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A Two-Generation Reproductive Toxicity Study of Lanthanum Nitrate in SD Rats

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Abstract

In order to evaluate the effects of lanthanum nitrate on the development of the parent, offspring, and the third generation of Sprague–Dawley (SD) rats, a two-generation reproductive toxicity experiment, was conducted. Two hundred and forty specific pathogen-free (SPF) healthy SD rats were randomly divided into the control group, low-, medium-, and high-dose group, with 30 male and 30 female rats in each group. The rats in each group were given 0 mg/kg, 10.0 mg/kg, 30.0 mg/kg, and 90.0 mg/kg lanthanum nitrate by gavage, respectively. There was no statistically significant difference between the weight gain and food intake of rats in each group. High-dose lanthanum nitrate had no effect on rat implantation and no embryo toxicity. The absolute and relative liver weights of F1a and F1b male rats in the high-dose group were significantly decreased. The absolute liver and spleen weight of F1b female rats in the high-dose group decreased significantly, but the relative weight did not change significantly. Histopathological examination results showed that there were no significant differences in the effects of different doses of lanthanum nitrate on the uterus, ovaries, oviduct, testes and epididymis, and liver of SD rats. Under the experimental conditions, 90.0 mg/kg lanthanum nitrate had an effect on the liver weight of the SD rats, but there was no liver toxicity. The no visible harmful effect level (NOAEL) of lanthanum nitrate on SD rats’ reproduction toxicity is 90 mg/kg.

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All the data in this study are available from the corresponding author on reasonable request.

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Funding

The study was funded by the National Key R&D Program of China (Grant No. 2017YFC1600203).

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Author notes

  1. Lang Yan, Fangyuan Gao and Wenjing Shi contributed equally to this work.

Authors and Affiliations

  1. Department of Health Toxicology, Faculty of Naval Medicine, The Second Military Medical University, Shanghai, 200433, China

    Lang Yan, Fangyuan Gao, Wenjing Shi, Jiqianzhu Zhang, Jingjing Mao, Yijun Tian, Xiaoyu Dai, Jikuai Chen, Jiangbo Zhu & Xiaofang Zhang

  2. School of Environmental Engineering, Shanghai University, Shanghai, 200444, China

    Bijiang Geng & Lijun Ren

Authors
  1. Lang Yan
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Contributions

Lang Yan, Fangyuan Gao, Wenjing Shi, and Xiaofang Zhang contributed to the experiment design, manuscript draft, and revision of the manuscript. Jingjing Mao contributed to the interpretation of pathological results. Bijiang Geng, Jiqianzhu Zhang, Yijun Tian, Lijun Ren, and Xiaoyu Dai contributed to the experiment implementation and data analysis. Jikuai Chen and Jiangbo Zhu analyzed the data. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Jikuai Chen, Jiangbo Zhu or Xiaofang Zhang.

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Ethics approval

The ethics governing the use and conduct of experiments on animals were strictly observed, and the experimental protocol was approved by the Committee on Ethics of Biomedicine, Second Military Medical University (Approval number: IACUC-2017256).

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The authors declare no competing interests.

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Yan, L., Gao, F., Shi, W. et al. A Two-Generation Reproductive Toxicity Study of Lanthanum Nitrate in SD Rats. Biol Trace Elem Res 200, 2268–2282 (2022). https://doi.org/10.1007/s12011-021-02841-9

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  • DOI: https://doi.org/10.1007/s12011-021-02841-9

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