The primary aim of our study was to determine the influence of taking chromium plus carnitine on insulin resistance, with a secondary objective of evaluating the influences on lipid profiles and weight loss in overweight subjects with polycystic ovary syndrome (PCOS). In a 12-week randomized, double-blind, placebo-controlled clinical trial, 54 overweight women were randomly assigned to receive either supplements (200 μg/day chromium picolinate plus 1000 mg/day carnitine) or placebo (27/each group). Chromium and carnitine co-supplementation decreased weight (− 3.6 ± 1.8 vs. − 1.0 ± 0.7 kg, P < 0.001), BMI (− 1.3 ± 0.7 vs. − 0.3 ± 0.3 kg/m2, P < 0.001), fasting plasma glucose (FPG) (− 5.1 ± 6.0 vs. − 1.1 ± 4.9 mg/dL, P = 0.01), insulin (− 2.0 ± 1.4 vs. − 0.2 ± 1.2 μIU/mL, P < 0.001), insulin resistance (− 0.5 ± 0.4 vs. − 0.04 ± 0.3, P < 0.001), triglycerides (− 18.0 ± 25.2 vs. + 5.5 ± 14.4 mg/dL, P < 0.001), total (− 17.0 ± 20.3 vs. + 3.6 ± 12.0 mg/dL, P < 0.001), and LDL cholesterol (− 13.3 ± 19.2 vs. + 1.4 ± 13.3 mg/dL, P = 0.002), and elevated insulin sensitivity (+ 0.007 ± 0.005 vs. + 0.002 ± 0.005, P < 0.001). In addition, co-supplementation upregulated peroxisome proliferator-activated receptor gamma (P = 0.02) and low-density lipoprotein receptor expression (P = 0.02). Overall, chromium and carnitine co-supplementation for 12 weeks to overweight women with PCOS had beneficial effects on body weight, glycemic control, lipid profiles except HDL cholesterol levels, and gene expression of PPAR-γ and LDLR. Clinical trial registration number: http://www.irct.ir: IRCT20170513033941N38.
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Polak K, Czyzyk A, Simoncini T, Meczekalski B (2017) New markers of insulin resistance in polycystic ovary syndrome. J Endocrinol Investig 40:1–8. https://doi.org/10.1007/s40618-016-0523-8
Azziz R, Carmina E, Chen Z, Dunaif A, Laven JSE, Legro RS, Lizneva D, Natterson-Horowtiz B, Teede HJ, Yildiz BO (2016) Polycystic ovary syndrome. Nat Rev Dis Primers 2:16057. https://doi.org/10.1038/nrdp.2016.57
Harris HR, Terry KL (2016) Polycystic ovary syndrome and risk of endometrial, ovarian, and breast cancer: a systematic review. Fertil Res Pract 2:14. https://doi.org/10.1186/s40738-016-0029-2
Condorelli RA, Calogero AE, Di Mauro M et al (2018) Androgen excess and metabolic disorders in women with PCOS: beyond the body mass index. J Endocrinol Investig 41:383–388
Goodman NF, Cobin RH, Futterweit W, Glueck JS, Legro RS, Carmina E (2015) American Association of Clinical Endocrinologists, American College of Endocrinology, and Androgen Excess and PCOS Society disease state clinical review: guide to the best practices in the evaluation and treatment of polycystic ovary syndrome - part 2. Endocr Pract 21:1415–1426
Wild RA, Rizzo M, Clifton S, Carmina E (2011) Lipid levels in polycystic ovary syndrome: systematic review and meta-analysis. Fertil Steril 95:1073–1079.e1071-1011
Baranova A, Tran TP, Afendy A, Wang L, Shamsaddini A, Mehta R, Chandhoke V, Birerdinc A, Younossi ZM (2013) Molecular signature of adipose tissue in patients with both non-alcoholic fatty liver disease (NAFLD) and polycystic ovarian syndrome (PCOS). J Transl Med 11:133. https://doi.org/10.1186/1479-5876-11-133
Xue Y, Xu P, Xue K, Duan X, Cao J, Luan T, Li Q, Gu L (2017) Effect of vitamin D on biochemical parameters in polycystic ovary syndrome women: a meta-analysis. Arch Gynecol Obstet 295:487–496
Foroozanfard F, Jamilian M, Jafari Z et al (2015) Effects of zinc supplementation on markers of insulin resistance and lipid profiles in women with polycystic ovary syndrome: a randomized, double-blind, placebo-controlled trial. Exp Clin Endocrinol Diabetes 123:215–220
Ringseis R, Keller J, Eder K (2012) Role of carnitine in the regulation of glucose homeostasis and insulin sensitivity: evidence from in vivo and in vitro studies with carnitine supplementation and carnitine deficiency. Eur J Nutr 51:1–18
