The green approach of nanoparticle synthesis has gained more attention by researchers because of its nontoxic, eco-friendly, biocompatible, and sustainable nature. The present research investigated the anticancer effectiveness of silver nanoparticles synthesized from marine algae Chaetomorpha linum (C. linum) against colon cancer cell HCT-116 in vitro. Biosynthesized silver nanoparticles (C-AgNPs) are characterized using UV-spectrophotometry, dynamic light scattering (DLS), X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). We demonstrated the dose-dependent cytotoxic effect of C-AgNPs in human colorectal carcinoma cells (HCT-116) using MTT assay. The apoptosis induction in HCT-116 cells caused by C-AgNPs has studied fluorescence microscope by staining with fluorogenic agents 4′,6-diamidino-2-phenylindole (DAPI), rhodamine 123, and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA). By using a flow cytometric test, the apoptotic action of C-AgNPs was performed. The immunoblotting study of caspases, as well as pro-apoptotic and anti-apoptotic protein expression, was studied using the PCR technique to understand the underlying molecular mechanism of C-AgNPs on cancer cells. The apoptotic studies showed an increase in the expression of apoptotic caspase 3, caspase 9, BH3-interacting domain death agonist (Bid), and Bax, along with a decrease in the anti-apoptotic protein like Bcl-2 and Bcl-xl, thereby veritably confirmed by immunoblotting and qPCR technique. The biosynthesized C-AgNPs was an efficient anticancer agent that can induce apoptosis in the HCT-116 colon cells.
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Guo Y-X, Zhang Z-Z, Zhao G, Zhao E-H (2019) Prognostic and pathological impact of tumor budding in gastric cancer: a systematic review and meta-analysis. World J Gastrointest Oncol 11(10):898–908
Qiao W, Wang B, Wang Y, Yang L, Zhang Y, Shao P (2010) Cancer therapy based on nanomaterials and nanocarrier systems. J Nanomater 2010:7
Somu P, Paul S (2019) Supramolecular nanoassembly of lysozyme and α-lactalbumin (apo α-LA) exhibits selective cytotoxicity and enhanced bioavailability of curcumin to cancer cells. Colloids Surf B: Biointerfaces 178:297–306
Salem SS, Fouda A (2020) Green synthesis of metallic nanoparticles and their prosective biotechnological applications: an overview. Biol Trace Elem Res. https://doi.org/10.1007/s12011-020-02138-3
Somu P, Paul S (2019) A biomolecule-assisted one-pot synthesis of zinc oxide nanoparticles and its bioconjugate with curcumin for potential multifaceted therapeutic applications. New J Chem 43(30):11934–11948
Swanner J, Fahrenholtz CD, Tenvooren I, Bernish BW, Sears JJ, Hooker A, Furdui CM, Alli E, Li W, Donati GL (2019) Silver nanoparticles selectively treat triple-negative breast cancer cells without affecting non-malignant breast epithelial cells in vitro and in vivo. FASEB Bioadv 1(10):639–660
Fouda A, Hassan SE-D, Abdo AM, El-Gamal MS (2019) Antimicrobial, antioxidant and larvicidal activities of spherical silver nanoparticles synthesized by endophytic Streptomyces Spp. Biol Trace Elem Res, 195: 707–724. https://doi.org/10.1007/s12011-019-01883-4
Kim JS, Kuk E, Yu KN, Kim J-H, Park SJ, Lee HJ, Kim SH, Park YK, Park YH, Hwang C-Y (2007) Antimicrobial effects of silver nanoparticles. Nanomedicine 3(1):95–101
Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27(1):76–83
Hassanen EI, Ragab E (2020) In vivo and in vitro assessments of the antibacterial potential of chitosan-silver nanocomposite against methicillin-resistant Staphylococcus aureus–induced infection in rats. Biol Trace Elem Res. https://doi.org/10.1007/s12011-020-02143-6
Sankar R, Karthik A, Prabu A, Karthik S, Shivashangari KS, Ravikumar V (2013) Origanum vulgare mediated biosynthesis of silver nanoparticles for its antibacterial and anticancer activity. Colloids Surf B: Biointerfaces 108:80–84
