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Heme and Non-heme Iron on Growth Performances, Blood Parameters, Tissue Mineral Concentration, and Intestinal Morphology of Weanling Pigs

  • Zhao Zhuo
  • Xiaonan Yu
  • Sisi Li
  • Shenglin Fang
  • Jie Feng
Article

Abstract

This experiment was conducted to evaluate the effects of heme and non-heme iron sources on growth performances, blood parameters, tissue mineral concentration, and intestinal morphology in weanling pigs. At 25 days of age, 32 newly weaned piglets (Duroc × Landrace × Yorkshire; 8.66 ± 0.59 kg) were allocated to one of the following dietary treatments: control group (basal diet with no extra iron addition), FeSO4 group (basal diet + 100 mg Fe/kg as FeSO4), Fe-Gly group (basal diet + 100 mg Fe/kg as Fe-Gly), and Heme group (basal diet + 100 mg Fe/kg as Heme). Each treatment had eight replicates and one pig per replicate. The experiment lasted for 28 days. The results showed that compared with basal diet, supplement with 100 mg/kg iron can increase ADG of the piglets, especially in the late experiment period (15~28 days). Heme significantly increased the a* value of longissimus dorsi muscle of piglets when compared with other iron sources (P < 0.05). The iron supplementations had no significant effect on hematological parameters, while Fe-Gly and heme increased pigs’ serum iron content on day 28 when compared with FeSO4 and basal diet (P < 0.05). The liver iron deposition in pigs fed Fe-Gly and heme was also higher than those fed FeSO4 or basal diet (P < 0.05). Besides, diet supplement with iron significantly increased villus height (P < 0.05) in duodenum and it had tendency to increase villus height and crypt depth ratio in duodenum (P = 0.095). In conclusion, iron supplementation in diets can improve piglet’s body iron state and intestinal development, but Fe-Gly and heme exhibited better bioavailability than traditional additive of FeSO4.

Keywords

Iron sources Growth performances Blood parameters Intestinal morphology 

Notes

Acknowledgements

This research was financially supported by the National Natural Sciences Foundation of China (No. 31472102 and 31772607) and the National Key Technologies R & D Program of China (No. 2016YFD0501201).

Compliance with Ethical Standards

The authors declare that there are no competing financial interests. All of the animal experiments were approved by the Animal Ethics Committee of Zhejiang University. The experimental procedures strictly followed the institutional and national guidelines for the care and use of animals.

