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Effect of Fluoride in Drinking Water on Fecal Microbial Community in Rats

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Abstract

Intestinal nutrition has a close association with the onset and development of fluorosis. Intestinal microbes play a major role in intestinal nutrition. However, the effect of fluoride on intestinal microbes is still not fully understood. This study aimed to evaluate the dose-response of fluoride on fecal microbes as well as the link between fluorosis and fecal microbes. The results showed that fluoride did not significantly alter the diversity of fecal microbiota, but richness estimators (ACE and Chao) increased first, and then decreased with the increase of water fluoride. At the genus level, 150 mg/L fluoride significantly reduced the abundances of Roseburia and Clostridium sensu stricto, and 100 mg/L and 150 mg/L fluoride obviously increased the abundances of Unclassified Ruminococcaceaes and Unclassified Bdellovibrionales, respectively. The correlation analysis showed fluoride exposure had a negative association with Roseburia and Turicibacter and was positively associated with Pelagibacterium, Unclassified Ruminococcaceae, and Unclassified Bdellovibrionales. Dental fluorosis was negatively associated with Clostridium sensu stricto, Roseburia, Turicibacter, and Paenalcaligenes and had a positive association with Pelagibacterium, Unclassified Ruminococcaceae, and Unclassified Bdellovibrionales. In conclusion, this study firstly reports fluoride in drinking water has a remarkable biphasic effect on fecal microbiota in rats, and some bacteria are significantly associated with fluoride exposure and dental fluorosis. These results indicate the gut microbiota may play an important role in fluorosis, and some bacteria are likely to be developed as biomarkers for fluorosis.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was supported by the National Natural Science Foundation of China (81773468 and 81302389), the Wu Liande Science Foundation of Harbin Medical University (Grant No. WLD-QN1703), and the Postdoctoral Scientific Research Developmental Fund of Heilongjiang Province (LBH-Q17092).

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Authors and Affiliations

  1. Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & National Health Commission (23618504), Institute for Kaschin-Beck Disease Control, Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, 150081, Heilongjiang Province, China

    Nan Zhong, Yongzheng Ma, Xinyue Meng, Alphonse Sowanou & Junrui Pei

  2. Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & National Health Commission (23618504), Institute for Fluorosis Disease Control, Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, 150081, Heilongjiang Province, China

    Liaowei Wu, Wei Huang & Yanhui Gao

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  1. Nan Zhong
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Contributions

Conceived and designed the experiments: Junrui Pei

Raised the animals: Liaowei Wu and Wei Huang

Performed the experiments: Nan Zhong, Yongzheng Ma, Xinyue Meng, and Junrui Pei

Contributed reagents/materials/analysis tools: Junrui Pei and Yanhui Gao

Wrote the paper: Junrui Pei, Nan Zhong, Yongzheng Ma, Xinyue Meng, and Alphonse Sowanou

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Correspondence to Junrui Pei.

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Zhong, N., Ma, Y., Meng, X. et al. Effect of Fluoride in Drinking Water on Fecal Microbial Community in Rats. Biol Trace Elem Res 200, 238–246 (2022). https://doi.org/10.1007/s12011-021-02617-1

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