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Effects of Fluoride on Oxidative Stress Markers of Lipid, Gene, and Protein in Rats

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Abstract

Endemic fluorosis is a systemic chronic disease caused by excessive intake of fluoride. It is widely accepted that oxidative stress is closely related to fluorosis; however, molecular mechanism of oxidative stress in fluorosis remains unclear. This study investigated the effects of fluoride (F) on oxidative stress markers of lipid, gene, and protein in rats for revealing molecular mechanism of oxidative stress in fluorosis. The results showed concentration and exposure time of fluoride both had a significant effect on MDA and 8-OHdG. Fluoride concentration significantly impacted AGEs level, but exposure time did not. AOPP was not statistically different among the groups. AGEs decreased with the increase of fluoride in the rats with 3 months of fluoride treatment. The correlation analysis showed the degree of dental fluorosis was significantly negatively correlated with 8-OHdG at 1 month and 3 months, and negatively correlated with AGEs at 3 months. In the rats with 100 mg/L of fluoride treatment, MDA was significant positively correlated with 8-OHdG, and negatively correlated with AGEs. 8-OHdG was significantly negatively correlated with AGEs in the control group and 100 mg/L fluoride group. Taken together, fluoride had different effects on oxidative stress markers of lipid, gene, and protein. Excessive fluoride could increase MDA content, and decrease 8-OHdG and AGEs. These findings suggest that oxidative stress involved in molecular pathogenesis of fluorosis is complicated, and needs to furtherly study in the future.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was supported by National Natural Science Foundation of China (81773468 and 811302389), the Wu Liande Science Foundation of Harbin Medical University (Grant No. WLD-QN1703), and Postdoctoral Scientific Research Developmental Fund of Heilongjiang Province (LBH-Q17092).

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Conceived and designed the experiments: Junrui Pei. Performed the experiments: Nan Zhong, Yingjie Yao, Yongzheng Ma, Xinyue Meng, and Junrui Pei. Contributed reagents/materials/analysis tools: Junrui Pei. Wrote the paper: Junrui Pei, Nan Zhong, Yingjie Yao, and Alphonse Sowanou. Nan Zhong and Yingjie Yao contributed equally to this work.

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Correspondence to Junrui Pei.

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Zhong, N., Yao, Y., Ma, Y. et al. Effects of Fluoride on Oxidative Stress Markers of Lipid, Gene, and Protein in Rats. Biol Trace Elem Res 199, 2238–2246 (2021). https://doi.org/10.1007/s12011-020-02336-z

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