References

  1. 1.
    Hentze MW, Muckenthaler MU, Andrews NC (2004) Balancing acts: molecular control of mammalian iron metabolism. Cell 117(3):285–297CrossRefPubMedGoogle Scholar
  2. 2.
    Steinbicker AU, Muckenthaler MU (2013) Out of balance—systemic iron homeostasis in iron-related disorders. Nutrients 5(8):3034–3061CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Núñez MT, Tapia V, Rojas A, Aguirre P, Gómez F, Nualart F (2009) Iron supply determines apical/basolateral membrane distribution of intestinal iron transporters DMT1 and ferroportin 1. Am J Physiol-Cell Physiol 298(3):C477–C485CrossRefPubMedGoogle Scholar
  4. 4.
    Meynard D, Babitt JL, Lin HY (2014) The liver: conductor of systemic iron balance. Blood 123(2):168–176CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Bovell-Benjamin AC, Viteri FE, Allen LH (2000) Iron absorption from ferrous bisglycinate and ferric trisglycinate in whole maize is regulated by iron status. Am J Clin Nutr 71(6):1563–1569CrossRefPubMedGoogle Scholar
  6. 6.
    Kegley EB, Spears JW, Flowers WL, Schoenherr WD (2002) Iron methionine as a source of iron for the neonatal pig. Nutr Res 22(10):1209–1217CrossRefGoogle Scholar
  7. 7.
    Ma WQ, Sun H, Zhou Y, Wu J, Feng J (2012) Effects of iron glycine chelate on growth, tissue mineral concentrations, fecal mineral excretion, and liver antioxidant enzyme activities in broilers. Biol Trace Elem Res 149(2):204–211CrossRefPubMedGoogle Scholar
  8. 8.
    Fang CL, Zhuo Z, Fang SL, Yue M, Feng J (2013) Iron sources on iron status and gene expression of iron related transporters in iron-deficient piglets. Anim Feed Sci Technol 182(1):121–125CrossRefGoogle Scholar
  9. 9.
    Zhuo Z, Fang S, Hu Q, Huang D, Feng J. (2016) Digital gene expression profiling analysis of duodenum transcriptomes in SD rats administered ferrous sulfate or ferrous glycine chelate by gavage. Sci Rep 6Google Scholar
  10. 10.
    Zhuo Z, Fang S, Yue M, Zhang Y, Feng J (2014) Kinetics absorption characteristics of ferrous glycinate in SD rats and its impact on the relevant transport protein. Biol Trace Elem Res 158(2):197–202CrossRefPubMedGoogle Scholar
  11. 11.
    Zimmermann MB, Hurrell RF (2007) Nutritional iron deficiency. Lancet 370(9586):511–520CrossRefPubMedGoogle Scholar
  12. 12.
    Hurrell R (2002) How to ensure adequate iron absorption from iron-fortified food. Nutr Rev 60(s7):S7–S15CrossRefPubMedGoogle Scholar
  13. 13.
    Zimmermann MB, Chaouki N, Hurrell RF (2005) Iron deficiency due to consumption of a habitual diet low in bioavailable iron: a longitudinal cohort study in Moroccan children. Am J Clin Nutr 81(1):115–121CrossRefPubMedGoogle Scholar
  14. 14.
    Shelton JL, Southern LL (2006) Effects of phytase addition with or without a trace mineral premix on growth performance, bone response variables, and tissue mineral concentrations in commercial broilers. J Appl Poult Res 15(1):94–102CrossRefGoogle Scholar
  15. 15.
    Hill GM, Miller ER, Whetter PA, Ullrey DE (1983) Concentration of minerals in tissues of pigs from dams fed different levels of dietary zinc. J Anim Sci 57(1):130–138CrossRefPubMedGoogle Scholar
  16. 16.
    Kwon CH, Lee CY, Han SJ, Kim SJ, Park BC, Jang I, Han JH (2014) Effects of dietary supplementation of lipid-encapsulated zinc oxide on colibacillosis, growth and intestinal morphology in weaned piglets challenged with enterotoxigenic Escherichia coli. Anim Sci J 85(8):805–813CrossRefPubMedGoogle Scholar
  17. 17.
    Song ZH, Xiao K, Ke YL, Jiao LF, Hu CH (2015) Zinc oxide influences mitogen-activated protein kinase and TGF-β1 signaling pathways, and enhances intestinal barrier integrity in weaned pigs. Innate Immun 21(4):341–348CrossRefPubMedGoogle Scholar
  18. 18.
    Svoboda M, Drabek J (2005) Iron deficiency in suckling piglets: etiology, clinical aspects and diagnosis. Folia Vet 49:104–111Google Scholar
  19. 19.
    Lipiński P, Starzyński RR, Canonne-Hergaux F, Tudek B, Oliński R, Kowalczyk P, ..., Woliński J (2010) Benefits and risks of iron supplementation in anemic neonatal pigs. Am J Pathol 177(3):1233–1243Google Scholar
  20. 20.
    Layrisse M, García-Casal MN, Solano L, Barón MA, Arguello F, Llovera D, ..., Tropper E (2000) Iron bioavailability in humans from breakfasts enriched with iron bis-glycine chelate, phytates and polyphenols. J Nutr 130(9):2195–2199Google Scholar
  21. 21.
    Ashmead SD (2001) The chemistry of ferrous bis-glycinate chelate. Arch Latinoam Nutr 51(1 Suppl 1):7–12PubMedGoogle Scholar
  22. 22.
