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Fluoride-Induced Alteration in the Diversity and Composition of Bacterial Microbiota in Mice Colon

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Abstract

Fluoride, as an environmental toxin, causes damage to intestinal mucosa. It may promote pathogen infection by increasing the intestinal mucosa permeability. In this study, the colonic fecal samples from the control group (C group, 0 mg/L NaF for 60 days) and the fluoride group (F group, 100 mg/L NaF for 60 days) were subjected to high-throughput 16S rRNA sequencing to verify the effects of fluoride on the colonic flora of animals. Results revealed a total of 253 operative taxonomical units (OTUs) in two groups, and 22 unique OTUs occurred in the F group. Fluoride increased the microbiota diversity and species richness of the colon. Concretely, the abundance of the Tenericutes was increased at the level of the phyla in the F group. In addition, in the F group, significant differences at the genus level were observed in Faecalibaculum, Alloprevotella, [Eubacterium]_xylanophilum_group, Prevotellaceae_UCG-001, and Ruminiclostridium_9, compared to the C group. Among them, except for the reduction in Faecalibaculum, the other four bacteria were increased in the F group. In summary, the intestinal microbial composition of mice was reconstituted by the presence of fluoride, and the significantly changing bacteria may partly account for the pathogenesis of fluoride-induced intestinal dysfunction.

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Funding

This work was supported by the Top Young Innovative Talents of Shanxi Agricultural University (Grant No. TYIT201408).

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Authors and Affiliations

  1. Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, Shanxi, China

    Rong Fu, Ruiyan Niu, Rui Li, Baijuan Yue, Xuhua Zhang, Qiqi Cao, Jundong Wang & Zilong Sun

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  1. Rong Fu
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  2. Ruiyan Niu
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  3. Rui Li
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  4. Baijuan Yue
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  8. Zilong Sun
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Correspondence to Zilong Sun.

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Table S1.

Operative taxonomical units (OTU) data in the control group (C group) and the fluoride group (F group). (XLSX 19 kb)

Table S2.

Relative abundance data of bacteria in the control group (C group) and the fluoride group (F group) at the phylum level. (XLSX 9 kb)

Table S3.

Relative abundance data of the top 20 bacteria in the control group (C group) and the fluoride group (F group) at the genus level. (XLSX 9 kb)

Table S4.

The top 50 genera correlation analysis using the SparCC method in the two groups. (XLSX 10 kb)

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Fu, R., Niu, R., Li, R. et al. Fluoride-Induced Alteration in the Diversity and Composition of Bacterial Microbiota in Mice Colon. Biol Trace Elem Res 196, 537–544 (2020). https://doi.org/10.1007/s12011-019-01942-w

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  • DOI: https://doi.org/10.1007/s12011-019-01942-w

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