Skip to main content
SpringerLink
Log in

Effects of Fungal Polysaccharide on Oxidative Damage and TLR4 Pathway to the Central Immune Organs in Cadmium Intoxication in Chickens

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

Cadmium (Cd) can cause animal organism damage, but there have been few studies on the damage of cadmium to the immune organs of birds. Most fungal polysaccharide has antioxidant and immunomodulatory effects. The experimental study investigated the effects of fungal polysaccharide (Agaricus blazei Murill polysaccharide and Ganoderma luciduccharide) on the oxidative damage of central immune organs (thymus and bursa of Fabricius) and on the Toll-like receptor 4 (TLR4) pathway in cadmium-poisoned chickens. The results showed that Agaricus blazei polysaccharide and Ganoderma lucidum polysaccharide can reduce cadmium content, TLR4 expression, inflammatory factor (IL-1β, IL-6, TNF-α) content, and lipid peroxidation product MDA content and increase the activity of antioxidant enzymes SOD and GSH-Px in thymus and bursa of cadmium poisoning chickens. Ganoderma lucidum polysaccharide could decrease the expression of TLR4, IL-1β, and IL-6 in cadmium poisoning peripheral blood lymphocytes of chicken, and TLR4 inhibitor had the same effect. The results demonstrated the protective effects of Agaricus blazei Murill polysaccharide and Ganoderma lucidum polysaccharides on the damage of the central immune organs of chickens caused by cadmium poisoning were closely related to the TLR4 signaling pathway and oxidative stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Burger J (2008) Assessment and management of risk to wildlife from cadmium. Sci Total Environ 389(1):37–45

    Article  CAS  PubMed  Google Scholar 

  2. Patar A, Giri A, Boro F, Bhuyan K, Singha U, Giri S (2016) Cadmium pollution and amphibians - studies in tadpoles of Rana limnocharis. Chemosphere 144:1043–1049

    Article  CAS  PubMed  Google Scholar 

  3. Huo J, Dong A, Yan J, Wang L, Ma C, Lee S (2017) Cadmium toxicokinetics in the freshwater turtle, Chinemys reevesii. Chemosphere 182:392–398

    Article  CAS  PubMed  Google Scholar 

  4. Brzóska MM, Majewska K, Moniuszkojakoniuk J (2004) Mineral status and mechanical properties of lumbar spine of female rats chronically exposed to various levels of cadmium. Bone 34(3):517–526

    Article  CAS  PubMed  Google Scholar 

  5. Marth E, Barth S, Jelovcan S (2000) Influence of cadmium on the immune system. Description of stimulating reactions. Cent Eur J Public Health 8(1):40–44

    CAS  PubMed  Google Scholar 

  6. Calabrese V, Cighetti R, Peri F (2015) Molecular simplification of lipid a structure: TLR4-modulating cationic and anionic amphiphiles. Mol Immunol 63(2):153–161

    Article  CAS  PubMed  Google Scholar 

  7. Pascuallucas M, Fernandezlizarbe S, Montesinos J, Guerri C (2014) LPS or ethanol triggers clathrin- and rafts/caveolae-dependent endocytosis of TLR4 in cortical astrocytes. J Neurochem 129(3):448–462

    Article  CAS  Google Scholar 

  8. Oblak A, Pohar J, Jerala R (2015) MD-2 determinants of nickel and cobalt-mediated activation of human TLR4. 10(3):e0120583

  9. Song SY, Bae CH, Choi YS, Kim YD (2016) Cadmium induces mucin 8 expression via Toll-like receptor 4-mediated extracellular signal related kinase 1/2 and p38 mitogen-activated protein kinase in human airway epithelial cells. Int Forum Allergy Rhinol 6(6):638–645

    Article  PubMed  Google Scholar 

  10. Oshiman K, Fujimiya Y, Ebina T, Suzuki I, Noji M (2002) Orally administered beta-1,6-D-polyglucose extracted from Agaricus blazei results in tumor regression in tumor-bearing mice. Planta Med 68(07):610–614

    Article  CAS  PubMed  Google Scholar 

  11. Liu Z, Ma X, Deng B, Huang Y, Bo R, Gao Z, Yu Y, Hu Y, Liu J, Wu Y (2015) Development of liposomal Ganoderma lucidum polysaccharide: formulation optimization and evaluation of its immunological activity. Carbohydr Polym 117:510–517

