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Effect of Endoxylanase and Iron Oxide Nanoparticles on Performance and Histopathological Features in Broilers

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A Correction to this article was published on 30 April 2020

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Abstract

Endoxylanase enzyme is used as poultry feed additive to degrade anti-nutritional factors like non-starch polysaccharides. Moreover, iron is one of the most important trace elements, and its deficiency can lead to various pathological conditions and stunted growth. In the current study, a combination of xylanase and iron nanoparticles is used to assess the overall effect on poultry growth. Endoxylanase enzyme was obtained from Aspergillus awamori SAIB-17 (identified on the basis of internal transcribed spacer (ITS) sequence analysis). The characterization of purified endoxylanase revealed that the Km and Vmax were 0.25 g/ml and 833.33 nkat/ml/s, respectively. Effect of pH and temperature showed that pH 4.5 and temperature 45 °C was best for enzyme activity. Iron nanoparticles were synthesized by co-precipitation of ferric chloride and ferrous chloride. Characterization of nanoparticles using X-ray diffraction (XRD) and SEM revealed that the mean diameter of synthesized iron nanoparticles was around 50 nm. These nanoparticles have no inhibitory effect on endoxylanase up to concentration of 20 ppm. Iron oxide nanoparticles along with endoxylanase were used as additives in different concentrations and were fed to the groups of broiler chicks. It was observed that the group fed with 40 nkat of endoxylanase and 15 ppm of iron nanoparticles showed 54.5% (2010 ± 103.58) more weight gain by the fifth week as compared with the control group. The iron analysis in the muscles showed no increase in iron concentration while histopathology slides showed no morphological changes in liver cells. The combination of iron oxide nanoparticles and xylanase proved to have great potential to be used in poultry feed for large-scale meat production without any toxicological effects.

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Change history

  • 30 April 2020

    The original version of this article unfortunately contained a mistake. The name of “Muhammad Talha Yasin” is now corrected in the author group of this article.

References

  1. Song L, Tsang A, Sylvestre M (2015) Engineering a thermostable fungal GH10 xylanase, importance of N-terminal amino acids. Biotechnol Bioeng 112(6):1081–1091

    Article  CAS  PubMed  Google Scholar 

  2. Zhang L, Xu J, Lei L, Jiang Y, Gao F, Zhou GH (2014) Effects of xylanase supplementation on growth performance, nutrient digestibility and non-starch polysaccharide degradation in different sections of the gastrointestinal tract of broilers fed wheat-based diets. Asian Australas J Anim Sci 27(6):855

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Taschetto D, Vieira SL, Angel CR, Stefanello C, Kindlein L, Ebbing MA, Simões CT (2017) Iron requirements of broiler breeder hens. Poult Sci 96(11):3920–3927

    Article  CAS  PubMed  Google Scholar 

  4. Nikonov IN, Folmanis YG, Folmanis GE, Kovalenko LV, Laptev GY, Egorov IA, Tananaev IG (2011) Iron nanoparticles as a food additive for poultry. In Doklady Biological Sciences (Vol. 440, No. 1, pp. 328–331). MAIK Nauka/Interperiodica

  5. Blair R (2018) Nutrition and feeding of organic poultry. CABI.

  6. Dubchak S, Ogar A, Mietelski JW, Turnau K (2010) Influence of silver and titanium nanoparticles on arbuscular mycorrhiza colonization and accumulation of radiocaesium in Helianthus annuus. Span J Agric Res 8(S1):103–108

    Article  Google Scholar 

  7. Iravani S, Korbekandi H, Mirmohammadi SV, Zolfaghari B (2014) Synthesis of silver nanoparticles: chemical, physical and biological methods. Res Pharm Sci 9(6):385

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Gupta AK, Naregalkar RR, Vaidya VD, Gupta M (2007) Recent advances on surface engineering of magnetic iron oxide nanoparticles and their biomedical applications. Nanomedicine 2(1):23–39

  9. Mabey D, Peeling RW, Ustianowski A, Perkins MD (2004) Tropical infectious diseases: diagnostics for the developing world. Nat Rev Microbiol 2(3):231

    Article  CAS  PubMed  Google Scholar 

  10. Hu Y, Jensen JO, Zhang W, Cleemann LN, Xing W, Bjerrum NJ, Li Q (2014) Hollow spheres of iron carbide nanoparticles encased in graphitic layers as oxygen reduction catalysts. Angew Chem Int Ed 53(14):3675–3679

