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Extra-Fortification of Zinc Upsets Vitellogenin Gene Expression and Antioxidant Status in Female of Clarias magur brooders

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Abstract

The present experiment was designed to evaluate the effect of graded level of zinc on Vitellogenin gene (Vtg) expression and antioxidant enzymes in threatened catfish, Clarias magur (C. magur). One hundred and eighty female C. magur with an average weight of 145 ± 5 g were allocated in twelve cemented tanks with dimension 4.5 × 2 × 1 m for a period of 60 days. Fish were distributed in four groups with three replicates following the completely randomised design. The first group treated as control (C) fed with basal diet contained normal zinc level, and remaining groups were fed with basal diets having 50, 200 and 300 mg/kg zinc acetate and treated as T1, T2 and T3 respectively. To evaluate the effect of dietary zinc supplementation on Vtg gene expression, three sampling were carried out, I sampling (April, before starting the experimental trail), II sampling (May, after 1 month of feeding trail) and III sampling (June before breeding season). In the present study, a dose-dependent relationship between Vtg gene expression and zinc inclusion in the diet of threatened catfish, C. magur, was reported. Vtg gene expression increased in all groups from I sampling to II sampling but the highest Vtg gene expression was found in T1 group and the lowest in T3 group at II sampling. Vtg gene expression among the treatments differs significantly (P < 0.05) in each sampling. Accumulation of zinc was measured by Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES) in C. magur and it was reported that the significantly higher (P < 0.05) zinc was accumulated in the liver and ovary of T3 group as compared to other groups. The antioxidant enzyme activities (superoxide dismutase, SOD, catalase and GST) were also measured in different tissues (liver, gill and ovary) to evaluate the effect of extra-supplementation of zinc on the antioxidant status. In T3 group, SOD, catalase and GST activities were significantly higher than those in other groups. In the current study, serum glucose level was also measured and it was found in increasing trend with inclusion of zinc in the diet of C. magur. In the present study, it can be concluded that the zinc exhibits beneficial effect only up to 50 mg/kg. Thus, it is concluded that supplementation of zinc at 200 mg/kg or more disrupts Vtg gene expression and antioxidant status.

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  1. Prem Prakash Srivastava

    Present address: Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur - 848 125, Bihar, India

Authors and Affiliations

  1. Division of Fish Nutrition, Biochemistry and Physiology, ICAR-Central Institute of Fisheries Education, Off Yari Road, Panch Marg, Versova, Mumbai-400 061, Maharashtra, India

    Gyandeep Gupta, Prem Prakash Srivastava, Munish Gangwar, Tincy Varghese, Subodh Gupta & Prasanta Jana

  2. Division of Aquaculture, ICAR-Central Institute of Fisheries Education, Off Yari Road, Panch Marg, Maharashtra, Versova, Mumbai-400 061, India

    Thongam I. Chanu

  3. ICAR-Central Institute of Fisheries Education Kakinada Centre, Near Old Burma Shell, Beach Road, Andhra Pradesh, Kakinada-533 007, India

    Muralidhar P. Ande

  4. ICAR-Central Institute of Fisheries Education, Off Yari Road, Panch Marg, Versova, Mumbai-400 061, Maharashtra, India

    Gopal Krishna

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Contributions

Gyandeep Gupta: conceptualisation and data curation; Prem Prakash Srivastava: conceptualisation, formal analyses, sources and software; Gyandeep Gupta, Prem Prakash Srivastava: roles in writing-original draft. Tincy Varghese: writing-review and editing and supervision; Thongam I. Chanu, Subodh Gupta, Muralidhar P. Ande, Gopal Krishna: review and editing; Prashana Jana: help in sampling, review and editing.

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Correspondence to Prem Prakash Srivastava.

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Gupta, G., Srivastava, P.P., Gangwar, M. et al. Extra-Fortification of Zinc Upsets Vitellogenin Gene Expression and Antioxidant Status in Female of Clarias magur brooders. Biol Trace Elem Res 200, 1861–1871 (2022). https://doi.org/10.1007/s12011-021-02793-0

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