    Article  CAS  PubMed  Google Scholar 

  12. Shi M, Zhang Z, Yang Y (2013) Antioxidant and immunoregulatory activity of Ganoderma lucidum polysaccharide (GLP). Carbohydr Polym 95(1):200–206

    Article  CAS  PubMed  Google Scholar 

  13. Song Y, Zhang R, Wang H, Yan Y, Ming G (2017) Protective effect of Agaricus blazei polysaccharide against cadmium-induced damage on the testis of chicken. Biol Trace Elem Res:1–10

  14. Hu X, Zhang R, Xie Y, Wang H, Ge M (2016) The protective effects of polysaccharides from Agaricus blazei Murill against cadmium-induced oxidant stress and inflammatory damage in chicken livers. Biol Trace Elem Res 178(1):1–10

    Google Scholar 

  15. Daley T, Omoregie SN, Wright V, Omoruyi FO (2013) Effects of phytic acid and exercise on some serum analytes in rats orally exposed to diets supplemented with cadmium. Biol Trace Elem Res 151(3):400–405

    Article  CAS  PubMed  Google Scholar 

  16. Satarug S, Garrett SH, Sens MA, Sens DA (2011) Cadmium, environmental exposure, and health outcomes. Environ Health Perspect 16(5):182–190

    Google Scholar 

  17. Xie W, Lv A, Li R, Tang Z, Ma D, Huang X, Zhang R, Ge M (2017) Agaricus blazei Murill polysaccharides protect against cadmium-induced oxidative stress and inflammatory damage in chicken spleens. Biol Trace Elem Res (1):1–12

  18. Zhang R, Yu Q, Shi G, Rui L, Zhang W, Xia Z, Li G, Ming G (2017) chTLR4 pathway activation by Astragalus polysaccharide in bursa of Fabricius. BMC Vet Res 13(1):119

    Article  CAS  PubMed  Google Scholar 

  19. Shagirtha K, ., Muthumani M, ., S M Prabu (2011) Melatonin abrogates cadmium induced oxidative stress related neurotoxicity in rats. Eur Rev Med Pharmacol Sci 15 (9):1039, 1050

    CAS  PubMed  Google Scholar 

  20. Zhao P, Guo Y, Zhang W, Chai H, Xing H, Xing M (2017) Neurotoxicity induced by arsenic in Gallus Gallus: regulation of oxidative stress and heat shock protein response. Chemosphere 166:238–245

    Article  CAS  PubMed  Google Scholar 

  21. Jia S, Li F, Liu Y, Ren H, Gong G, Wang Y, Wu S (2013) Effects of extraction methods on the antioxidant activities of polysaccharides from Agaricus blazei Murrill. Int J Biol Macromol 62(11):66–69

    Article  CAS  PubMed  Google Scholar 

  22. Pan K, Jiang Q, Liu G, Miao X, Zhong D (2013) Optimization extraction of Ganoderma lucidum polysaccharides and its immunity and antioxidant activities. Int J Biol Macromol 55(2):301–306

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank the members of the Traditional Chinese Veterinary Medicine Laboratory at the College of Veterinary Medicine, Northeast Agricultural University.

Funding

This work was supported by the National Science Foundation of China (Grant No. 31272533).

Author information

Author notes

  1. Ruyue Li and Linan Zhang contributed equally to this work and should be considered as co-first authors.

Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China

    Ruyue Li, Linan Zhang, Zequn Tang, Tianqi Li, Guangxing Li, Ruili Zhang & Ming Ge

  2. Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, China

    Ruyue Li, Linan Zhang, Zequn Tang, Tianqi Li, Guangxing Li, Ruili Zhang & Ming Ge

Authors
  1. Ruyue Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Linan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zequn Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tianqi Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guangxing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ruili Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ming Ge
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Ruili Zhang or Ming Ge.

Ethics declarations

Ethical treatment of animals used in this study was approved by the Animal Welfare Committee protocol (#NEAU-2013-02-0252-11) at Northeast Agricultural University (Harbin, China).

Conflict of Interest

The authors declare that they have no conflict of interest.

Disclaimer

All authors have read the manuscript and agreed to submit it in its current form for consideration for publication in the journal.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, R., Zhang, L., Tang, Z. et al. Effects of Fungal Polysaccharide on Oxidative Damage and TLR4 Pathway to the Central Immune Organs in Cadmium Intoxication in Chickens. Biol Trace Elem Res 191, 464–473 (2019). https://doi.org/10.1007/s12011-018-1627-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-018-1627-0

Keywords

Search

Navigation