Abstract
Chronic excessive fluoride exposure may lead to fluorosis, which causes health problems like a decrease in bone mechanical strength. It was speculated that chitosan may combine with fluorine to form in vivo organic fluorine, and may reduce the damage caused by fluorine. Hence, it is necessary to conduct a study to investigate the influence of chitosan on fluorosis mice. To investigate this problem, forty-four 4-week-old male Kunming mice were randomly divided into four groups, the control group, the fluoride group, the fluoride plus chitosan group, and the chitosan group. After 100 days of feeding, the femurs were collected to scan the Micro-CT image. The ultimate load of the femur in the fluoride group was significantly lower than control group. The trabecular separation was increased in the fluoride group compared with the fluoride plus chitosan group and the chitosan group. The level of trabecular thickness was increased in the fluoride plus chitosan group compared with the fluoride group. Our findings suggest that chitosan ingestion can improve the condition of cancellous bone and cortical bone affected by fluorine.
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The datasets analyzed during the current study are available from the corresponding author on reasonable request.
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This research was financially supported by the National Natural Science Foundation of China under grant no. 31972751.
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S.B.: designing and conducting the experiments, writing original draft preparation; A.H.: sample treatment, writing original draft preparation and editing; G.L.: data curation, data analysis, and image processing. Z.C.: sample treatment and data analysis. J.W. and J.W.: supervision and funding acquisition. The order of the co-first authors was assigned on the basis of their relative contributions to the study.
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Bian, S., Hu, A., Lu, G. et al. Study of Chitosan Ingestion Remitting the Bone Damage on Fluorosis Mice with Micro-CT. Biol Trace Elem Res 200, 2259–2267 (2022). https://doi.org/10.1007/s12011-021-02838-4
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DOI: https://doi.org/10.1007/s12011-021-02838-4