Abstract
An in vitro system employing collagen isolated from the sheep tendons to induce mineralization and demineralization reactions was used not only to study the effect of various concentrations of fluoride on the collagen-induced mineralization and demineralization reactions but also to compare their action with the inhibitors of mineralization and/or demineralization. Studies demonstrated that under physiological conditions, at lower concentrations (5 × 10−6 to 5 × 10−5 M) fluoride inhibited while at higher concentrations (> 10−4 M), it stimulated the collagen-induced in vitro mineralization. At higher concentrations, fluoride was also found to inhibit the demineralization of the collagen bound preformed mineral phase. At low concentrations, fluoride acted like Mg2+ to inhibit mineralization while at higher concentration, it acted like crystal poisons (e.g., pyrophosphate phosphonates, citrate) to inhibit demineralization. However, unlike magnesium and pyrophosphate, fluoride at its higher concentrations was found to stimulate rather than inhibit the process of mineralization.
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References
Glimcher MJ (1959) Molecular biology of mineralized tissues with particular reference to bone. Rev Mod Phys 31:359–393. https://doi.org/10.1103/RevModPhys.31.359
Cheng P-T (1985) Octacalcium phosphate formationin vitro: implications for bone formation. Calcif Tissue Int 37:91–94. https://doi.org/10.1007/BF02557685
Cadet ER, Gafni RI, Mccarthy EF, Mccray DR, Bacher JD, Barnes KM, Baron J Mechanisms responsible for longitudinal growth of the cortex. J Bone Jt Surg Am 85(2003):1739–1748. https://doi.org/10.2106/00004623-200309000-00013
Singh SP, Singh R, Jethi RK (1982) Kinetics of in vitro aorta mineralization. Indian J Exp Biol 20:691–695 http://www.ncbi.nlm.nih.gov/pubmed/7160869 (accessed April 5, 2020)
Margolis HC, Kwak S-Y, Yamazaki H (2014) Role of mineralization inhibitors in the regulation of hard tissue biomineralization: relevance to initial enamel formation and maturation. Front Physiol 5:339. https://doi.org/10.3389/fphys.2014.00339
Talwar HS, Jethi RK (1978) Role of collagen in ion uptake & exchange reactions. Indian J Exp Biol 16:187–190 http://www.ncbi.nlm.nih.gov/pubmed/680810 (accessed April 5, 2020)
Jethi RK, Inlow CW, Wadkins CL (1970) Studies of the mechanism of biological calcification. Calcif Tissue Res 6:81–92. https://doi.org/10.1007/BF02196187
Jethi RK, Wadkins CL (1971) Studies of the mechanism of biological calcification. Calcif Tissue Res 7:277–289. https://doi.org/10.1007/BF02062617
Jethi RK, Chander L, Singh J (1977) Kinetic evidence for a step-wise process in collagen-induced in vitro calcification. Indian J Exp Biol 15:35–39 http://www.ncbi.nlm.nih.gov/pubmed/908590 (accessed April 5, 2020)
Blumenthal NC, Posner AS (1984) In vitro model of aluminum-induced osteomalacia: Inhibition of hydroxyapatite formation and growth. Calcif Tissue Int 36:439–441. https://doi.org/10.1007/BF02405357
Blumenthal NC (1989) Mechanisms of inhibition of calcification. Clin Orthop Relat Res NA:279–289. https://doi.org/10.1097/00003086-198910000-00038
Tandon CD, Forouzandeh M, Aggarwal S, Jethi RK (1997) Inhibitors of in vitro mineralization from flexor tendons of rabbits and their role in biological mineralization. Mol Cell Biochem 171:29–35. https://doi.org/10.1023/a:1006894400172
Aggarwal S, Tandon CD, Forouzandeh M, Singla SK, Kiran R, Jethi RK (2000) Role of biomolecules from human renal stone matrix on COM crystal growth. Mol Cell Biochem 210:109–119. https://doi.org/10.1023/a:1007109120558
Gupta LC, Singla SK, Tandon C, Jethi RK (2004) Mg2+: a potent inhibitor of collagen-induced in vitro mineralization. Magnes Res 17:67–71 http://www.ncbi.nlm.nih.gov/pubmed/15319136 (accessed April 5, 2020)
