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Molybdenum Induces Mitochondrial Oxidative Damage in Kidney of Goats

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Abstract

The purpose of this study was to evaluate the effects of excessive molybdenum (Mo) on renal function and oxidative stress in goats. Twenty-seven healthy goats were randomly allotted in three groups and were fed deionized water to which sodium molybdate [(NH4)6Mo7O24·4H2O] was added at different doses of 0, 15, and 45 mg Mo/(kg·BW) for 50 days, respectively. The results indicated that white blood cell (WBC) counts were significantly increased (P < 0.05), while red blood cell (RBC) counts, hemoglobin (HGB), and mean corpuscular hemoglobin concentration (MCH) were tended to decrease with the increasing of the experimental period in high-Mo group compared with the control group. Besides, blood urea nitrogen (BUN) and creatinine (CREA) contents in serum were increased (P < 0.05) in both groups supplemented with molybdenum. Meanwhile, contents of copper (Cu) from the both experimental groups were significantly decreased (P < 0.05), while contents of zinc (Zn) and iron (Fe) were increased (P < 0.05) in serum. The contents of Cu were significantly increased (P < 0.05), while the contents of zinc (Zn) and iron (Fe) did not obviously change (P > 0.05) in the kidney. In addition, the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and catalase (CAT) significantly decreased (P < 0.05) in the mitochondria, whereas malondialdehyde (MDA) and nitric oxide synthase (NOS) expression significantly increased (P < 0.05). Collectively, these results indicated that excess Mo exposure could induce secondary Cu deficiency and oxidative stress in the kidney, which finally undermine the renal function of goats.

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Acknowledgments

The research was supported by the program of the National Natural Science Foundation (No. 31101863, Beijing, P.R. China), Training Plan for Young Scientists of Jiangxi Province (No. 2014BCB23040, Nanchang, P.R. China), and Education Department Science Foundation of Jiangxi Province (No. GJJ14294, Nanchang, P.R. China) to Huabin Cao.

Financial Support

Program of the National Natural Science Foundation ( 31101863) and Training Plan for Young Scientists of Jiangxi Province (2014BCB23040), these two are the equal first authors.

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Authors and Affiliations

  1. College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, P. R. China

    Jiapei Feng, Jian Chen, Chenghong Xing, Aimin Huang, Yu Zhuang, Fan Yang, Caiying Zhang, Guoliang Hu & Huabin Cao

  2. China Institute of Veterinary Drug Control (MOA Center for Veterinary Drug Evaluation), 8 Zhongguancun South Street Haidian District, Beijing, 100081, P. R. China

    Yaqing Mao

Authors
  1. Jiapei Feng
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  2. Jian Chen
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  3. Chenghong Xing
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  4. Aimin Huang
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  5. Yu Zhuang
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  6. Fan Yang
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  7. Caiying Zhang
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  8. Guoliang Hu
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  9. Yaqing Mao
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  10. Huabin Cao
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Contributions

Jiapei Feng performed experiments and written the original manuscript; Jian Chen completely finished the revised paper process; Chenghong Xing, Aiming Huang, and Yu Zhuang collected the data; Fan Yang, Caiying Zhang, and Yaqing Mao analyzed the data; and Huabin Cao supervised all of this work and designed this experiments. All authors have read the manuscript and agreed to submit it in its current form for consideration for publication in the Journal

Corresponding author

Correspondence to Huabin Cao.

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All experimental procedures complied with the criteria in Guide for the Care and Use of Laboratory Animals, and this study was approved by the institutional ethics committee.

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The authors declare that they have no conflict of interest.

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Jiapie Feng and Jian Chen are equal first author

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Feng, J., Chen, J., Xing, C. et al. Molybdenum Induces Mitochondrial Oxidative Damage in Kidney of Goats. Biol Trace Elem Res 197, 167–174 (2020). https://doi.org/10.1007/s12011-019-01991-1

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  • DOI: https://doi.org/10.1007/s12011-019-01991-1

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