Gomathi A, Rajarathinam SX, Sadiq AM, Rajeshkumar S (2020) Anticancer activity of silver nanoparticles synthesized using aqueous fruit shell extract of Tamarindus indica on MCF-7 human breast cancer cell line. J Drug Deliv Sci Technol 55:101376
Kalantari K, Mostafavi E, Afifi AM, Izadiyan Z, Jahangirian H, Rafiee-Moghaddam R, Webster TJ (2020) Wound dressings functionalized with silver nanoparticles: promises and pitfalls. Nanoscale 12(4):2268–2291
Hassanen EI, Khalaf AA, Tohamy AF, Mohammed ER, Farroh KY (2019) Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats. Int J Nanomedicine 14:4723–4739
Xiong J, Wu X-d, Xue Q-j (2013) Biomolecule-assisted synthesis of highly stable dispersions of water-soluble copper nanoparticles. J Colloid Interface Sci 390(1):41–46
Somu P, Paul S (2019) Protein assisted one pot controlled synthesis of monodispersed and multifunctional colloidal silver-gold alloy nanoparticles. J Mol Liq 291:111303
Hassan SE-D, Fouda A, Radwan AA, Salem SS, Barghoth MG, Awad MA, Abdo AM, El-Gamal MS (2019) Endophytic actinomycetes Streptomyces spp mediated biosynthesis of copper oxide nanoparticles as a promising tool for biotechnological applications. J Biol Inorg Chem 24(3):377–393
Fouda A, Abdel-Maksoud G, Abdel-Rahman MA, Eid AM, Barghoth MG, El-Sadany MA-H (2019) Monitoring the effect of biosynthesized nanoparticles against biodeterioration of cellulose-based materials by Aspergillus niger. Cellulose 26(11):6583–6597
Fouda A, Abdel-Maksoud G, Abdel-Rahman MA, Salem SS, Hassan SE-D, El-Sadany MA-H (2019) Eco-friendly approach utilizing green synthesized nanoparticles for paper conservation against microbes involved in biodeterioration of archaeological manuscript. Int Biodeterior Biodegradation 142:160–169
Alsharif SM, Salem SS, Abdel-Rahman MA, Fouda A, Eid AM, Hassan SE-D, Awad MA, Mohamed AA (2020) Multifunctional properties of spherical silver nanoparticles fabricated by different microbial taxa. Heliyon 6(5):e03943
Minhajdeen A, Pavithra V, Brindha S, Saranya R, Varun S, Senthilkumar P, Sudha S (2014) Antioxidant and antifungal activities of methanolic extract of Chaetomorpha linum from Indian southeast coast. Int J Curr Res Rev 6(7):11
Stabili L, Acquaviva MI, Angilè F, Cavallo RA, Cecere E, Del Coco L, Fanizzi FP, Gerardi C, Narracci M, Petrocelli A (2019) Screening of chaetomorpha linum lipidic extract as a new potential source of bioactive compounds. Mar Drugs 17(6):313
Kannan RRR, Arumugam R, Ramya D, Manivannan K, Anantharaman P (2013) Green synthesis of silver nanoparticles using marine macroalga Chaetomorpha linum. Appl Nanosci 3(3):229–233
Aboelfetoh EF, El-Shenody RA, Ghobara MM (2017) Eco-friendly synthesis of silver nanoparticles using green algae (Caulerpa serrulata): reaction optimization, catalytic and antibacterial activities. Environ Monit Assess 189(7):349
Mahanta S, Prathap S, Ban DK, Paul S (2017) Protein functionalization of ZnO nanostructure exhibits selective and enhanced toxicity to breast cancer cells through oxidative stress-based cell death mechanism. J Photochem Photobiol B Biol 173:376–388
Elumalai P, Gunadharini D, Senthilkumar K, Banudevi S, Arunkumar R, Benson C, Sharmila G, Arunakaran J (2012) Induction of apoptosis in human breast cancer cells by nimbolide through extrinsic and intrinsic pathway. Toxicol Lett 215(2):131–142
Chomczynski P, Sacchi N (2006) The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on. Nat Protoc 1(2):581–585
Liu R, Pei Q, Shou T, Zhang W, Hu J, Li W (2019) Apoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells. Int J Nanomedicine 14:4091–4103
Venkatesan J, Kim S-K, Shim MS (2016) Antimicrobial, antioxidant, and anticancer activities of biosynthesized silver nanoparticles using marine algae Ecklonia cava. Nanomaterials 6(12):235