    Anderson GJ, Frazer DM, McKie AT, Vulpe CD, Smith A (2005) Mechanisms of haem and non-haem iron absorption: lessons from inherited disorders of iron metabolism. Biometals 18(4):339–348CrossRefPubMedGoogle Scholar
  23. 23.
    Yu B, Huang WJ, Chiou PWS (2000) Bioavailability of iron from amino acid complex in weanling pigs. Anim Feed Sci Technol 86(1):39–52CrossRefGoogle Scholar
  24. 24.
    Victor I, Mary I (2012) Iron nutrition and anaemia in piglets: a review. J Vet Adv 2(6):261–265Google Scholar
  25. 25.
    Pu Y, Guo B, Liu D, Xiong H, Wang Y, Du H (2015) Iron supplementation attenuates the inflammatory status of anemic piglets by regulating hepcidin. Biol Trace Elem Res 167(1):28–35CrossRefPubMedGoogle Scholar
  26. 26.
    Wang J, Li D, Che L, Lin Y, Fang Z, Xu S, Wu D (2014) Influence of organic iron complex on sow reproductive performance and iron status of nursing pigs. Livest Sci 160:89–96CrossRefGoogle Scholar
  27. 27.
    Ventrella D, Dondi F, Barone F, Serafini F, Elmi A, Giunti M, Romagnoli N, Forni M, Bacci ML (2016) The biomedical piglet: establishing reference intervals for haematology and clinical chemistry parameters of two age groups with and without iron supplementation. BMC Vet Res 13(1):23CrossRefGoogle Scholar
  28. 28.
    Smith GC, Belk KE, Sofos JN, Tatum JD, Williams SN (2000) Economic implications of improved color stability in beef. Antioxidants in muscle foods: nutritional strategies to improve quality. Wiley, New York, pp 397–426Google Scholar
  29. 29.
    Olsson V, Pickova J (2005) The influence of production systems on meat quality, with emphasis on pork. Ambio 34(4):338–343CrossRefPubMedGoogle Scholar
  30. 30.
    Zhuo Z, Fang S, Yue M, Wang Y, Feng J (2013) Iron glycine chelate on meat color, iron status and myoglobin gene regulation of M. Longissimus dorsi in weaning pigs. Int J Agric Biol 15(5):983–987Google Scholar
  31. 31.
    Staroń R, Lipiński P, Lenartowicz M, Bednarz A, Gajowiak A, Smuda E, Krzeptowski W, Pieszka M, Korolonek T, Hamza I, Swinkels DW, van Swelm RPL, Starzyński RR (2017) Dietary hemoglobin rescues young piglets from severe iron deficiency anemia: duodenal expression profile of genes involved in heme iron absorption. PLoS One 12(7):e0181117CrossRefPubMedPubMedCentralGoogle Scholar
  32. 32.
    Rivera S, Nemeth E, Gabayan V, Lopez MA, Farshidi D, Ganz T (2005) Synthetic hepcidin causes rapid dose-dependent hypoferremia and is concentrated in ferroportin-containing organs. Blood 106(6):2196–2199CrossRefPubMedPubMedCentralGoogle Scholar
  33. 33.
    Jordan JB, Poppe L, Haniu M, Arvedson T, Syed R, Li V, Kohno H, Kim H, Schnier PD, Harvey TS, Miranda LP, Cheetham J, Sasu BJ (2009) Hepcidin revisited, disulfide connectivity, dynamics, and structure. J Biol Chem 284(36):24155–24167CrossRefPubMedPubMedCentralGoogle Scholar
  34. 34.
    Andrews NC (2008) Forging a field: the golden age of iron biology. Blood 112(2):219–230CrossRefPubMedPubMedCentralGoogle Scholar
  35. 35.
    Babitt JL, Lin HY (2010) Molecular mechanisms of hepcidin regulation: implications for the anemia of CKD. Am J Kidney Dis 55(4):726–741CrossRefPubMedPubMedCentralGoogle Scholar
  36. 36.
    Camaschella C, Silvestri L (2011) Molecular mechanisms regulating hepcidin revealed by hepcidin disorders. Sci World J 11:1357–1366CrossRefGoogle Scholar
  37. 37.
    Ganz T, Nemeth E (2012) Hepcidin and iron homeostasis. Biochim Biophys Acta 1823(9):1434–1443CrossRefPubMedPubMedCentralGoogle Scholar
  38. 39.
    Tiker F, Celik B, Tarcan A, Kilicdag H, Özbek N, Gurakan B (2006) Serum pro-hepcidin levels and relationships with iron parameters in healthy preterm and term newborns. Pediatr Hematol Oncol 23(4):293–297CrossRefPubMedGoogle Scholar
  39. 40.
    Van Santen S, de Mast Q, Luty AJ, Wiegerinck ET, Van der Ven AJ, Swinkels DW (2011) Iron homeostasis in mother and child during placental malaria infection. Am J Trop Med Hyg 84(1):148–151CrossRefPubMedPubMedCentralGoogle Scholar
  40. 41.
    Fuqua BK, Vulpe CD, Anderson GJ (2012) Intestinal iron absorption. J Trace Elem Med Biol 26(2):115–119CrossRefPubMedGoogle Scholar
  41. 42.
    Li Y, Hansen SL, Borst LB, Spears JW, Moeser AJ (2016) Dietary iron deficiency and oversupplementation increase intestinal permeability, ion transport, and inflammation in pigs. J Nutr 146(8):1499–1505CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Animal Nutrition & Feed Science, College of Animal ScienceZhejiang UniversityHangzhouChina
  2. 2.College of Animal Science and TechnologyAnhui Agricultural UniversityHefeiChina

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