Abstract
A total of 158 serum samples of newly diagnosed type 2 diabetes patients and control subjects were analyzed using Synchrotron Radiation X-ray Fluorescence (SRXRF) technique. The microprobe XRF beam line-16 of Indus-2 synchrotron radiation facility at Raja Ramanna Centre for Advanced Technology (RRCAT), Indore, India, was used to identify and quantify the elements K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Br, Rb, Sr, and Pb. A significant decrease in the mean concentrations of K, Ca, Ti, Cr, Mn, Ni, Zn, and As and an increase in the concentrations of V, Fe, Co, Cu, Se, and Pb were observed in the serum samples of the patient group when compared to the control group. It is hypothesized that the observed alterations in the elemental concentrations might have led to ineffective uptake of insulin and have interfered with glucose homeostasis by either directly or indirectly causing oxidative stress.
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The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
One of the authors, P. Sarita, acknowledges the financial support rendered by University Grants Commission -Department of Atomic Energy Consortium for Scientific Research, Indore Centre, India, to carry out this work in the form of a Collaborative Research Scheme (CSR-IC-BL-60/CRS-177/2016-17/841 dated 28th October 2016). The authors thank the authorities and staff of Indus-2 synchrotron radiation utilization facility at Raja Ramanna Centre for Advanced Technology (RRCAT), Indore, India, for the providing technical assistance. The authors appreciate the support of the medical personnel of Endocrinology Department, Andhra Medical College, Visakhapatnam, during sample collection.
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This research is supported by University Grants Commission – Department of Atomic Energy Consortium for Scientific Research, Indore Centre, India, in the form a sanctioned Collaborative Research Scheme (CRS) (CSR-IC-BL-60/CRS-177/2016–17/841 dated 28th October 2016).
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Rao, N.S., Raju, G.J.N., Tiwari, M.K. et al. Serum Elemental Analysis of Type 2 Diabetes Patients Using SRXRF. Biol Trace Elem Res 200, 1485–1494 (2022). https://doi.org/10.1007/s12011-021-02762-7
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DOI: https://doi.org/10.1007/s12011-021-02762-7