Soliman AM, Abdel-Latif W, Shehata IH, Fouda A, Abdo AM, Ahmed YM (2020) Green approach to overcome the resistance pattern of Candida spp. using biosynthesized silver nanoparticles fabricated by Penicillium chrysogenum F9. Biol Trace Elem Res. https://doi.org/10.1007/s12011-020-02188-7
Fouda A, Saad E, Salem SS, Shaheen TI (2018) In-vitro cytotoxicity, antibacterial, and UV protection properties of the biosynthesized zinc oxide nanoparticles for medical textile applications. Microb Pathog 125:252–261
Salem SS, Fouda MM, Fouda A, Awad MA, Al-Olayan EM, Allam AA, Shaheen TI (2020) Antibacterial, cytotoxicity and larvicidal activity of green synthesized selenium nanoparticles using Penicillium corylophilum. J Clust Sci. https://doi.org/10.1007/s10876-020-01794-8
Pounraj S, Somu P, Paul S (2018) Chitosan and graphene oxide hybrid nanocomposite film doped with silver nanoparticles efficiently prevents biofouling. Appl Surf Sci 452:487–497
Akbari B, Tavandashti MP, Zandrahimi M (2011) Particle size characterization of nanoparticles–a practicalapproach. Iran J Mater Sci Eng 8(2):48–56
Augustine R, Augustine A, Kalarikkal N, Thomas S (2016) Fabrication and characterization of biosilver nanoparticles loaded calcium pectinate nano-micro dual-porous antibacterial wound dressings. Prog Biomater 5(3–4):223–235
Bagur H, Poojari CC, Melappa G, Rangappa R, Chandrasekhar N, Somu P (2019) Biogenically synthesized silver nanoparticles using endophyte fungal extract of Ocimum tenuiflorum and evaluation of biomedical properties. J Clust Sci. https://doi.org/10.1007/s10876-019-01731-4
Gurunathan S, Qasim M, Park C, Yoo H, Kim J-H, Hong K (2018) Cytotoxic potential and molecular pathway analysis of silver nanoparticles in human colon cancer cells HCT116. Int J Mol Sci 19(8):2269
Somu P, Paul S (2019) HSP90 and its inhibitors for cancer therapy: use of nano-delivery system to improve its clinical application. In: Heat shock protein 90 in human diseases and disorders. Springer, pp 159–182
Shi J, Kantoff PW, Wooster R, Farokhzad OC (2017) Cancer nanomedicine: progress, challenges and opportunities. Nat Rev Cancer 17(1):20–37
Burduşel A, Gherasim O, Grumezescu A, Mogoant ÃL, Ficai A, Andronescu E (2018) Biomedical applications of silver nanoparticles: an up-to-date overview. Nanomaterials 8(9):681.
Singh AP, Biswas A, Shukla A, Maiti P (2019) Targeted therapy in chronic diseases using nanomaterial-based drug delivery vehicles. Signal Transduct Target Ther 4(1):1–21
Somu P, Kannan U, Paul S (2019) Biomolecule functionalized magnetite nanoparticles efficiently adsorb and remove heavy metals from contaminated water. J Chem Technol Biotechnol 94(6):2009–2022
Priyadharshini RI, Prasannaraj G, Geetha N, Venkatachalam P (2014) Microwave-mediated extracellular synthesis of metallic silver and zinc oxide nanoparticles using macro-algae (Gracilaria edulis) extracts and its anticancer activity against human PC3 cell lines. Appl Biochem Biotechnol 174(8):2777–2790
The authors would like to extend their sincere appreciation to the Director of NRME Research Laboratory, Newredmars Education Pvt. Ltd., for allowing for carrying out the research work. The authors are also thankful to Dr. Pralay Ranjan Behera, Scientist, Marine Biodiversity Division, Regional Centre of ICAR-Central Marine Fisheries Research Institute, Visakhapatnam, Andhra Pradesh, for the identification of algal species Chaetomorpha linum for analysis.
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Acharya, D., Satapathy, S., Somu, P. et al. Apoptotic Effect and Anticancer Activity of Biosynthesized Silver Nanoparticles from Marine Algae Chaetomorpha linum Extract Against Human Colon Cancer Cell HCT-116. Biol Trace Elem Res (2020). https://doi.org/10.1007/s12011-020-02304-7
- Chaetomorpha linum
- Silver nanoparticles
- Colon cancer