and (-) gastritis and to uncover their possible association with disease pathogenesis. In 109 volunteer patients diagnosed with gastritis [64 H. pylori (+), 45 H. pylori (-)], TRPM5 (Asn235Ser) polymorphism was detected by Kompetitive Allele-Specific PCR method (KASP), but no difference was found between the groups. There are differences between the serum trace element (Se, Zn, Cu, Mg, and Cu/Zn) levels of the two groups. High serum Se and Cu/Zn ratios were detected in H. pylori (+) patients. Cu/Zn ratio can be used as a marker of bacterial inflammation. The amount of Se was found to be statistically significant in the serums of H. pylori (+) patients carrying TT and CT genotype, while the amounts of Zn, Cu, and Mg were found as significant of H. pylori (-) patients. The relationship of Se with the H. pylori needs to be investigated further. In addition, H. pylori (+) patients have a significantly higher neutrophils/lymphocytes ratio (NLR) compared to those with H. pylori (-) NLR can be used as an indicator of inflammation.

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Authors and Affiliations

  1. Department of Medical Biology, Faculty of Medicine, Istanbul Aydin University, Istanbul, Turkey

    G. Koc & A. Soyocak

  2. Department of Biophysics, Faculty of Medicine, Istanbul Aydin University, Istanbul, Turkey

    D. Duzgun Ergun

  3. Department of Biophysics, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey

    N. Pastaci Ozsobaci

  4. Department of Nutrition and Dietetic, Health Science Faculty, Istanbul Zaim University, Istanbul, Turkey

    S. Andac-Ozturk

  5. Department of General Surgery, Faculty of Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey

    S. Ergun

  6. Department of General Surgery, Istanbul Avcılar Murat Koluk State Hospital, Istanbul, Turkey

    S. Ergun

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Contributions

Gulsah KOC: Conception and design, genetic analysis, acquisition of data and interpretation, writing and final approval of the manuscript.

Ahu SOYOCAK: Conception and design, genetic analysis, interpretation of data.

Dilek DUZGUN ERGUN: Trace element and mineral measurement, calculation of data

Nural PASTACI OZSOBACI: Trace element and mineral measurement, calculation of data

Serap ANDAC OZTURK: Interpretation of data and writing

Sefa ERGUN: Collection of patient samples, acquisition of data.

All authors participated in the study design, reviewed and revised the manuscript, and approved the final manuscript.

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Correspondence to G. Koc.

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The research conforms to the provisions of the Declaration of Helsinki (as revised in Brazil 2013). All participants gave informed consent for the research. The study was approved by Istanbul Aydin University Clinical Trials Ethics Committee (B.30.2.AYD.0.00.00-050.06.04/576).

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Koc, G., Soyocak, A., Duzgun Ergun, D. et al. Association of TRPM5 Asn235Ser Polymorphism and Trace Elements/Minerals in Chronic Gastritis Patients: a Case-Control Study. Biol Trace Elem Res 200, 535–542 (2022). https://doi.org/10.1007/s12011-021-03002-8

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