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Co-administration of Aluminum Sulfate and Propolis Regulates Matrix Metalloproteinases-2/9 Expression and Improves the Uterine Leiomyoma in Adult Rat Model

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Abstract

The aim of this study was to evaluate the effects of aluminum sulfate (alum) with propolis (PR) on uterine leiomyoma (UL) in rat model. One hundred and four female Wistar rats (180–200 g) were allocated into two main groups of control (Co, n = 8) and experiment (UL model [estradiol benzoate 200 μg/kg/IM twice/week/8 weeks] with/without treatment) defined in 13 subgroups with/without treatment with coil oil (UL + COi), PR (100 or 200 mg/kg) as UL + PR100 or 200, alum (35, 75 or 150 mg/Kg) as UL + AL 35, 75, or 150, and PR (100 mg/kg or 200) with alum (35, 75, or 150 mg/Kg) as UL + PR100 or 200 + AL35, 75, or 150. Subgroups received doses of therapeutics for 14 days (IP). In the end, rats were sacrificed, and the uteri were isolated for molecular and histopathological investigations. The myometrium thickness, collagen contents, and gene expression of MMP-2 and 9 increased significantly in experimental groups with/without treatment (P ˂ 0.05). PR administration (100 and 200 mg/kg) alone or with alum (35 and 75 mg/kg) significantly decreased myometrium collagen contents and the gene expression and protein concentration of MMP-2 and 9 compared with UL and UL + Coi subgroups (P ˂ 0.05). Alum (75 mg/kg) with PR (200 mg/kg) could improve UL features and reduce MMP-2 and 9 gene expression.

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This study was funded by Semnan University of Medical Sciences with a register number of A-10-65-7.

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Authors and Affiliations

  1. Abnormal Uterine Bleeding Research Center, Semnan University of Medical Sciences, Semnan, Iran

    Behpour Yousefi, Sam Zarbakhsh & Hoda Doustmohammadi

  2. Department of Anatomy, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran

    Behpour Yousefi, Mohammad Hasan Tabrizi Amjad & Hoda Doustmohammadi

  3. Department of Pathology, Semnan University of Medical Sciences, Semnan, Iran

    Vahid Semnani

  4. Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran

    Tahmineh Mokhtari & Mohammad Hasan Tabrizi Amjad

  5. Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran

    Sam Zarbakhsh

  6. Department of Medical Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mehdi Barati

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Yousefi, B., Semnani, V., Mokhtari, T. et al. Co-administration of Aluminum Sulfate and Propolis Regulates Matrix Metalloproteinases-2/9 Expression and Improves the Uterine Leiomyoma in Adult Rat Model. Biol Trace Elem Res 199, 1002–1012 (2021). https://doi.org/10.1007/s12011-020-02200-0

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