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Alleviation Mechanisms of Selenium on Cadmium-Spiked in Chicken Ovarian Tissue: Perspectives from Autophagy and Energy Metabolism

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Abstract

Cadmium (Cd) is a kind of toxic heavy metal and it can cause damage to organs and tissues. Selenium (Se) can antagonize some metal element toxicity including Cd. The present study was designed to investigate Cd-induced damage to chicken ovary by autophagy and the protective mechanism of Se on Cd-induced damage. Administration of Cd for 12 weeks led to energy metabolism disorder of the chicken ovarian tissues, which resulted in autophagy. In addition, the mRNA expression of glucose-related genes including hexokinase II (HK2), pyruvate kinase (PK), pyruvate dehydrogenase complex (PDHX), and succinate dehydrogenase (SDH) and the activities of ATPase, including Na+-K+-ATPase, Ca2+-ATPase, Mg2+-ATPase, were all downregulated remarkably compared with the control. However, combined with oral administration of Se at 2 mg/kg, the mRNA expression of glucose-related genes and the activities of ATPase increased. The mRNA expression of the autophagy-related genes by Cd treatment, including microtubule-associated protein light chain 3 (LC3), dynein, autophagy-related gene 5 (Atg5), and Beclin 1, was remarkably enhanced, whereas mammalian target of rapamycin (mTOR) was downregulated. However, besides mTOR, their levels displayed a downregulated trend beyond simultaneous Se treatment. The protein expression of autophagy genes was similar to those of mRNA. In conclusion, Cd toxicity affect energy metabolism and induce autophagy, which causes damage to chicken ovary, whereas Se could protect effectively this injury induced by Cd.

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Abbreviations

Cd:

Cadmium

Se:

Selenium

HK2:

Hexokinase II

PK:

Pyruvate kinase

PDHX:

Pyruvate dehydrogenase complex

SDH:

Succinate dehydrogenase

LC3:

Microtubule-associated protein light chain 3

mTOR:

Mammalian target of rapamycin

Atg5:

Autophagy-related gene 5

q-PCR:

Quantitative real-time polymerase chain reaction

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Acknowledgments

The authors thank the members of the Veterinary Internal Medicine Laboratory, College of Veterinary Medicine, Northeast Agriculture University, for their help with sample collection.

Funding

This study was supported by the National Natural Science Foundation of China (Grant No.31472161) and Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine.

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Shuang Wang, Zhe Xu, Hang Yin, Yahong Min & Shu Li

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  1. Shuang Wang
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  2. Zhe Xu
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Correspondence to Shu Li.

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Wang, S., Xu, Z., Yin, H. et al. Alleviation Mechanisms of Selenium on Cadmium-Spiked in Chicken Ovarian Tissue: Perspectives from Autophagy and Energy Metabolism. Biol Trace Elem Res 186, 521–528 (2018). https://doi.org/10.1007/s12011-018-1341-y

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