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Zinc-Enriched Yeast May Improve Spermatogenesis by Regulating Steroid Production and Antioxidant Levels in Mice

Biological Trace Element Research volume 200, pages 3712–3722 (2022)Cite this article

Abstract

Zinc (Zn) is an essential nutrient for the human body. This nutrient is involved in numerous physiological functions and plays an important role in spermatogenesis. Zn-enriched yeast (ZnY) is considered a Zn supplement with high bioavailability and is widely used as a functional food. However, the effect of ZnY on male reproductive function remains unclear. This study aimed to investigate the beneficial effects of ZnY on the treatment of male spermatogenesis disorders. The spermatogenic dysfunctional mice were established by using cyclophosphamide (CP). CP was administered in saline at a dose of 50 mg/kg bw/day for 5 days by intraperitoneal injection (i.p.). Then, ZnY was orally supplemented at the dose levels of 2, 4, and 8 mg Zn/kg bw/day for 30 days. CP significantly decreased the sperm density and viability, testicular marker enzymes, serum testosterone, follicular stimulating hormone (FSH), and luteinizing hormone (LH). ZnY supplementation significantly improved these sperm parameters and hormone levels. Additionally, ZnY decreased the CP-induced lipid peroxidation and increased the glutathione levels. Moreover, ZnY increased the gene expression of anti-apoptotic proteins and steroid synthetase in mouse testes. The low-dose ZnY supplementation has a better effect on improving spermatogenesis, while the other two groups are less beneficial roles possibly due to excessive Zn intake. The present results suggest that appropriate ZnY can act as an accessory factor to improve steroid production and antioxidant levels in spermatogenic dysfunction mice.

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Data Availability

All data presented in the main manuscript.

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Acknowledgements

We are grateful to all members of Professor CH. Wang’s laboratory of the School of Health Sciences in Wuhan University, for their generous assistances.

Funding

This study was supported by grants from the Angel Nutritech Nutrition Fund (Grant NO. AF2019004).

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Authors and Affiliations

  1. Department of Preventive Medicine, School of Health Sciences, Wuhan University, Wuhan, 430071, Hubei Province, People’s Republic of China

    Zhaoyu Zhang, Cheng Peng, Ziqiong Wang, Haitao Ma, Duanya Liu, Lei Wang & Chunhong Wang

  2. Angel Nutritech Company Limited, Yichang, 443000, Hubei Province, People’s Republic of China

    Qian Cheng & Youjiao Liu

Authors
  1. Zhaoyu Zhang
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  2. Qian Cheng
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  3. Youjiao Liu
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  4. Cheng Peng
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  5. Ziqiong Wang
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  6. Haitao Ma
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  7. Duanya Liu
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  8. Lei Wang
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  9. Chunhong Wang
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Contributions

Chunhong Wang and Zhaoyu Zhang designed this study. Ziqiong Wang, Duanya Liu, Haitao Ma, and Lei Wang raised the animals. Peng Cheng and Zhaoyu Zhang carried out index detection and wrote the first draft. Chunhong Wang, Qian Cheng, and Youjiao Liu edited the final paper. All authors approved the final version.

Corresponding author

Correspondence to Chunhong Wang.

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This study was approved by the Institutional Animal Care and Use Committee of Animal Experiment Center of Wuhan University (IACUC) (Permit No. WP2020-08053).

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Zhang, Z., Cheng, Q., Liu, Y. et al. Zinc-Enriched Yeast May Improve Spermatogenesis by Regulating Steroid Production and Antioxidant Levels in Mice. Biol Trace Elem Res 200, 3712–3722 (2022). https://doi.org/10.1007/s12011-021-02970-1

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Keywords

  • Zinc-enriched yeast
  • Spermatogenesis
  • Male hormones
  • Antioxidants