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Effect on Serum Parameters and Immune Responses of Carassius auratus gibelio Exposed to Dietary Lead and Bacillus subtilis

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Abstract

Lead (Pb), a heavy metal and an environmental stressor, may affect many physiological processes, including the serum index and the immune response. The aim of this study was to explore the toxic effects of Pb on the serum index and the immune response of Carassius auratus gibelio (C. gibelio) fed 0, 120, or 240 mg/kg Pb, and 109 cfu/g Bacillus subtilis (B. subtilis). After 15 and 30 days of dietary exposure, the serum indices and the immune responses of the fish were assessed. Dietary Pb exposure significantly affected various components of the serum index, including calcium, magnesium, glucose, cholesterol, total protein, glutamic-pyruvic transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH). However, sIgA activity in the gut increased significantly following B. subtilis supplementation. Notable changes were also observed in the expression levels of immune-related genes, including HSP70, IgM, HSP90, IL-1β, IL-6, and TNF-α. B. subtilis supplementation effectively attenuated the effects of dietary Pb exposure.

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Funding

This work was supported by the National Natural Sciences Foundational of China (no. 30972191) and the 948 Program from Ministry of Agriculture of China (no. 2014Z34).

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Authors and Affiliations

  1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China

    Yanan Cai, Yuwei Yin, Longtao Wang, Dongze Leng, Chenxia Ge, Abedin Abdallah & Yuehong Li

  2. Ministry of Education Laboratory of Animal Production and Quality Security, Jilin Agricultural University, Changchun, 130118, China

    Yanan Cai, Yuwei Yin, Longtao Wang, Dongze Leng, Chenxia Ge, Abedin Abdallah & Yuehong Li

Authors
  1. Yanan Cai
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  2. Yuwei Yin
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  3. Longtao Wang
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  5. Chenxia Ge
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  6. Abedin Abdallah
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  7. Yuehong Li
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Corresponding author

Correspondence to Yuehong Li.

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This study was approved by the Ethics Committee of Jilin Agricultural University with ID no. 20121008. All authors read this guide carefully. All subjects signed their informed consents before participation.

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The authors declare that they have no conflict of interest.

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Cai, Y., Yin, Y., Wang, L. et al. Effect on Serum Parameters and Immune Responses of Carassius auratus gibelio Exposed to Dietary Lead and Bacillus subtilis. Biol Trace Elem Res 190, 217–225 (2019). https://doi.org/10.1007/s12011-018-1544-2

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  • DOI: https://doi.org/10.1007/s12011-018-1544-2

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