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Synthesis of Silver- and Strontium-Substituted Hydroxyapatite with Combined Osteogenic and Antibacterial Activities

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Abstract

Infection in bone transplantation process is attracting considerable attention. The current study synthesizes silver/strontium co-substituted hydroxyapatite (Ag/Sr-HA) nanoparticles with combined osteogenic and antibacterial activities. Different concentrations of silver-substituted hydroxyapatite (Ag-HA) nanoparticles were synthesized by hydrothermal method, and then their physicochemical properties were characterized by X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), and energy-dispersive X-ray spectroscopy (EDS). Then, Sr was added as secondary element into Ag-HA to improve the biocompatibility of substrate. The antibacterial experiments indicated that Ag-HA had excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The effects of prepared samples on cell proliferation and differentiation were evaluated using MC3T3-E1 cells in vitro. The results showed that Sr substitution enhanced cell proliferation and differentiation, upregulated expression of osteogenic genes, and induced mineralization of cells. The substitution of Sr in Ag/Sr-HA nanoparticles can effectively alleviate the negative effects of Ag and enhance the biological activity of HA. Thus, the synthesized Ag/Sr-HA nanoparticles will serve as a potential candidate for application of biomedical implants with excellent osteogenic and antibacterial ability.

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Funding

This work was supported by the National Natural Science Foundation of China (21471044), the Natural Science Foundation of Hebei Province (B2020201020), the Science and Technology Projects of Hebei Education Department (ZD2020150), the Third Batch of Top Youth Talent Support Program of Hebei Province, the Priority Strategy Project of Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education (ts2019006), Medical Science Foundation of Hebei University (2020A03), and the Post-graduate,s Innovation Fund Project of Hebei University (hbu2020ss012).

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Authors and Affiliations

  1. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Chemical Biology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China

    Yunfei Li, Wenying Wang, Jing Han, Zirui Li, Qiuxiang Wang, Xue Lin, Kun Ge & Guoqiang Zhou

  2. Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-autoimmune Diseases of Hebei Province, College of Basic Medical Sciences, Hebei University, Baoding, 071000, China

    Guoqiang Zhou

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  1. Yunfei Li
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Li, Y., Wang, W., Han, J. et al. Synthesis of Silver- and Strontium-Substituted Hydroxyapatite with Combined Osteogenic and Antibacterial Activities. Biol Trace Elem Res 200, 931–942 (2022). https://doi.org/10.1007/s12011-021-02697-z

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