Hua Y, Clark S, Ren J, Sreejayan N (2012) Molecular mechanisms of chromium in alleviating insulin resistance. J Nutr Biochem 23:313–319
Suksomboon N, Poolsup N, Yuwanakorn A (2014) Systematic review and meta-analysis of the efficacy and safety of chromium supplementation in diabetes. J Clin Pharm Ther 39:292–306
Asadi M, Rahimlou M, Shishehbor F, Mansoori A (2019) The effect of l-carnitine supplementation on lipid profile and glycaemic control in adults with cardiovascular risk factors: a systematic review and meta-analysis of randomized controlled clinical trials. Clin Nutr. https://doi.org/10.1016/j.clnu.2019.01.020
Cha YS (2008) Effects of L-carnitine on obesity, diabetes, and as an ergogenic aid. Asia Pac J Clin Nutr 17(Suppl 1):306–308
Jamilian M, Asemi Z (2015) Chromium supplementation and the effects on metabolic status in women with polycystic ovary syndrome: a randomized, double-blind, placebo-controlled trial. Ann Nutr Metab 67:42–48
Komorowski J, Juturu V (2005) Chromium supplementation does not improve glucose tolerance, insulin sensitivity, or lipid profile: a randomized, placebo-controlled, double-blind trial of supplementation in subjects with impaired glucose tolerance. Diabetes Care 28:712–713
Lee BJ, Lin JS, Lin YC, Lin PT (2016) Effects of L-carnitine supplementation on lipid profiles in patients with coronary artery disease. Lipids Health Dis 15:107. https://doi.org/10.1186/s12944-016-0277-5
Samimi M, Jamilian M, Ebrahimi FA, Rahimi M, Tajbakhsh B, Asemi Z (2016) Oral carnitine supplementation reduces body weight and insulin resistance in women with polycystic ovary syndrome: a randomized, double-blind, placebo-controlled trial. Clin Endocrinol 84:851–857
Zhou B, Wang H, Luo G, Niu R, Wang J (2013) Effect of dietary yeast chromium and L-carnitine on lipid metabolism of sheep. Biol Trace Elem Res 155:221–227
Wang J, Du R, Qin J et al (2003) Effect of yeast chromium and L-carnitine on lipid metabolism of broiler chickens. Asian Austral J Anim 16:1809–1815
Tian H, Guo X, Wang X et al (2013) Chromium picolinate supplementation for overweight or obese adults. Cochrane Database Syst Rev:Cd010063. https://doi.org/10.1002/14651858.CD010063.pub2
Ainsworth BE, Haskell WL, Whitt MC et al (2000) Compendium of physical activities: an update of activity codes and MET intensities. Med Sci Sports Exerc 32:S498–S504
Pisprasert V, Ingram KH, Lopez-Davila MF, Munoz AJ, Garvey WT (2013) Limitations in the use of indices using glucose and insulin levels to predict insulin sensitivity: impact of race and gender and superiority of the indices derived from oral glucose tolerance test in African Americans. Diabetes Care 36:845–853
Pooyandjoo M, Nouhi M, Shab-Bidar S, Djafarian K, Olyaeemanesh A (2016) The effect of (L-)carnitine on weight loss in adults: a systematic review and meta-analysis of randomized controlled trials. Obes Rev 17:970–976
Onakpoya I, Posadzki P, Ernst E (2013) Chromium supplementation in overweight and obesity: a systematic review and meta-analysis of randomized clinical trials. Obes Rev 14:496–507
Fazelian S, Rouhani MH, Bank SS, Amani R (2017) Chromium supplementation and polycystic ovary syndrome: a systematic review and meta-analysis. J Trace Elem Med Biol 42:92–96
Tang XL, Sun Z, Gong L (2018) Chromium supplementation in women with polycystic ovary syndrome: systematic review and meta-analysis. J Obstet Gynaecol Res 44:134–143
Sundaram B, Singhal K, Sandhir R (2012) Ameliorating effect of chromium administration on hepatic glucose metabolism in streptozotocin-induced experimental diabetes. Biofactors 38:59–68
Xu Y, Jiang W, Chen G et al (2017) L-carnitine treatment of insulin resistance: a systematic review and meta-analysis. Adv Clin Exp Med 26:333–338
Vidal-Casariego A, Burgos-Pelaez R, Martinez-Faedo C et al (2013) Metabolic effects of L-carnitine on type 2 diabetes mellitus: systematic review and meta-analysis. Exp Clin Endocrinol Diabetes 121:234–238
Derosa G, Maffioli P, Ferrari I, D’Angelo A, Fogari E, Palumbo I, Randazzo S, Cicero AFG (2011) Comparison between orlistat plus l-carnitine and orlistat alone on inflammation parameters in obese diabetic patients. Fundam Clin Pharmacol 25:642–651
