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Enterobacter sp. Mediated Synthesis of Biocompatible Nanostructured Iron-Polysaccharide Complexes: a Nutritional Supplement for Iron-Deficiency Anemia

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Abstract

FDA has approved iron oxide nanoparticles (IONs) coated with organic compounds as a safe material with less toxic effects compared with the naked metal ions and nanoparticles. In this study, the biological and physicochemical characteristics of a nanostructured iron-polysaccharide complexes (Nano-IPC) biosynthesized by Enterobacter sp. were evaluated. Furthermore, the serum biochemical parameters, tissue iron level, red blood cell parameters, and organ ferritin of rats were measured for investigating the effect of the Nano-IPCs in comparison with FeSO4 as a supplement for iron deficiency. The biosafety data demonstrated 35% increment of viability in Hep-G2 hepatocarcinoma cell lines when treated with nanoparticles (500 μg/mL) for 24 h. Besides, iron concentration in serum and tissue as well as the expression of ferritin L subunit in animals treated with the Nano-IPCs supplement were meaningfully higher than the FeSO4-supplemented and negative control animals. Moreover, the expression level of ferritin H subunit and biochemical factors remained similar to the negative control animals in the Nano-IPC-supplemented group. These results indicated that Nano-IPCs can be considered as a nontoxic supplement for patients carrying iron-deficiency anemia (IDA).

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Acknowledgments

This research is based on the Postdoc research proposal No. 18521 submitted to School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. A Grant from the Research Council of Shiraz University of Medical Sciences supported it.

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Authors and Affiliations

  1. Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 1583, 71345 Karafarin Street, Shiraz, Iran

    Sedigheh Kianpour, Manica Negahdaripour, Milad Mohkam & Younes Ghasemi

  2. Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Sedigheh Kianpour, Milad Mohkam & Seyyed Vahid Niknezhad

  3. Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Sedigheh Kianpour, Alireza Ebrahiminezhad, Reza Heidari, Manica Negahdaripour, Milad Mohkam, Seyyed Vahid Niknezhad & Younes Ghasemi

  4. Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran

    Alireza Ebrahiminezhad & Younes Ghasemi

  5. Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

    Bahman Khalvati

  6. Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014, Helsinki, Finland

    Mohammad-Ali Shahbazi

  7. Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, 4513956184, Iran

    Mohammad-Ali Shahbazi

  8. Department of Marine Chemistry, Faculty of Marine Science, Chabahar Maritime University, Chabahar, Iran

    Roghayyeh Aghaei

  9. School of Engineering, Faculty of Science and Engineering, University of Waikato, Hamilton, New Zealand

    Aydin Berenjian

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  1. Sedigheh Kianpour
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Kianpour, S., Ebrahiminezhad, A., Heidari, R. et al. Enterobacter sp. Mediated Synthesis of Biocompatible Nanostructured Iron-Polysaccharide Complexes: a Nutritional Supplement for Iron-Deficiency Anemia. Biol Trace Elem Res 198, 744–755 (2020). https://doi.org/10.1007/s12011-020-02101-2

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