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Relationship Between Zinc Status and DNA Oxidative Damage in Patients with Type 2 Diabetes Mellitus

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Abstract

Although zinc deficiency increases the risk of oxidative DNA damage, data regarding the association between zinc and oxidative DNA damage in diabetes are controversial. In this article, we focus on serum zinc levels and its relation to an established biomarker of oxidative DNA damage (8-hydroxy-2-deoxyguanosine) in patients with type 2 diabetes, and to ascertain the beneficial effects of zinc supplementation on the level of oxidative DNA damage. The study consisted of 2 interrelated parts: The first part was a cross-sectional study conducted on patients with type 2 diabetes (n = 297) and healthy individuals (n = 188). The second part was an interventional study that enrolled 38 diabetic patients with low zinc status and high DNA damage. The demographic parameters including age, gender, and body mass index were recorded, and DNA damage marker through 8-hydroxy-2-deoxyguanosine levels, and zinc status of serum zinc, was measured. Significantly higher 8-hydroxy-2-deoxyguanosine levels (P < 0.00) together with lower zinc levels (P < 0.001) were found in the diabetics compared to healthy controls. Patients with low zinc status had higher levels of 8-hydroxy-2-deoxyguanosine compared to patients with normal zinc status. In diabetic patients, a negative correlation of 8-hydroxy-2-deoxyguanosine was observed with zinc (P = 0.070). Zinc supplementation showed a significant decrease in 8-hydroxy-2-deoxyguanosine by (26.0%) and increased in serum zinc by (42.0%). Elevated 8-hydroxy-2-deoxyguanosine levels in conjunction with low zinc status may indicate a high degree of oxidative DNA damage in diabetic patients. The results confirm that zinc supplementation in this group may help correct abnormal levels of 8-hydroxy-2-deoxyguanosine.

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References

  1. Leone N, Courbon D, Ducimetiere P, Zureik M (2006) Zinc, copper, and magnesium and risks for all-cause, cancer, and cardiovascular mortality. Epidemiology 17(3):308–314

    Article  Google Scholar 

  2. Simone S, Gorin Y, Velagapudi C, Abboud HE, Habib SL (2008) Mechanism of oxidative DNA damage in diabetes. Tuberin inactivation and downregulation of DNA repair enzyme 8-Oxo-7,8-dihydro-2′-Deoxyguanosine-DNA Glycosylase. Diabetes 57(10):2626–2636. https://doi.org/10.2337/db07-1579

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Lima VB, SampaioFde A, Bezerra DL, MoitaNeto JM, Marreiro DN (2011) Parameters of glycemic control and their relationship with zinc concentrations in blood and with superoxide dismutase enzyme activity in type 2 diabetes patients. Arq Bras EndocrinolMetabol 55(9):701–707

    Article  Google Scholar 

  4. Dandone P, Thusu K, Cook S, Snyder B, Makowski J, Armstrong D et al (1996) Oxidative damage to DNA in diabetes mellitus. Lancet 347:444–445

    Article  Google Scholar 

  5. Fenech M, Ferguson L (2001) Vitamins/minerals and genomic stability in humans. Mutat Res 475:1–6

    Article  CAS  Google Scholar 

  6. Song Y, Leonard SW, Traber MG, Ho E (2009) Zinc deficiency affects DNA damage, oxidative stress, antioxidant defenses, and DNA repair in rats. J Nutr 139(9):1626–1631

    Article  CAS  Google Scholar 

  7. Mahmoud HM, Al-Tmimi DJ (2012) Current zinc status among the healthy population in Duhok city, Iraq. Duhok Med J 6:15–20

    Google Scholar 

  8. Ruz M, Carrasco F (2013) Zinc as a potential coadjuvant in therapy for type 2 diabetes. Food Nutr Bull 34:215–221. https://doi.org/10.1177/156482651303400210

    Article  PubMed  Google Scholar 

  9. Mustafa TG, Monirujjaman MD, Zabeen S, Hossain B (2010) Effect of zinc supplementation on serum zinc level and micronucleus frequency in Bangladeshi adult females with poor socioeconomic status. AKMMC J 1:4–8

    Google Scholar 

  10. Mahmoud HM, Al-Tmimi DJ (2019) Evaluation of DNA damage in type 1 diabetes patients. J Clin Diagn Res 13(5):BC04–BC06

    Google Scholar 

  11. Al-Timimi DJ, Al-SharbattiSS A-NF (2005) Zinc deficiency among a healthy population in Baghdad, Iraq. Saudi Med J 26(11):1777–1781

    PubMed  Google Scholar 

  12. Ali AF, Altimimi DJ (2019) DNA damage and vitamin D status among type 2 diabetic patients. J Clin Diagn Res 13(5):21–24

    CAS  Google Scholar 

  13. Al-Timimi DJ, Al-Rubaii AY (2001) Current status of zinc in healthy and sick elderly. J Fac Med Baghdad 43(1):73–76

    Google Scholar 

  14. Al-Timimi DJ, Sulieman DM, Hussen KR (2014) Zinc status in type 2 diabetic patients: Relation to the progression of diabetic nephropathy. J Clin Diagn Res 8(11):CC04–CC08. https://doi.org/10.7860/JCDR/2014/10090.5082

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Ho E (2004) Zinc deficiency, DNA damage and cancer risk. J NutrBioch 15:572–578

    CAS  Google Scholar 

  16. Marreiro DN, Cruz KJ, Morais JB, Beserra JB, Severo JS, de Oliveira AS (2017) Zinc and oxidative stress: current mechanisms. Antioxidants (Basel) 6(2):24. https://doi.org/10.3390/antiox6020024

    Article  CAS  Google Scholar 

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Acknowledgments

We acknowledge the support of the staff of Duhok Diabetes Center, who provided the facilities for the interviews and the laboratory work.

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Authors and Affiliations

  1. Department of Medical Chemistry, College of Medicine, University of Duhok, Duhok, Kurdistan Region, Iraq

    Hivi M. Mahmoud & Dhia J. Al-Timimi

  2. Department of Medical Lab. Technology, Shekhan Technical College of Health, Duhok Polytechnic University, Duhok, Kurdistan Region, Iraq

    Ardawan F. Ali

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  1. Hivi M. Mahmoud
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  2. Ardawan F. Ali
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  3. Dhia J. Al-Timimi
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Correspondence to Hivi M. Mahmoud.

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Mahmoud, H.M., Ali, A.F. & Al-Timimi, D.J. Relationship Between Zinc Status and DNA Oxidative Damage in Patients with Type 2 Diabetes Mellitus. Biol Trace Elem Res 199, 1276–1279 (2021). https://doi.org/10.1007/s12011-020-02267-9

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  • DOI: https://doi.org/10.1007/s12011-020-02267-9

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