Derosa G, Cicero AF, Gaddi A, Mugellini A, Ciccarelli L, Fogari R (2003) The effect of L-carnitine on plasma lipoprotein(a) levels in hypercholesterolemic patients with type 2 diabetes mellitus. Clin Ther 25:1429–1439
Sahin K, Tuzcu M, Orhan C, Sahin N, Kucuk O, Ozercan IH, Juturu V, Komorowski JR (2013) Anti-diabetic activity of chromium picolinate and biotin in rats with type 2 diabetes induced by high-fat diet and streptozotocin. Br J Nutr 110:197–205
Pala R, Genc E, Tuzcu M, Orhan C, Sahin N, Er B, Cinar V, Sahin K (2018) L-carnitine supplementation increases expression of PPAR-gamma and glucose transporters in skeletal muscle of chronically and acutely exercised rats. Cell Mol Biol (Noisy-le-grand) 64:1–6
Zheng JL, Luo Z, Zhuo MQ, Pan YX, Song YF, Hu W, Chen QL (2014) Dietary L-carnitine supplementation increases lipid deposition in the liver and muscle of yellow catfish (Pelteobagrus fulvidraco) through changes in lipid metabolism. Br J Nutr 112:698–708
Caviglia D, Scarabelli L, Palmero S (2004) Effects of carnitines on rat sertoli cell protein metabolism. Horm Metab Res 36:221–225
Matsusue K, Peters JM, Gonzalez FJ (2004) PPARbeta/delta potentiates PPARgamma-stimulated adipocyte differentiation. FASEB J 18:1477–1479
Sharma AK, Bharti S, Ojha S et al (2011) Up-regulation of PPARgamma, heat shock protein-27 and -72 by naringin attenuates insulin resistance, beta-cell dysfunction, hepatic steatosis and kidney damage in a rat model of type 2 diabetes. Br J Nutr 106:1713–1723
Fernyhough ME, Okine E, Hausman G, Vierck JL, Dodson MV (2007) PPARgamma and GLUT-4 expression as developmental regulators/markers for preadipocyte differentiation into an adipocyte. Domest Anim Endocrinol 33:367–378
Jamilian M, Zadeh Modarres S, Amiri Siavashani M, Karimi M, Mafi A, Ostadmohammadi V, Asemi Z (2018) The influences of chromium supplementation on glycemic control, markers of cardio-metabolic risk, and oxidative stress in infertile polycystic ovary syndrome women candidate for in vitro fertilization: a randomized, double-blind, placebo-controlled trial. Biol Trace Elem Res 185:48–55
Paiva AN, Lima JG, Medeiros AC et al (2015) Beneficial effects of oral chromium picolinate supplementation on glycemic control in patients with type 2 diabetes: a randomized clinical study. J Trace Elem Med Biol 32:66–72
Malaguarnera M, Vacante M, Avitabile T, Malaguarnera M, Cammalleri L, Motta M (2009) L-carnitine supplementation reduces oxidized LDL cholesterol in patients with diabetes. Am J Clin Nutr 89:71–76
Strijbis K, Vaz FM, Distel B (2010) Enzymology of the carnitine biosynthesis pathway. IUBMB Life 62:357–362
Lee H-G, Yin J-L, Xu C-X, Hong ZS, Lee ZH, Jin YC, Choi CW, Lee DH, Kim KH, Choi YJ (2011) Effects of the combination of glucose, chromium picolinate, and vitamin c on lipid metabolism in steers. Asian Australas J Anim Sci 24:1674–1680
Patalay M, Lofgren IE, Freake HC, Koo SI, Fernandez ML (2005) The lowering of plasma lipids following a weight reduction program is related to increased expression of the LDL receptor and lipoprotein lipase. J Nutr 135:735–739
Woodworth JC, Tokach MD, Nelssen JL, Goodband RD, Dritz SS, Koo SI, Minton JE, Owen KQ (2007) Influence of dietary L-carnitine and chromium picolinate on blood hormones and metabolites of gestating sows fed one meal per day. J Anim Sci 85:2524–2537
The authors would like to thank the staff of Taleghani Clinic (Arak, Iran) for their assistance in this project.
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Jamilian, M., Foroozanfard, F., Kavossian, E. et al. Effects of Chromium and Carnitine Co-supplementation on Body Weight and Metabolic Profiles in Overweight and Obese Women with Polycystic Ovary Syndrome: a Randomized, Double-Blind, Placebo-Controlled Trial. Biol Trace Elem Res 193, 334–341 (2020). https://doi.org/10.1007/s12011-019-01720-8
- Body weight
- Metabolic profiles
- Polycystic ovary syndrome