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Anti-Hyperuricemic Effect of Iron Oxide Nanoparticles against Monosodium Urate Crystals Induced Gouty Arthritis in BALB/c Mice

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Abstract

Iron oxide nanoparticles (FeO-NPs) exhibit exceptional properties which can be utilized in various aspects of biological sciences. In this experiment we investigated the anti-gout effectiveness of FeO-NPs in mice. BALB/c mice were induced gouty arthritis by administering monosodium urate (MSU) crystals. These gout induced mice were treated with three different concentrations of FeO-NPs (5 ppm, 10 ppm and 20 ppm). Precipitation method was utilized for the synthesis of FeO-NPs, these synthesized NPs were of average 54 nm in size and were characterized using XRD, SEM and EDS. FeO-NPs is given orally three weeks by using FeO-NPs solution to substitute drinking water. Blood biochemical parameters including liver function tests (LFTs), renal function tests (RFTs), lipid profile and blood count have been tested. It has been found that uric acid, blood urea and creatinine have decreased significantly after three weeks of FeO-NP administration (P Value < 0.001) thus suppressing hyperuricemia and gouty arthritis. Additionally, the liver enzymes analysis showed a slight increase in AST, ALT and alkaline phosphatase levels (P Value < 0.001). Histopathological research revealed no significant abnormal changes in the liver, muscle and kidney muscles of the test groups. The findings showed that FeO-NPs can be used for the successful treatment of hyperuricemic condition and gouty arthritis in the coming future in place of commercially available medicines.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Shifa College of Medical Technology, Shifa Tameer-e-Millat University, Islamabad, Pakistan

    Mubin Mustafa Kiyani

  2. Rawal Institute of Health Sciences, Shaheed Zulfiqar Ali Bhutto Medical University (SZABMU), Islamabad, Pakistan

    Nurain Baig Moghul

  3. Faculty of Rehabilitation and Allied Health Sciences, Riphah International University, Islamabad, Pakistan

    Maisra Azhar Butt

  4. Department of Bioinformatics and Biotechnology, International Islamic University, Islamabad, Pakistan

    Hamza Rehman & Syed Ali Imran Bokhari

  5. Department of Rehabilitation Sciences, University of Lahore, Islamabad, Pakistan

    Ramsha Masood

  6. Faculty of Pharmaceutical & Allied Health Sciences, Shifa Tameer-e-Millat University, Islamabad, Pakistan

    Tausif Ahmed Rajput

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  1. Mubin Mustafa Kiyani
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  2. Nurain Baig Moghul
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  3. Maisra Azhar Butt
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  4. Hamza Rehman
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  5. Ramsha Masood
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  6. Tausif Ahmed Rajput
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  7. Syed Ali Imran Bokhari
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Correspondence to Mubin Mustafa Kiyani.

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Highlights

• Iron oxide nanoparticles (FeO-NPs) as therapeutic agent against hyperurecemia.

• Gouty arthritis induced by Monosodium Urate crystals.

• Discharge of uric acid decreased through urine due to deficiency of xanthine oxidase enzymes.

• FeO-NPs utilized for treatment of gouty arthritis.

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Kiyani, M.M., Moghul, N.B., Butt, M.A. et al. Anti-Hyperuricemic Effect of Iron Oxide Nanoparticles against Monosodium Urate Crystals Induced Gouty Arthritis in BALB/c Mice. Biol Trace Elem Res 200, 1659–1666 (2022). https://doi.org/10.1007/s12011-021-02769-0

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  • DOI: https://doi.org/10.1007/s12011-021-02769-0

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