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Manganese Chloride Exposure Causes Disorder of Energy Metabolism and Induces Oxidative Stress and Autophagy in Chicken Liver

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Abstract

Manganese (Mn) pollution is an important environmental problem because of the potential toxicity to human and animal health. However, the effects of Mn on energy metabolism and autophagy are not clear. Consequently, we examined the effects of excessive and chronic exposure to Mn on liver function, oxidative stress, respiratory chain complex activity, and autophagy in chicken liver. Our results indicated that the accumulation of Mn in the liver and levels of AST and ALT in the serum of the Mn-exposed group were significantly higher (P < 0.05) than those in the control group at 90 days; the activities of GSH-Px, SOD, CAT, Na+-K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, and respiratory chain complexes (I, II, III) in the Mn-exposed group were significantly decreased (P < 0.05) compared to the control group. However, the MDA content, NO content, iNOS activity, mRNA and protein levels of iNOS, and autophagy-related genes in the Mn-exposed group were significantly increased (P < 0.05) compared to the control group. In contrast, the mRNA level and protein expression of mTOR were significantly decreased (P < 0.05) compared to the control group. Furthermore, the characteristic autophagic vacuolar organelles were observed in the Mn-exposed group. These results suggested that excess Mn exposure can cause a disorder of energy metabolism by mitochondrial injury and induce oxidative stress and autophagy, which eventually lead to liver damage.

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Funding

This work is supported by the Heilongjiang Bayi Agricultural University Support Program for San Heng San Zong (TDJH201804) and Innovation Foundation of Heilongjiang Bayi Agricultural University (XA2016-03).

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  1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Sartu District, Daqing, 163319, China

    Jiancheng Jiang, Fengfeng Wang, Lina Wang, Jiawei Xiao & Donghua Guo

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  1. Jiancheng Jiang
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  2. Fengfeng Wang
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  3. Lina Wang
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  4. Jiawei Xiao
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  5. Donghua Guo
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Correspondence to Donghua Guo.

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All animal procedures were approved by the Animal Experiments Committee of the Heilongjiang Bayi Agricultural University (registration protocol 11/2016).

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Jiang, J., Wang, F., Wang, L. et al. Manganese Chloride Exposure Causes Disorder of Energy Metabolism and Induces Oxidative Stress and Autophagy in Chicken Liver. Biol Trace Elem Res 197, 254–261 (2020). https://doi.org/10.1007/s12011-019-01960-8

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