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The Antagonistic Effects of Selenium Yeast (SeY) on Cadmium-Induced Inflammatory Factors and the Heat Shock Protein Expression Levels in Chicken Livers

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Abstract

Cadmium (Cd) is a ubiquitous toxic heavy metal in the natural environment that can cause multiple organ damage to the bodies of animals and humans. Selenium yeast (SeY) is a kind of organic selenium (Se) that has a very strong function against Cd-induced injury to tissues or organs. The aim of the current study was to investigate the roles of inflammatory factors and heat shock proteins (HSPs) in inflammatory injury triggered by Cd and to analyze the protective effects of SeY on Cd-induced damnification in the livers of chickens. Two hundred 120-day-old layers were randomly divided into four groups and raised on a conventional diet, or with Se (0.5 mg/kg SeY), Cd (150 mg/kg CdCl2), or Se + Cd (0.5 mg/kg SeY and 150 mg/kg CdCl2) in their basic diets for 120 days. Then, the liver histopathology, production of nitric oxide (NO), activity of inducible NO synthase (iNOS), and mRNA and protein expression levels of inflammatory factors (iNOS, NF-κB, TNF-α, and PTGE) and heat shock proteins (HSPs 27, 40, 60, 70, and 90) were examined. The results showed that exposure to Cd obviously increased Cd accumulation, NO production, iNOS activity, inflammatory factor, and HSP mRNA and protein expression levels and further caused an inflammatory response. Supplementation with SeY had an antagonistic effect on Cd-induced inflammatory injury in chicken livers. Thus, the present study suggests that SeY can be taken as a potential therapeutic for Cd-induced liver inflammatory injury in chickens.

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Funding

This study was supported by the Application Research Foundation of Xinjiang Production and Construction Corps (Project No. 2016AG010) and the National Natural Science Foundation of China (Project No. 31160521).

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  1. Yong Wang and Junfeng Liu contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science, Tarim University, Alar, 843300, Xinjiang Uygur Autonomous Region, China

    Yong Wang, Junfeng Liu, Rong Chen, Meng Qi, Dayong Tao & Shiwen Xu

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  1. Yong Wang
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Contributions

Shiwen Xu and Dayong Tao designed the research; Yong Wang and Junfeng Liu wrote the paper; Yong Wang, Junfeng Liu, and Meng Qi conducted the research; and Rong Chen analyzed the data. All the authors read and approved the final manuscript.

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Correspondence to Dayong Tao.

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The present study was approved by the ethical committee of Tarim University.

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Wang, Y., Liu, J., Chen, R. et al. The Antagonistic Effects of Selenium Yeast (SeY) on Cadmium-Induced Inflammatory Factors and the Heat Shock Protein Expression Levels in Chicken Livers. Biol Trace Elem Res 198, 260–268 (2020). https://doi.org/10.1007/s12011-020-02039-5

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