    Article  CAS  Google Scholar 

  11. Zanganeh S, Hutter G, Spitler R, Lenkov O, Mahmoudi M, Shaw A et al (2016) Iron oxide nanoparticles inhibit tumour growth by inducing pro-inflammatory macrophage polarization in tumour tissues. Nat Nanotechnol 11(11):986

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Nyongesa BW, Okoth S, Ayugi V (2015) Identification key for Aspergillus species isolated from maize and soil of Nandi County, Kenya. Adv Microbiol 5(04):205

    Article  Google Scholar 

  13. Melchers WJ, Verweij PE, Van den Hurk P, Van Belkum A, De Pauw BE, Hoogkamp-Korstanje JA, Meis JF (1994) General primer-mediated PCR for detection of Aspergillus species. J Clin Microbiol 32(7):1710–1717

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Bokhari SAI, Latif F, Rajoka MI (2009) Purification and characterization of xylanases from Thermomyces lanuginosus and its mutant derivative possessing novel kinetic and thermodynamic properties. World J Microbiol Biotechnol 25(3):493–502

    Article  CAS  Google Scholar 

  15. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254

    Article  CAS  PubMed  Google Scholar 

  16. Bokhari SAI, Latif F, Rajoka MI (2008) Kinetics of high-level of β-glucosidase production by a 2-deoxyglucose-resistant mutant of Humicola lanuginosa in submerged fermentation. Braz J Microbiol 39:724–733

    Article  PubMed  PubMed Central  Google Scholar 

  17. Dalagnol LM, Silveira VC, da Silva HB, Manfroi V, Rodrigues RC (2017) Improvement of pectinase, xylanase and cellulase activities by ultrasound: effects on enzymes and substrates, kinetics and thermodynamic parameters. Process Biochem 61:80–87

    Article  CAS  Google Scholar 

  18. Karaagac O, Kockar H (2012) Effect of synthesis parameters on the properties of superparamagnetic iron oxide nanoparticles. J Supercond Nov Magn 25(8):2777–2781

    Article  CAS  Google Scholar 

  19. Bedford MR, Partridge GG (eds) (2010) Enzymes in farm animal nutrition. CABI, Wallingford.

  20. Bokhari SAI, Rajoka MI, Javed A, Rehman S u, Rehman I u, Latif F (2010) Novel thermodynamics of xylanase formation by a 2-deoxy-d-glucose resistant mutant of Thermomyces lanuginosus and its xylanase potential for biobleachability. Bioresour Technol 101:2800–2808

    Article  CAS  PubMed  Google Scholar 

  21. Saritha M, Singh S, Tiwari R, Goel R, Nain L (2016) Do cultural conditions induce differential protein expression: profiling of extracellular proteome of Aspergillus terreus CM20. Microbiol Res 192:73–83

    Article  CAS  Google Scholar 

  22. Saha SP, Ghosh S (2014) Optimization of xylanase production by Penicillium citrinum xym2 and application in saccharification of agro-residues. Biocatal Agric Biotechnol 3(4):188–196

    Article  Google Scholar 

  23. Javed U, Aman A, Qader SAU (2017) Utilization of corncob xylan as a sole carbon source for the biosynthesis of endo-1, 4-β xylanase from Aspergillus niger KIBGE-IB36. Bioresources and. Bioprocessing 4(1):19

    Article  Google Scholar 

  24. Jin N, Ma S, Liu Y, Yi X, He R, Xu H, ... Cao Y (2012) Thermophilic xylanase production by Aspergillus niger in solid state fermentation using wheat straw and corn cob. Afr J Microbiol Res 6(10):2387–2239

  25. Cunha L, Martarello R, Souza PMD, Freitas MMD, Barros KVG, Ferreira Filho EX, Magalhães PO (2018) Optimization of xylanase production from Aspergillus foetidus in soybean residue. Enzyme Res 2018. https://doi.org/10.1155/2018/6597017

  26. He H, Qin Y, Li N, Chen G, Liang Z (2015) Purification and characterization of a thermostable hypothetical xylanase from Aspergillus oryzae HML366. Appl Biochem Biotechnol 175(6):3148–3161

