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Multifarious Pharmacological Applications of Green Routed Eco-Friendly Iron Nanoparticles Synthesized by Streptomyces Sp. (SRT12)

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Abstract

A simple, eco-friendly, green routine co-precipitation method was experimented to synthesize iron nanoparticles (Fe-NPs) using the cell-free supernatant of actinobacteria. The biosynthesized nanoparticles were characterized by UV-Vis spectroscopy, X-ray diffractometer (XRD), energy-dispersive X-ray (EDX), scanning electron microscopy (SEM), atomic force microscopy (AFM), zeta potential analyser and Fourier transform infrared (FTIR) spectroscopy. The synthesized nanoparticles were crystalline, quasi-spherical in shape and their average size ranged from 65.0 to 86.7 nm. In our radical scavenging assays, the nanoparticles have revealed a strong antioxidant activity with respective standard ascorbic acid. The nanoparticles also exhibited a wide bactericidal action on pathogens namely Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Shigella flexneri and Escherichia coli. At 75 μg/ml concentration, the nanoparticles showed the highest inhibition against S. aureus (16.2 ± 0.45 mm), the lowest zone of inhibition was seen against K. pneumoniae (12.3 ± 0.50 mm) and moderate inhibition on other strains. Further, its cytotoxicity was seen as effective against DU145 and PC3 cells. The morphological changes caused in the prostate cell lines due to antiproliferative effect were observed through DAPI and AO/EB staining. This synthesis method specifies a new route for biosynthesis of Fe-NPs and the accomplished results illustrates that it can be used for a wide range of biomedical applications.

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Acknowledgements

The authors would like to thank Prof. B. Shanthi, Centralised Instrumentation and Service Laboratory (C.I.S.L), Department of Physics, Annamalai University, for providing facilities during the study period.

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Authors and Affiliations

  1. Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, 608 502, India

    Srinath Rajeswaran, Somasundaram Somasundaram Thirugnanasambandan & Naresh Kumar Dewangan

  2. Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India

    Rajesh Kannan Moorthy, Saravanan Kandasamy & Ravikumar Vilwanathan

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  1. Srinath Rajeswaran
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  2. Somasundaram Somasundaram Thirugnanasambandan
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Correspondence to Srinath Rajeswaran.

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Rajeswaran, S., Somasundaram Thirugnanasambandan, S., Dewangan, N.K. et al. Multifarious Pharmacological Applications of Green Routed Eco-Friendly Iron Nanoparticles Synthesized by Streptomyces Sp. (SRT12). Biol Trace Elem Res 194, 273–283 (2020). https://doi.org/10.1007/s12011-019-01777-5

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