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The Protective Effect of Selenium on T-2-Induced Nephrotoxicity Is Related to the Inhibition of ROS-Mediated Apoptosis in Mice Kidney

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Abstract

T-2 toxin is produced by the Fusarium genus. Ingestion of food or feed contaminated by T-2 toxin will cause damage to kidney. Selenium (Se), an essential trace element, showed the significant protective effects against kidney and renal cell damage induced by toxic substances. To explore the protective effects and mechanisms of Se against T-2-induced renal lesions, forty-eight male Kunming mice were exposed to T-2 toxin (1.0 mg/kg) and/or Se (0.2 mg/kg) for 28 days. In this study, we found that Se alleviated T-2-induced nephrotoxicity, presenting as increasing the body weight and kidney coefficient, relieving the renal structure injury, decreasing the contents of renal function-related biomarkers, decreasing the levels of reactive oxygen species (ROS), and increasing the mitochondrial membrane potential in T-2 toxin-treated mice. In addition, inhibition of renal cell apoptosis by Se was associated with blocking the mitochondrial pathway in T-2 toxin-treated mice, presenting as decreasing the protein expression of cytochrome-c, activities of caspase-3/9, as well as regulating the protein and mRNA expressions of Bax and Bcl-2. These results documented that the alleviating effect of Se on T-2-induced nephrotoxicity is related to the inhibition of ROS-mediated renal apoptosis.

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Abbreviations

Se:

Selenium

BW:

Body weight

ROS:

Reactive oxygen species

MMP:

Mitochondrial membrane potential

Cyt-c:

Cytochrome-c

TEM:

Transmission electron microscope

GSG-Px:

Glutathione peroxidase

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Funding

This study was supported by China Postdoctoral Science Foundation (2020M682296), the Scientific and Technological Project of Henan Province-China (212102110362), and the Heilongjiang provincial natural science foundation of China/C2018020.

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Authors and Affiliations

  1. Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, NO. 600, Changjiang Road, Xiangfang District, Harbin, 150030, Heilongjiang, China

    Xuliang Zhang, Qi Wang, Jian Zhang, Miao Song, Bing Shao, Yanfei Han & Yanfei Li

  2. College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, Henan, China

    Xu Yang

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  1. Xuliang Zhang
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  2. Qi Wang
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  3. Jian Zhang
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Correspondence to Yanfei Li.

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Zhang, X., Wang, Q., Zhang, J. et al. The Protective Effect of Selenium on T-2-Induced Nephrotoxicity Is Related to the Inhibition of ROS-Mediated Apoptosis in Mice Kidney. Biol Trace Elem Res 200, 206–216 (2022). https://doi.org/10.1007/s12011-021-02614-4

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