Moghadam MF, Tandon C, Aggarwal S, Singla SK, Singh SK, Sharma SK, Varshney GC, Jethi RK (2003) Concentration of a potent calcium oxalate monohydrate crystal growth inhibitor in the urine of normal persons and kidney stone patients by ELISA-based assay system employing monoclonal antibodies. J Cell Biochem 90:1261–1275. https://doi.org/10.1002/jcb.10671
Taves DR (1966) Normal human serum fluoride concentrations. Nature. 211:192–193. https://doi.org/10.1038/211192b0
Aoba T (1997) The effect of fluoride on apatite structure and growth. Crit Rev Oral Biol Med 8:136–153. https://doi.org/10.1177/10454411970080020301
K.L. Kirk, Biochemistry of inorganic fluoride, in: Biochem. Elem. Halogens Inorg. Halides, Springer US, Boston, 1991: pp. 19–68. https://doi.org/10.1007/978-1-4684-5817-6_2\
Medjedovic E, Medjedovic S, Deljo D, Sukalo A (2015) Impact of fluoride on dental health quality. Mater Socio Medica 27:395–398. https://doi.org/10.5455/msm.2015.27.395-398
Pajor K, Pajchel L, Kolmas J (2019) Hydroxyapatite and fluorapatite in conservative dentistry and oral implantology—a review. Materials (Basel) 12:2683. https://doi.org/10.3390/ma12172683
Everett ET (2011) Fluoride’s effects on the formation of teeth and bones, and the influence of genetics. J Dent Res 90:552–560. https://doi.org/10.1177/0022034510384626
Vieira A, Hancock R, Dumitriu M, Schwartz M, Limeback H, Grynpas M (2005) How does fluoride affect dentin microhardness and mineralization? J Dent Res 84:951–957. https://doi.org/10.1177/154405910508401015
Baginski ES, Marie SS, Clark WL, Zak B (1973) Direct microdetermination of serum calcium. Clin Chim Acta 46:46–54. https://doi.org/10.1016/0009-8981(73)90101-0
Amador E, Urban J (1972) Simplified serum phosphorus analyses by continuous-flow ultraviolet spectrophotometry. Clin Chem 18:601–604 http://www.ncbi.nlm.nih.gov/pubmed/5037903 (accessed April 5, 2020)
McGaughey C (1983) Binding of polyphosphates and phosphonates to hydroxyapatite, subsequent hydrolysis, phosphate exchange and effects on demineralization, mineralization and microcrystal aggregation. Caries Res 17:229–241. https://doi.org/10.1159/000260671
Godel J, Bay H, Columbia B (2002) The use of fluoride in infants and children. Paediatr Child Health 7:569–572. https://doi.org/10.1093/pch/7.8.569
Walsh T, Worthington HV, Glenny AM, Marinho VCC, Jeroncic A (2019) Fluoride toothpastes of different concentrations for preventing dental caries. Cochrane Database Syst Rev 2019. https://doi.org/10.1002/14651858.CD007868.pub3
FLEMING HS, GREENFIELD VS (1954) Changes in the teeth and jaws of neonatal webster mice after administration of NaF and CaF2 to the female parent during gestation. J Dent Res 33:780–788. https://doi.org/10.1177/00220345540330060601
Angmar-Månsson B, Ericsson Y, Ekberg O (1976) Plasma fluoride and enamel fluorosis. Calcif Tissue Res 22:77–84. https://doi.org/10.1007/BF02010348
Rafique T, Ahmed I, Soomro F, Khan K (2015) Masood Hameed Shirin, Fluoride levels in urine, blood plasma and serum of people living in an endemic fluorosis area in the Thar Desert, Pakistan. J Chem Soc Pak 37:1223–1230
Acknowledgments
The authors are grateful to Mr. K. K. Maheshwari for standardizing the methodology and initiating the work as a research scholar before leaving for higher studies. The authors are indebted to Departments of Biochemistry at Panjab University Chandigarh in Punjab and Himalayan Institute of Medical Sciences at Dehradun in Uttrakhand, for providing facilities and necessary funds for the studies.
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Kakkar, M., Kapoor, V., Singla, S.K. et al. Fluoride and Biological Calcification I: Effect of Fluoride on Collagen-Induced In Vitro Mineralization and Demineralization Reactions. Biol Trace Elem Res 199, 2208–2214 (2021). https://doi.org/10.1007/s12011-020-02340-3
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DOI: https://doi.org/10.1007/s12011-020-02340-3