    Article  CAS  PubMed  Google Scholar 

  27. Li C, Li J, Wang R, Li X, Li J, Deng C, Wu M (2018) Substituting both the N-terminal and “cord” regions of a xylanase from Aspergillus oryzae to improve its temperature characteristics. Appl Biochem Biotechnol 185(4):1044–1059

    Article  CAS  PubMed  Google Scholar 

  28. Donnik IM (2017) Research of opportunities for using iron nanoparticles and amino acids in poultry nutrition. Int J 13(40):124–131

    Google Scholar 

  29. Saleh AA, El-Magd MA (2018) Beneficial effects of dietary silver nanoparticles and silver nitrate on broiler nutrition. Environ Sci Pollut Res 25(27):27,031–27,038

    Article  CAS  Google Scholar 

  30. Liu WC, Kim IH (2016) Effects of dietary xylanase supplementation on performance and functional digestive parameters in broilers fed wheat-based diets. Poult Sci 96(3):566–573

    Article  Google Scholar 

  31. Liu HH, Wang JW, Zhang RP, Chen X, Yu HY, Jin HB, ... He H (2012) In ovo feeding of IGF-1 to ducks influences neonatal skeletal muscle hypertrophy and muscle mass growth upon satellite cell activation. J Cell Physiol 227(4), 1465–1475.

  32. Sáiz MP, Martí MT, Mitjavila MT, Planas J (1993) Iron absorption by small intestine of chickens. Biol Trace Elem Res 36(1):7–14

    Article  PubMed  Google Scholar 

  33. Srinivasan V, Bhavan PS, Rajkumar G, Satgurunathan T, Muralisankar T (2016) Effects of dietary iron oxide nanoparticles on the growth performance, biochemical constituents and physiological stress responses of the giant freshwater prawn Macrobrachium rosenbergii post-larvae. In J Fish Aquat Stud 4(2):170–182

    Google Scholar 

  34. Behera T, Swain P, Rangacharulu PV, Samanta M (2014) Nano-Fe as feed additive improves the hematological and immunological parameters of fish, Labeo rohita H. Appl Nanosci 4(6):687–694

    Article  CAS  Google Scholar 

  35. Jain TK, Reddy MK, Morales MA, Leslie-Pelecky DL, Labhasetwar V (2008) Biodistribution, clearance, and biocompatibility of iron oxide magnetic nanoparticles in rats. Mol Pharm 5(2):316–327

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We are highly thankful to National Agriculture Research Center for providing facility for poultry experiments. We are also thankful to the Department of Physics, International Islamic University, for their support.

Funding

The first part of the research including identification of strain and production of xylanase enzyme was funded by HEC NRPU project no. 4753. The second part of the project including production and characterization of nanoparticles and application in poultry feed was funded by IIU funded project.

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Authors and Affiliations

  1. Department of Bioinformatics and Biotechnology, International Islamic University, Islamabad, Pakistan

    Hamza Rehman, Muzamal Akram, Mubin Mustafa Kiyani, Talha Yaseen, Abdul Ghani, Zafar Mahmood Khalid & Syed Ali Imran Bokhari

  2. Riphah College of Rehabilitation Sciences, Riphah International University, Islamabad, Pakistan

    Mubin Mustafa Kiyani

  3. Department of Physics, Quaid-i-Azam University, Islamabad, Pakistan

    Javed Iqbal Saggu

  4. Department of Pathology, Northern Border University, Arar, Saudi Arabia

    Syed Sajid Hussain Shah

Authors
  1. Hamza Rehman
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  2. Muzamal Akram
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  4. Talha Yaseen
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  5. Abdul Ghani
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  6. Javed Iqbal Saggu
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  7. Syed Sajid Hussain Shah
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  8. Zafar Mahmood Khalid
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  9. Syed Ali Imran Bokhari
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Correspondence to Hamza Rehman.

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The animal studies were conducted as per approved guidelines from the research ethical committee of International Islamic University, Islamabad, Pakistan (Letter No. IIU(BI&BT)/FBAS-IBBC-2017-1).

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The authors declare that they have no conflict of interest.

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Rehman, H., Akram, M., Kiyani, M.M. et al. Effect of Endoxylanase and Iron Oxide Nanoparticles on Performance and Histopathological Features in Broilers. Biol Trace Elem Res 193, 524–535 (2020). https://doi.org/10.1007/s12011-019-01737-z

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