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Selenium Exerts Protective Effects Against Fluoride-Induced Apoptosis and Oxidative Stress and Altered the Expression of Bcl-2/Caspase Family

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Abstract

Fluoride is widely distributed in nature, and at high concentrations, it targets the kidney and especially proximal tubule epithelial cells. Selenium is a typical trace element beneficial to humans, and the role of selenium in the prevention and treatment of fluoride-induced organ damage is an important research topic. The purpose of this study was to investigate the possible protective effects of selenium against fluoride-induced oxidative stress and apoptosis in rat renal tubular epithelial cells. We showed that the activity of antioxidant enzymes (superoxide dismutase and glutathione peroxidase) and total antioxidant capacity were significantly reduced in NaF-treated normal rat kidney cells (NRK-52E), whereas the levels of nitrogen monoxide (NO) and malondialdehyde (MDA) were significantly increased. Moreover, the number of apoptotic cells, mRNA expression of Bax, Bad, caspase-3, caspase-8, and caspase-9, and protein expression of Bax were elevated, while mitochondrial membrane potential and the protein expression of Bcl-2 were reduced. Compared with the NaF group, pretreatment with selenium enhanced the activity of antioxidant enzymes, mitochondrial membrane potential, and protein expression of Bcl-2, while the levels of NO and MDA, number of apoptotic cells, mRNA expression of Bax, Bad, caspase-3, caspase-8, and caspase-9, and protein expression of Bax were decreased. In conclusion, selenium exerted remarkable protective effect against NaF-induced oxidative stress and apoptosis and altered the expression of Bcl-2/caspase family.

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Funding

This work was financed by grants from the China National Natural Science Foundation (No.31240009), the Special Funds for Experimental Animal Technologies in Shanxi Province of China (No.2012K02), and the Shanxi Scholarship Council of China (Grant No.2016-056).

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Authors and Affiliations

  1. Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science and Human Disease Animal Model, Shanxi Medical University, Road Xinjian 56, Taiyuan, 030001, China

    Jiping Gao, Xiaoru Yan, Yu Wang, Jianing Wei, Xiaotang Wang & Guohua Song

  2. Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, Taigu, 030801, China

    Xiaolin Tian & Xiaoyan Yan

  3. School of Public Health, Shanxi Medical University, Taiyuan, 030001, Shanxi, China

    Xiaolin Tian

  4. Mental Health Hosipital Affiliated to Shanxi Medical University, Street Nanshifang 55, Taiyuan City, 030001, Shanxi Province, China

    Guohua Song

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  1. Jiping Gao
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  3. Xiaoru Yan
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  4. Yu Wang
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Correspondence to Guohua Song.

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Gao, J., Tian, X., Yan, X. et al. Selenium Exerts Protective Effects Against Fluoride-Induced Apoptosis and Oxidative Stress and Altered the Expression of Bcl-2/Caspase Family. Biol Trace Elem Res 199, 682–692 (2021). https://doi.org/10.1007/s12011-020-02185-w

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